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The map label for this gene is proS [H]

Identifier: 21674311

GI number: 21674311

Start: 1394666

End: 1396111

Strand: Direct

Name: proS [H]

Synonym: CT1491

Alternate gene names: 21674311

Gene position: 1394666-1396111 (Clockwise)

Preceding gene: 21674309

Following gene: 21674313

Centisome position: 64.72

GC content: 59.27

Gene sequence:

>1446_bases
GTGGCTGATAAAATCACTTCGAGGCAAGAGGACTATTCTCAATGGTATATCGACCTTGTCCGGTCAGCAAAACTGGCCGA
CTATTCGGACGTCCGTGGCTGCATGGTCATCCGCCCGAACGGCTACGCCATCTGGGAAAAGATGCAGGCGGCGCTGGACG
GCATGTTCAAGCAGACCGGCCACGTGAACGCCTACTTCCCGATGTTCATCCCCGAAAGCTTCATCGCCAAGGAGGCCGAG
CACATCGAGGGATTCGCACCGGAGTGCGCCGTGGTAACCCATGGCGGCGGCGAGGAGCTGGCTGAAAAACTCTACGTCCG
CCCCACCTCGGAGACCATCATCTGGTCATCATATAAAAAATGGATACAGTCGTACCGCGACCTGCCGATTCTCATCAACC
AGTGGGCCAACGTCGTTCGCTGGGAGATGCGCACCCGCCTGTTCCTGAGAACCACCGAGTTCCTCTGGCAGGAGGGGCAC
ACCGCCCACGCCACGCCGGAGGAGGCACAGGAGGAGGTGATCCGCATGATCAACATCTATCGCACCTTCGCCGAGGAGTA
CATGGCAATGCCGGTGATCGTCGGCAAAAAGAGCGAGAGCGAGAAGTTCGCAGGCGCAGACGCCACCTACTGCATCGAAG
CAATGATGCAGGACGGTAAGGCGCTGCAGGCGGGCACCTCGCACAACCTCGGCCAAAACTTTGCCAAGGCGTTCGACTGC
CAGTTCCAGACCAAGGACGGCGTCCTCGACTACGTCTGGGCGACGAGCTGGGGCGTTTCGACGCGGCTGATCGGCGCGCT
CATCATGGCGCACTCCGACGACAAGGGCCTCGTACTGCCACCGAAACTCGCCTCGCGGCAGGTGGTGATCATCCCGATCC
TCAAGGGCAACAAGGACGAAGTTCGCGCCCGTGCGCGCTTCATCGCCAAAACTCTGAACAGGCATGGCATCCCGACCTTC
GTCGATGACAGCGAAAACAACTCGCCCGGCTGGAAGTTCGCCGAGTACGAATTGCAGGGCATCCCGGTCAGGATCGAGCT
TGGTCCGCGCGACCTCGAGCAGGGCAAATGCATCGTCGCTCGCCGCGACACGTTCGAGAAGACCGAGTTGCTGCTTGACG
ACGAACTGACAATCAACATCGAGGAGATTCTCAACAACATCCAGCAGAACCTCTACGACCGTGCCCTGCAATTCAGGCTG
GACAACACCGTCGAAGCAACGACTTGGGAAGAGTTCAAGGCAAGCGTGGAGAAAGGCTTCGTCATCGCCCACTGGGACGG
AACGCACGAAACCGAAGCGCTCATCAAGGAGGAGACCAAAGCCACGATCCGGGTAATACCAACCGACGAAGAATACCGCC
AGCAATACAACATGGACGAGCCGGGCACCTGCATCCGCAGCGGCAAACCGGCAGCGCAGAAGGTGGTATTCGCCAAGGCG
TACTGA

Upstream 100 bases:

>100_bases
ACGATGTTATCTGCTTTCATTTCATTACTTTTACAAATCGCATTGGTGCGCGGGCTCGCCACCGCCGTATTTTCCTACAA
TTCAAATCACAAGCATTACC

Downstream 100 bases:

