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The map label for this gene is folC [H]

Identifier: 21673102

GI number: 21673102

Start: 272797

End: 273975

Strand: Direct

Name: folC [H]

Synonym: CT0263

Alternate gene names: 21673102

Gene position: 272797-273975 (Clockwise)

Preceding gene: 21673101

Following gene: 21673103

Centisome position: 12.66

GC content: 62.43

Gene sequence:

>1179_bases
GTGCATGTCGCCGGAACCAACGGCAAGGGCACGGTAGCTTCGTGCGTTGCTTCGATTTTCAGCACCTCGGGCCGCAAAAC
CGGCCTGTTCACATCGCCGCACCTGGTCGATTTCACGGAGCGGATCCGCATCGACGGCCAGCAGATTGGCCAGGCGCGAG
TCGCTGAATATTGTACCAAGCTGCAACCGGCGGTCGAAACGGGCGCGACCTTTTTCGAAACGACCACGGCGATGGCGTTT
GCCTTTTTCGCCGACGAAGGCGTCGATGCCGCGGTGATCGAAACCGGCATGGGGGGGCGGCTCGATGCGACCAATGTCGT
GCAACCGGAGATTGTTATCATTCCGAGCATCGGCATGGATCACACCGAGTGGCTCGGCGGGAGCCTTCGCGAAATAGCCG
CCGAGAAGGCGGCGATCATCAAGCGGTGCTCGCGCGTTTTCACCGCCGTGCCGGAAGCGGGCGAGGCGTTCGCGCCGATC
CGCGAGGCCGCCGAAGCGGTGGGCGCGGAGCTGCATCAGGTTGAGCGGGAGGCGGAGTGTTTGGTGGAGGAGGTTTGCCC
GGGCGCTCTCGCCCTGCGAATTTCGCTCGATGGTGGCGAGTCGCGGCAGTTTCGGGCTGCGCTCACCGGCTCATTCCATG
CGCCGAACGTTTGCCTTGCCGTCATGGCCGCCCGTTCCGAGGGGATCTCGTGGGAGCATATCGATGATGGGCTGGCAAGG
CTTGGCGCTTCGGGTTACCGGGCACGCCTGGAACGAATCGCGGACAAACCGGTGGTGATGCTTGATGTCTCCCACAATCC
GGAAGGGATGCAGAAAACCGCGCAGTCGATTCTGGAGCTTCGAAACTGCTTCCGCTTCCTGTACGTGATCATTGGCGTTG
CGGCCGACAAGGATGCTGCCGGTATCGTTCACCATATCGCGCCGATTGCCGACGAAATTGTCGCTGTGGATCTGCCGGTG
GAGCGGAGTCTTCAGGCGGAGGTGCTCGAGCGGTTGTGCGTGGAAGCCGGGGCGCAGTATGTCAGCTCGCGCCACTCGGC
TGCCGAGGCGCTGGAATTTCTCGATCAGCGCGTCGAACCCGAAGATATGATTCTGGTGACGGGTTCTTTCTATCTTTCCG
GAGAGGTGGCGGCGATGGAGCGGTTTCGTTCACCGGGTGCATCGGCCGGCGCGATATGA

Upstream 100 bases:

>100_bases
CCTGTTTCCCCTTCATCGTTTTGGCATCAAACCCGGCCTTGAACGTATCGAAGCGCTGCTCGACGTGCTCGGTCATCCGG
AGCGCAAACTCGGCACCATT

Downstream 100 bases:

>100_bases
TTCATCTGTTTTTACATAAAATATTTTATATTCGTGTTGACCTCTGAGATTCTCCAGCCAAGGCAGGAGCCTTCCGCTGG
CGGTTCTCCTGGTAAATACC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MHVAGTNGKGTVASCVASIFSTSGRKTGLFTSPHLVDFTERIRIDGQQIGQARVAEYCTKLQPAVETGATFFETTTAMAF
AFFADEGVDAAVIETGMGGRLDATNVVQPEIVIIPSIGMDHTEWLGGSLREIAAEKAAIIKRCSRVFTAVPEAGEAFAPI
REAAEAVGAELHQVEREAECLVEEVCPGALALRISLDGGESRQFRAALTGSFHAPNVCLAVMAARSEGISWEHIDDGLAR
LGASGYRARLERIADKPVVMLDVSHNPEGMQKTAQSILELRNCFRFLYVIIGVAADKDAAGIVHHIAPIADEIVAVDLPV
ERSLQAEVLERLCVEAGAQYVSSRHSAAEALEFLDQRVEPEDMILVTGSFYLSGEVAAMERFRSPGASAGAI

