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The map label for this gene is rfbB [H]

Identifier: 21673147

GI number: 21673147

Start: 321853

End: 322902

Strand: Direct

Name: rfbB [H]

Synonym: CT0308

Alternate gene names: 21673147

Gene position: 321853-322902 (Clockwise)

Preceding gene: 21673146

Following gene: 21673148

Centisome position: 14.94

GC content: 55.9

Gene sequence:

>1050_bases
ATGCATATTCTCATTACCGGTGGTGCGGGGTTTATTGGTTCGCATGTGGTCAGGCACTTTTTGAATCGTTATGCGGACTA
CACGATCACCAATCTCGACAAGCTTACCTATGCGGGCAATCTGGCCAATCTGAAAGATGTCGAATCGAATCCGAACTACC
GGTTCGTGAAGGGCGATATCGCTGATGGCGCATTTTTGCTCGATCTGTTCAAAGAGCAGCGCTTCGATGCGGTCATCCAT
CTGGCCGCCGAGTCGCACGTGGATCGCTCCATCGAAAGCCCGGTCGAGTTCGTGATTACCAACGTGTTTGGCACGGTGAA
CCTGCTCAACGCCGCGCGTGCCACATGGGAAGGCAGGTTCGAGGGGAAGCGGTTTTACCACATTTCGACCGACGAAGTCT
ATGGTTCGCTCGGCAGCGAGGGGATGTTTAGCGAATCGACCCCCTACGATCCGCATAGTCCCTACTCGGCCTCGAAAGCG
TCGTCGGATCACTTCGTCCGGGCATTCCACGCTACTTACGGCCTGCCGGTGGTTATCAGCAACTGTTCGAACAACTACGG
TTCGCACCAGTTTCCCGAAAAGCTGATTCCGCTTTTCATCAACAACATCCGTCTGGAGAAGCCGCTTCCGGTGTATGGTC
AAGGCCTGAACGTTCGCGACTGGCTTTGGGTGGTTGATCACGCACGAGCCATTGACGAGATTTTCCACCGCGGAGCGGTG
GGCGAGACCTACAACATCGGCGGGCACAACGAGTGGACGAACATCGACCTGATCCGCCTGCTCTGCCGCATCATGGACCG
CAAGCTTGGCCGCGAGGCGGGAAGTTCCGAAAAGCTCATCACCTGGGTGACCGACCGCGCGGGCCACGACTTGCGCTATG
CCATTGATGCCTCAAAGCTGCAACGTGAGCTCGGATGGGCGCCGTCCGTGACCTTCGAGGAGGGGCTGGAAAAAACCGTT
GACTGGTATCTTGAAAATCAAGCATGGCTCGATGAGGTTACGTCGGGAGCCTATCAGCACTATTATGAAAAGATGTACGC
TGGCCGCTGA

Upstream 100 bases:

>100_bases
GGCAAATCGTAAGTTTGGCTGCTATGGAACGGAATGGGTCAGGAAGACAAAAGGCGATGCTTCTGAATCATTATCAGTTA
TCAACTATCAAATTGCTTCG

Downstream 100 bases:

>100_bases
GTGTGGTTGTGACGTTCAATTCTCGGTAACCATTCCATCGATTTTGCAATGATGATCATGCCTGTCATTCTTTCCGGCGG
TTCCGGAACGAGGCTCTGGC

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MHILITGGAGFIGSHVVRHFLNRYADYTITNLDKLTYAGNLANLKDVESNPNYRFVKGDIADGAFLLDLFKEQRFDAVIH
LAAESHVDRSIESPVEFVITNVFGTVNLLNAARATWEGRFEGKRFYHISTDEVYGSLGSEGMFSESTPYDPHSPYSASKA
SSDHFVRAFHATYGLPVVISNCSNNYGSHQFPEKLIPLFINNIRLEKPLPVYGQGLNVRDWLWVVDHARAIDEIFHRGAV
GETYNIGGHNEWTNIDLIRLLCRIMDRKLGREAGSSEKLITWVTDRAGHDLRYAIDASKLQRELGWAPSVTFEEGLEKTV
DWYLENQAWLDEVTSGAYQHYYEKMYAGR

Sequences:

>Translated_349_residues
MHILITGGAGFIGSHVVRHFLNRYADYTITNLDKLTYAGNLANLKDVESNPNYRFVKGDIADGAFLLDLFKEQRFDAVIH
LAAESHVDRSIESPVEFVITNVFGTVNLLNAARATWEGRFEGKRFYHISTDEVYGSLGSEGMFSESTPYDPHSPYSASKA
SSDHFVRAFHATYGLPVVISNCSNNYGSHQFPEKLIPLFINNIRLEKPLPVYGQGLNVRDWLWVVDHARAIDEIFHRGAV
GETYNIGGHNEWTNIDLIRLLCRIMDRKLGREAGSSEKLITWVTDRAGHDLRYAIDASKLQRELGWAPSVTFEEGLEKTV
DWYLENQAWLDEVTSGAYQHYYEKMYAGR
>Mature_349_residues
MHILITGGAGFIGSHVVRHFLNRYADYTITNLDKLTYAGNLANLKDVESNPNYRFVKGDIADGAFLLDLFKEQRFDAVIH
LAAESHVDRSIESPVEFVITNVFGTVNLLNAARATWEGRFEGKRFYHISTDEVYGSLGSEGMFSESTPYDPHSPYSASKA
SSDHFVRAFHATYGLPVVISNCSNNYGSHQFPEKLIPLFINNIRLEKPLPVYGQGLNVRDWLWVVDHARAIDEIFHRGAV
GETYNIGGHNEWTNIDLIRLLCRIMDRKLGREAGSSEKLITWVTDRAGHDLRYAIDASKLQRELGWAPSVTFEEGLEKTV
DWYLENQAWLDEVTSGAYQHYYEKMYAGR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=40.8536585365854, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=25.6716417910448, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI56237023, Length=353, Percent_Identity=25.4957507082153, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI56118217, Length=353, Percent_Identity=25.4957507082153, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI189083684, Length=353, Percent_Identity=25.4957507082153, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=24.3553008595989, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=54.1436464088398, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=56.5217391304348, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=27.0114942528736, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=26.685393258427, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=36.4457831325301, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=33.9506172839506, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71982035, Length=359, Percent_Identity=28.6908077994429, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=361, Percent_Identity=28.5318559556787, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=24.4648318042813, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI133901788, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=164, Percent_Identity=33.5365853658537, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=23.546511627907, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=27.6536312849162, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=25.561797752809, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=345, Percent_Identity=23.768115942029, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24158427, Length=356, Percent_Identity=23.876404494382, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39605; Mature: 39605

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILITGGAGFIGSHVVRHFLNRYADYTITNLDKLTYAGNLANLKDVESNPNYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHHHCCCEECCHHHHHCCCCCCCCHHCCCCCCEEEEECCC
ADGAFLLDLFKEQRFDAVIHLAAESHVDRSIESPVEFVITNVFGTVNLLNAARATWEGRF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGKRFYHISTDEVYGSLGSEGMFSESTPYDPHSPYSASKASSDHFVRAFHATYGLPVVIS
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEE
NCSNNYGSHQFPEKLIPLFINNIRLEKPLPVYGQGLNVRDWLWVVDHARAIDEIFHRGAV
CCCCCCCCCCCHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GETYNIGGHNEWTNIDLIRLLCRIMDRKLGREAGSSEKLITWVTDRAGHDLRYAIDASKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHH
QRELGWAPSVTFEEGLEKTVDWYLENQAWLDEVTSGAYQHYYEKMYAGR
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHILITGGAGFIGSHVVRHFLNRYADYTITNLDKLTYAGNLANLKDVESNPNYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHHHCCCEECCHHHHHCCCCCCCCHHCCCCCCEEEEECCC
ADGAFLLDLFKEQRFDAVIHLAAESHVDRSIESPVEFVITNVFGTVNLLNAARATWEGRF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGKRFYHISTDEVYGSLGSEGMFSESTPYDPHSPYSASKASSDHFVRAFHATYGLPVVIS
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEE
NCSNNYGSHQFPEKLIPLFINNIRLEKPLPVYGQGLNVRDWLWVVDHARAIDEIFHRGAV
CCCCCCCCCCCHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GETYNIGGHNEWTNIDLIRLLCRIMDRKLGREAGSSEKLITWVTDRAGHDLRYAIDASKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHH
QRELGWAPSVTFEEGLEKTVDWYLENQAWLDEVTSGAYQHYYEKMYAGR
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]