>100_bases
TCTGAATTGCAAAAAAAGGCAGGCGCAACGCCTGCTCCTGTGCTCTTCATTCCTGTCCAGCTCGTTTGCTGTCTTCCACA
AAAAACGCCTCCCCCGCGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MADKITSRQEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKQTGHVNAYFPMFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHATPEEAQEEVIRMINIYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
QFQTKDGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGNKDEVRARARFIAKTLNRHGIPTF
VDDSENNSPGWKFAEYELQGIPVRIELGPRDLEQGKCIVARRDTFEKTELLLDDELTINIEEILNNIQQNLYDRALQFRL
DNTVEATTWEEFKASVEKGFVIAHWDGTHETEALIKEETKATIRVIPTDEEYRQQYNMDEPGTCIRSGKPAAQKVVFAKA
Y

Sequences:

>Translated_481_residues
MADKITSRQEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKQTGHVNAYFPMFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHATPEEAQEEVIRMINIYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
QFQTKDGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGNKDEVRARARFIAKTLNRHGIPTF
VDDSENNSPGWKFAEYELQGIPVRIELGPRDLEQGKCIVARRDTFEKTELLLDDELTINIEEILNNIQQNLYDRALQFRL
DNTVEATTWEEFKASVEKGFVIAHWDGTHETEALIKEETKATIRVIPTDEEYRQQYNMDEPGTCIRSGKPAAQKVVFAKA
Y
>Mature_480_residues
ADKITSRQEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKQTGHVNAYFPMFIPESFIAKEAEH
IEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHT
AHATPEEAQEEVIRMINIYRTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDCQ
FQTKDGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGNKDEVRARARFIAKTLNRHGIPTFV
DDSENNSPGWKFAEYELQGIPVRIELGPRDLEQGKCIVARRDTFEKTELLLDDELTINIEEILNNIQQNLYDRALQFRLD
NTVEATTWEEFKASVEKGFVIAHWDGTHETEALIKEETKATIRVIPTDEEYRQQYNMDEPGTCIRSGKPAAQKVVFAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=494, Percent_Identity=39.6761133603239, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1786392, Length=135, Percent_Identity=31.1111111111111, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71991342, Length=446, Percent_Identity=42.8251121076233, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=46.4985994397759, Blast_Score=355, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=501, Percent_Identity=41.5169660678643, Blast_Score=396, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24649468, Length=443, Percent_Identity=40.4063205417607, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24649466, Length=443, Percent_Identity=40.4063205417607, Blast_Score=354, Evalue=7e-98,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54951; Mature: 54820

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKITSRQEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKQTG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCCEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEHHHHHH
WIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHATPEEAQEEVIRMINIY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
HHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
QFQTKDGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGNKDE
CEECCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCCEECCCCCCCCEEEEEEECCCHHH
VRARARFIAKTLNRHGIPTFVDDSENNSPGWKFAEYELQGIPVRIELGPRDLEQGKCIVA
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEEEE
RRDTFEKTELLLDDELTINIEEILNNIQQNLYDRALQFRLDNTVEATTWEEFKASVEKGF
ECCCCHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCE
VIAHWDGTHETEALIKEETKATIRVIPTDEEYRQQYNMDEPGTCIRSGKPAAQKVVFAKA
EEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCHHCCCCCHHHEEEEEEC
Y
C
>Mature Secondary Structure 
ADKITSRQEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKQTG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCCEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEHHHHHH
WIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHATPEEAQEEVIRMINIY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RTFAEEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDC
HHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
QFQTKDGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILKGNKDE
CEECCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCCEECCCCCCCCEEEEEEECCCHHH
VRARARFIAKTLNRHGIPTFVDDSENNSPGWKFAEYELQGIPVRIELGPRDLEQGKCIVA
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEEEE
RRDTFEKTELLLDDELTINIEEILNNIQQNLYDRALQFRLDNTVEATTWEEFKASVEKGF
ECCCCHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCE
VIAHWDGTHETEALIKEETKATIRVIPTDEEYRQQYNMDEPGTCIRSGKPAAQKVVFAKA
EEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCCCHHCCCCCHHHEEEEEEC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA