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The map label for this gene is rho [H]

Identifier: 21673103

GI number: 21673103

Start: 274266

End: 275555

Strand: Direct

Name: rho [H]

Synonym: CT0264

Alternate gene names: 21673103

Gene position: 274266-275555 (Clockwise)

Preceding gene: 21673102

Following gene: 21673104

Centisome position: 12.73

GC content: 54.34

Gene sequence:

>1290_bases
ATGTCGAACAATTCGGTATCCAAAGGCCTGGACATCAATGCACTCCAGAAGAAAAAGGTTCATGAACTCAATGCCATCGC
CAAAGAGCTTGGCGTGATTACTGCCGGTTTTCGGAAGGAGGAGTTAATCTACAAGATTATCGAGGCGCAGTCCCTGAAAA
ATCCTGATTCCGAGAGTGGACAGGTAATGGTCAATACCGGAGTGTTGCAGGTCATACCCGAAGGCTACGGTTTCCTGCGA
TCCTCAAACTACAACTACCTCTCTTCCCCCGACGACATCTACGTTTCTCCTTCACAGATCAAGCGTTTCAACATGCGAAC
CGGCGACACCGTCTCCGGTCAGGTCAGGGCGCCGAAAGAGGGAGAGCGCTTCTTTGCGCTGCTGAAGATCAACACCATCA
ACGGAAAGGATCCCGAAGTGACCCGCGAGCGTCCTTTCTTTGAGAACCTGACGCCGCTCTTTCCCCATTCGCGTCTCAAG
CTCGAAACCCGTCAGAACGAGTACTCCGGGCGCATCATGGATATTTTCACGCCCATCGGCAAAGGCCAGCGCGGCCTGAT
CGTCGCCCAGCCGAAGACCGGTAAGACCATTCTGTTGCAGATGATCGCCAACGCCATCATCAAAAACCATCCGGAGGTTT
ATCTCATCGTACTGCTTATCGACGAGCGCCCTGAGGAGGTGACCGACATGGCTCGCAGCGTGGAAGCGGAGGTGGTCAGC
TCGACCTTCGATGAGGATCCGGAGCGGCACGTGCAGGTGGCCGACATGGTGCTTGAAAAGGCCAAGAGGCTGGTTGAGGT
TGGTCACGACGTGGTGATTCTGCTCGACTCTATCACCAGACTCGCCCGCGCGCACAACACCATCATTCCGCATTCCGGCA
AGATTCTCTCCGGTGGTATCGACGCCAACGCGCTCACCAAGCCGAAGCGCTTTTTCGGCGCGGCGCGAAATATCGAGGAG
GGGGGCAGTCTAACCATTATCGCCACGGCGCTGGTCGATACCGGTTCGCGCATGGATGATGTTATCTTCGAAGAGTTCAA
AGGTACTGGTAACATGGAACTTGTGCTCGACCGGCGTCTTTCGGAGCGCAGAATCTTTCCGGCCATCGATATTCTCCGCT
CGGGAACCCGAAAAGAGGAGCTGCTGTTTACCCAGGAAGAGCTGTCGCGTACCTGGCTGCTTCGGAAATACCTCGGCGAC
AAGAACCCCATCGAATGTATGGAGTTCATGCGTGAGAAAATCGTTGAAACCAAGGATAACAAGGAGTTCTTCAAGTACAT
GAATGCCTGA

Upstream 100 bases:

>100_bases
CGGCGGCCATCGACGATGATCCCGTTGTTTCTCCTCAGTGATTACGCATGAGGCAGTTCTTTTACTGCATCCTTAACCAT
TAAGCGTTGACCAATCAATA

Downstream 100 bases:

>100_bases
CCTGATTGATCGGGCTGATTCTGCGAATCCGTCGAATTGGCCCATCGGATTCAACTTGCCGCTGTTTCCGGAGTAAAAGT
GTCCTCCGGTATTTTTAATT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSNNSVSKGLDINALQKKKVHELNAIAKELGVITAGFRKEELIYKIIEAQSLKNPDSESGQVMVNTGVLQVIPEGYGFLR
SSNYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTINGKDPEVTRERPFFENLTPLFPHSRLK
LETRQNEYSGRIMDIFTPIGKGQRGLIVAQPKTGKTILLQMIANAIIKNHPEVYLIVLLIDERPEEVTDMARSVEAEVVS
STFDEDPERHVQVADMVLEKAKRLVEVGHDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEE
GGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELVLDRRLSERRIFPAIDILRSGTRKEELLFTQEELSRTWLLRKYLGD
KNPIECMEFMREKIVETKDNKEFFKYMNA

Sequences:

>Translated_429_residues
MSNNSVSKGLDINALQKKKVHELNAIAKELGVITAGFRKEELIYKIIEAQSLKNPDSESGQVMVNTGVLQVIPEGYGFLR
SSNYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTINGKDPEVTRERPFFENLTPLFPHSRLK
LETRQNEYSGRIMDIFTPIGKGQRGLIVAQPKTGKTILLQMIANAIIKNHPEVYLIVLLIDERPEEVTDMARSVEAEVVS
STFDEDPERHVQVADMVLEKAKRLVEVGHDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEE
GGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELVLDRRLSERRIFPAIDILRSGTRKEELLFTQEELSRTWLLRKYLGD
KNPIECMEFMREKIVETKDNKEFFKYMNA
>Mature_428_residues
SNNSVSKGLDINALQKKKVHELNAIAKELGVITAGFRKEELIYKIIEAQSLKNPDSESGQVMVNTGVLQVIPEGYGFLRS
SNYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTINGKDPEVTRERPFFENLTPLFPHSRLKL
ETRQNEYSGRIMDIFTPIGKGQRGLIVAQPKTGKTILLQMIANAIIKNHPEVYLIVLLIDERPEEVTDMARSVEAEVVSS
TFDEDPERHVQVADMVLEKAKRLVEVGHDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEEG
GSLTIIATALVDTGSRMDDVIFEEFKGTGNMELVLDRRLSERRIFPAIDILRSGTRKEELLFTQEELSRTWLLRKYLGDK
NPIECMEFMREKIVETKDNKEFFKYMNA

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=423, Percent_Identity=59.1016548463357, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1788251, Length=240, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48470; Mature: 48339

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNSVSKGLDINALQKKKVHELNAIAKELGVITAGFRKEELIYKIIEAQSLKNPDSESG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
QVMVNTGVLQVIPEGYGFLRSSNYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKE
EEEEECCEEEECCCCCCEEECCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCC
GERFFALLKINTINGKDPEVTRERPFFENLTPLFPHSRLKLETRQNEYSGRIMDIFTPIG
CCEEEEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCC
KGQRGLIVAQPKTGKTILLQMIANAIIKNHPEVYLIVLLIDERPEEVTDMARSVEAEVVS
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEDPERHVQVADMVLEKAKRLVEVGHDVVILLDSITRLARAHNTIIPHSGKILSGGI
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
DANALTKPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELVLDRRL
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECHH
SERRIFPAIDILRSGTRKEELLFTQEELSRTWLLRKYLGDKNPIECMEFMREKIVETKDN
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
KEFFKYMNA
HHHHHHHCC
>Mature Secondary Structure 
SNNSVSKGLDINALQKKKVHELNAIAKELGVITAGFRKEELIYKIIEAQSLKNPDSESG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
QVMVNTGVLQVIPEGYGFLRSSNYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKE
EEEEECCEEEECCCCCCEEECCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCC
GERFFALLKINTINGKDPEVTRERPFFENLTPLFPHSRLKLETRQNEYSGRIMDIFTPIG
CCEEEEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCC
KGQRGLIVAQPKTGKTILLQMIANAIIKNHPEVYLIVLLIDERPEEVTDMARSVEAEVVS
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEDPERHVQVADMVLEKAKRLVEVGHDVVILLDSITRLARAHNTIIPHSGKILSGGI
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
DANALTKPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELVLDRRL
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECHH
SERRIFPAIDILRSGTRKEELLFTQEELSRTWLLRKYLGDKNPIECMEFMREKIVETKDN
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
KEFFKYMNA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8051015 [H]