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The map label for this gene is rfbD [H]

Identifier: 21673146

GI number: 21673146

Start: 320886

End: 321764

Strand: Direct

Name: rfbD [H]

Synonym: CT0307

Alternate gene names: 21673146

Gene position: 320886-321764 (Clockwise)

Preceding gene: 21673145

Following gene: 21673147

Centisome position: 14.89

GC content: 55.97

Gene sequence:

>879_bases
ATGAATATTCTTGTCACCGGGAGCCGTGGGCAGCTTGGTTCCGAACTGCAAAAACTGCAAGAAGTGCATGGCTGGCAGGA
GTGGTTTTTCATGGATCTGCCGGAACTCGACATTACCGATGCGCTGGCGGTGGAGCGTGTTTGCCGGGATCGACGGATTG
GCGCGATTGTCAACTGTGCGGCCTATACGGCGGTGGACAGGGCTGAGAGCGATGCGGAAGCAGCGTTCAGAGTGAACCGT
GACGGCGCGGCGGTGCTGGCCGCGGTTGCAATGGAGGTTGGCGCACTGTTGCTGCATGTTTCGACCGATTATGTTTTCGA
TGGCAGCTCGAACCGACCTTATTGCGAGGATGACCCGGTTGCGCCTTGCGGTGTTTATGGCCTGTCCAAATGGGAGGGTG
AAGAGGCGATCCGGGCGAGCGGCTGCTCGTATATCATTCTACGCACCGCCTGGCTCTACTCGGTTTACGGGCAGAACTTC
GTGAAGACTATGCTTCGTCTTGGTAGCGAGCGCCAGTCGCTCGGCGTTGTGTTTGATCAGGTGGGCAGTCCGACATGGGC
GGCGGATCTGGCCGGGACGATCGTTTCGATACTCGACCAATGCGATCCGGTTCGGAGCTACAGCGAGACTTTTCACTACT
CGAATGAAGGAGTCTGTTCCTGGTACGACTTTGCCAAATCGATTATGGATGCCGAAGGGCTTTCGTGCAAGGTGTTGCCA
ATCGAGAGCAGTAACTATCCGACGCCAGCCAGAAGGCCGCATTTCAGTGTGCTGAACAAACGAAAAATCAAGAGTACATT
GGGGCTTGAAATCCCGTACTGGCACGACAGTCTGTTGCGGATGCTGACAGAGTTGCGGAAGACGGCTGGCAAATCGTAA

Upstream 100 bases:

>100_bases
TTCGGATAAGGATCTTCATCATCCGATGCTGCACGAGATTGAAGGTATTGTGCTGGACGCGTAGCATATTTTTCGAACCG
CTTATACCGACCAGAGAACA

Downstream 100 bases:

>100_bases
GTTTGGCTGCTATGGAACGGAATGGGTCAGGAAGACAAAAGGCGATGCTTCTGAATCATTATCAGTTATCAACTATCAAA
TTGCTTCGATGCATATTCTC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNILVTGSRGQLGSELQKLQEVHGWQEWFFMDLPELDITDALAVERVCRDRRIGAIVNCAAYTAVDRAESDAEAAFRVNR
DGAAVLAAVAMEVGALLLHVSTDYVFDGSSNRPYCEDDPVAPCGVYGLSKWEGEEAIRASGCSYIILRTAWLYSVYGQNF
VKTMLRLGSERQSLGVVFDQVGSPTWAADLAGTIVSILDQCDPVRSYSETFHYSNEGVCSWYDFAKSIMDAEGLSCKVLP
IESSNYPTPARRPHFSVLNKRKIKSTLGLEIPYWHDSLLRMLTELRKTAGKS

Sequences:

>Translated_292_residues
MNILVTGSRGQLGSELQKLQEVHGWQEWFFMDLPELDITDALAVERVCRDRRIGAIVNCAAYTAVDRAESDAEAAFRVNR
DGAAVLAAVAMEVGALLLHVSTDYVFDGSSNRPYCEDDPVAPCGVYGLSKWEGEEAIRASGCSYIILRTAWLYSVYGQNF
VKTMLRLGSERQSLGVVFDQVGSPTWAADLAGTIVSILDQCDPVRSYSETFHYSNEGVCSWYDFAKSIMDAEGLSCKVLP
IESSNYPTPARRPHFSVLNKRKIKSTLGLEIPYWHDSLLRMLTELRKTAGKS
>Mature_292_residues
MNILVTGSRGQLGSELQKLQEVHGWQEWFFMDLPELDITDALAVERVCRDRRIGAIVNCAAYTAVDRAESDAEAAFRVNR
DGAAVLAAVAMEVGALLLHVSTDYVFDGSSNRPYCEDDPVAPCGVYGLSKWEGEEAIRASGCSYIILRTAWLYSVYGQNF
VKTMLRLGSERQSLGVVFDQVGSPTWAADLAGTIVSILDQCDPVRSYSETFHYSNEGVCSWYDFAKSIMDAEGLSCKVLP
IESSNYPTPARRPHFSVLNKRKIKSTLGLEIPYWHDSLLRMLTELRKTAGKS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=280, Percent_Identity=32.1428571428571, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI11034825, Length=280, Percent_Identity=32.1428571428571, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788352, Length=296, Percent_Identity=38.5135135135135, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32397; Mature: 32397

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGSRGQLGSELQKLQEVHGWQEWFFMDLPELDITDALAVERVCRDRRIGAIVNCA
CCEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
AYTAVDRAESDAEAAFRVNRDGAAVLAAVAMEVGALLLHVSTDYVFDGSSNRPYCEDDPV
HHHHHHHCCCCHHHHEEECCCHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCC
APCGVYGLSKWEGEEAIRASGCSYIILRTAWLYSVYGQNFVKTMLRLGSERQSLGVVFDQ
CCCCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGSPTWAADLAGTIVSILDQCDPVRSYSETFHYSNEGVCSWYDFAKSIMDAEGLSCKVLP
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
IESSNYPTPARRPHFSVLNKRKIKSTLGLEIPYWHDSLLRMLTELRKTAGKS
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILVTGSRGQLGSELQKLQEVHGWQEWFFMDLPELDITDALAVERVCRDRRIGAIVNCA
CCEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
AYTAVDRAESDAEAAFRVNRDGAAVLAAVAMEVGALLLHVSTDYVFDGSSNRPYCEDDPV
HHHHHHHCCCCHHHHEEECCCHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCC
APCGVYGLSKWEGEEAIRASGCSYIILRTAWLYSVYGQNFVKTMLRLGSERQSLGVVFDQ
CCCCCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGSPTWAADLAGTIVSILDQCDPVRSYSETFHYSNEGVCSWYDFAKSIMDAEGLSCKVLP
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
IESSNYPTPARRPHFSVLNKRKIKSTLGLEIPYWHDSLLRMLTELRKTAGKS
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]