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The map label for this gene is yugF [H]

Identifier: 21674135

GI number: 21674135

Start: 1232761

End: 1233612

Strand: Direct

Name: yugF [H]

Synonym: CT1312

Alternate gene names: 21674135

Gene position: 1232761-1233612 (Clockwise)

Preceding gene: 21674131

Following gene: 21674136

Centisome position: 57.21

GC content: 56.69

Gene sequence:

>852_bases
ATGCCAATCCGGAGCAGATATATTACTCTCGGCGGTCACCGCCACCGTTATATCGACACCGGAGGCAACGCACCCGTGAT
GCTGCTGCTTCACGGCATCTCCTCATCGGCTGATTATTATGGCCCATCGATGTCGTTGCTTGCCCGCTCATTCAGAGTGC
TTGGCCTCGACCTGCTCGGCTTCGGTGAATCGGACAAGCCGCGGACGATTCCCTACACCTTGCAGCTCTATGCCGACCTC
ATTCATGAATTCCTCTGGGAAACTGATGCGTTTGCTCATGGCGAGGTTTATGGCACCGGGCATTCGATGGGCGGAAAGTA
TCTGCTCGCAACCGCACTGCTCTACCCCGGCACCTTCAAAAAAATGGTGCTCAGCAACACGGACGGCTTCATTGTCCTGC
CCTCGTTCGCCAGGGCGATCAGCCTGCCGGGCGTGCGTCATGTGCTCAAGCCGCTCGTTACCGGCGAGCGCATTGCGGCC
AAAATGCTCGACATGGCGATTCACAACCGGCAGGCCATCGACGACGAGACGTACCGTAAAGTGCTCCAGATCGCGCGAGA
CCACGACGCGTTCGAGACCGTCATGAGCCTAAACCGCAACATGCTGAAGCTCGACCTGAAGCGCACCGGCCTGCGAGCGA
GGCTCCGGGAGCTGAAACAACCGGTACTGATCATCTGGGGAGAACACGACCGCTACATCTCGCCGAAAATTGCCCACATC
GTCAAGCGGGAACTGCCTCACGCAAAGCTGCTCATTTTTAAAGATTGCGGCCACTCTCCAATGCTCGAATATCCGGAACA
GTTCAGCACAGCAATCACGGAATTCATTCACCAGGAACCACCATTGCCTTAA

Upstream 100 bases:

>100_bases
GTCCTCCTGTGCGCGTATGCTCTTTTTTTGGTGATCATTGATTATTTTTGAGTGCTGCCAGACAGGCCTCGCGACTATCA
CCTCAACAAACCGCCCGTCC

Downstream 100 bases:

>100_bases
CCTGTTATGCTCATTTCATTTGAAGGAATCGACGGCGCAGGAAAATCAACCCAGGTCATGAAGCTCAAACGCTATCTTCA
AGAGCGAGGGCGCGAAGTTC

Product: lipase, putative

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPIRSRYITLGGHRHRYIDTGGNAPVMLLLHGISSSADYYGPSMSLLARSFRVLGLDLLGFGESDKPRTIPYTLQLYADL
IHEFLWETDAFAHGEVYGTGHSMGGKYLLATALLYPGTFKKMVLSNTDGFIVLPSFARAISLPGVRHVLKPLVTGERIAA
KMLDMAIHNRQAIDDETYRKVLQIARDHDAFETVMSLNRNMLKLDLKRTGLRARLRELKQPVLIIWGEHDRYISPKIAHI
VKRELPHAKLLIFKDCGHSPMLEYPEQFSTAITEFIHQEPPLP

Sequences:

>Translated_283_residues
MPIRSRYITLGGHRHRYIDTGGNAPVMLLLHGISSSADYYGPSMSLLARSFRVLGLDLLGFGESDKPRTIPYTLQLYADL
IHEFLWETDAFAHGEVYGTGHSMGGKYLLATALLYPGTFKKMVLSNTDGFIVLPSFARAISLPGVRHVLKPLVTGERIAA
KMLDMAIHNRQAIDDETYRKVLQIARDHDAFETVMSLNRNMLKLDLKRTGLRARLRELKQPVLIIWGEHDRYISPKIAHI
VKRELPHAKLLIFKDCGHSPMLEYPEQFSTAITEFIHQEPPLP
>Mature_282_residues
PIRSRYITLGGHRHRYIDTGGNAPVMLLLHGISSSADYYGPSMSLLARSFRVLGLDLLGFGESDKPRTIPYTLQLYADLI
HEFLWETDAFAHGEVYGTGHSMGGKYLLATALLYPGTFKKMVLSNTDGFIVLPSFARAISLPGVRHVLKPLVTGERIAAK
MLDMAIHNRQAIDDETYRKVLQIARDHDAFETVMSLNRNMLKLDLKRTGLRARLRELKQPVLIIWGEHDRYISPKIAHIV
KRELPHAKLLIFKDCGHSPMLEYPEQFSTAITEFIHQEPPLP

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=265, Percent_Identity=22.6415094339623, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI145611434, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87081721, Length=275, Percent_Identity=23.6363636363636, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32028; Mature: 31897

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIRSRYITLGGHRHRYIDTGGNAPVMLLLHGISSSADYYGPSMSLLARSFRVLGLDLLG
CCCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEC
FGESDKPRTIPYTLQLYADLIHEFLWETDAFAHGEVYGTGHSMGGKYLLATALLYPGTFK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHH
KMVLSNTDGFIVLPSFARAISLPGVRHVLKPLVTGERIAAKMLDMAIHNRQAIDDETYRK
HHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
VLQIARDHDAFETVMSLNRNMLKLDLKRTGLRARLRELKQPVLIIWGEHDRYISPKIAHI
HHHHHHCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
VKRELPHAKLLIFKDCGHSPMLEYPEQFSTAITEFIHQEPPLP
HHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PIRSRYITLGGHRHRYIDTGGNAPVMLLLHGISSSADYYGPSMSLLARSFRVLGLDLLG
CCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEC
FGESDKPRTIPYTLQLYADLIHEFLWETDAFAHGEVYGTGHSMGGKYLLATALLYPGTFK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHH
KMVLSNTDGFIVLPSFARAISLPGVRHVLKPLVTGERIAAKMLDMAIHNRQAIDDETYRK
HHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
VLQIARDHDAFETVMSLNRNMLKLDLKRTGLRARLRELKQPVLIIWGEHDRYISPKIAHI
HHHHHHCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
VKRELPHAKLLIFKDCGHSPMLEYPEQFSTAITEFIHQEPPLP
HHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]