Sequences:

>Translated_392_residues
MHVAGTNGKGTVASCVASIFSTSGRKTGLFTSPHLVDFTERIRIDGQQIGQARVAEYCTKLQPAVETGATFFETTTAMAF
AFFADEGVDAAVIETGMGGRLDATNVVQPEIVIIPSIGMDHTEWLGGSLREIAAEKAAIIKRCSRVFTAVPEAGEAFAPI
REAAEAVGAELHQVEREAECLVEEVCPGALALRISLDGGESRQFRAALTGSFHAPNVCLAVMAARSEGISWEHIDDGLAR
LGASGYRARLERIADKPVVMLDVSHNPEGMQKTAQSILELRNCFRFLYVIIGVAADKDAAGIVHHIAPIADEIVAVDLPV
ERSLQAEVLERLCVEAGAQYVSSRHSAAEALEFLDQRVEPEDMILVTGSFYLSGEVAAMERFRSPGASAGAI
>Mature_392_residues
MHVAGTNGKGTVASCVASIFSTSGRKTGLFTSPHLVDFTERIRIDGQQIGQARVAEYCTKLQPAVETGATFFETTTAMAF
AFFADEGVDAAVIETGMGGRLDATNVVQPEIVIIPSIGMDHTEWLGGSLREIAAEKAAIIKRCSRVFTAVPEAGEAFAPI
REAAEAVGAELHQVEREAECLVEEVCPGALALRISLDGGESRQFRAALTGSFHAPNVCLAVMAARSEGISWEHIDDGLAR
LGASGYRARLERIADKPVVMLDVSHNPEGMQKTAQSILELRNCFRFLYVIIGVAADKDAAGIVHHIAPIADEIVAVDLPV
ERSLQAEVLERLCVEAGAQYVSSRHSAAEALEFLDQRVEPEDMILVTGSFYLSGEVAAMERFRSPGASAGAI

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=211, Percent_Identity=34.5971563981043, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI66932984, Length=211, Percent_Identity=34.5971563981043, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788654, Length=373, Percent_Identity=31.9034852546917, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=435, Percent_Identity=25.7471264367816, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=435, Percent_Identity=25.7471264367816, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=435, Percent_Identity=25.7471264367816, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=179, Percent_Identity=37.4301675977654, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324815, Length=243, Percent_Identity=29.2181069958848, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641571, Length=182, Percent_Identity=39.5604395604396, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=34.2696629213483, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 41871; Mature: 41871

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVAGTNGKGTVASCVASIFSTSGRKTGLFTSPHLVDFTERIRIDGQQIGQARVAEYCTK
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
LQPAVETGATFFETTTAMAFAFFADEGVDAAVIETGMGGRLDATNVVQPEIVIIPSIGMD
HCHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCCCEEEECCCCCC
HTEWLGGSLREIAAEKAAIIKRCSRVFTAVPEAGEAFAPIREAAEAVGAELHQVEREAEC
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVEEVCPGALALRISLDGGESRQFRAALTGSFHAPNVCLAVMAARSEGISWEHIDDGLAR
HHHHHCCCEEEEEEEECCCCCCEEHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
LGASGYRARLERIADKPVVMLDVSHNPEGMQKTAQSILELRNCFRFLYVIIGVAADKDAA
HCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIVHHIAPIADEIVAVDLPVERSLQAEVLERLCVEAGAQYVSSRHSAAEALEFLDQRVEP
HHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDMILVTGSFYLSGEVAAMERFRSPGASAGAI
CCEEEEEECEEECCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHVAGTNGKGTVASCVASIFSTSGRKTGLFTSPHLVDFTERIRIDGQQIGQARVAEYCTK
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
LQPAVETGATFFETTTAMAFAFFADEGVDAAVIETGMGGRLDATNVVQPEIVIIPSIGMD
HCHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCCCEEEECCCCCC
HTEWLGGSLREIAAEKAAIIKRCSRVFTAVPEAGEAFAPIREAAEAVGAELHQVEREAEC
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVEEVCPGALALRISLDGGESRQFRAALTGSFHAPNVCLAVMAARSEGISWEHIDDGLAR
HHHHHCCCEEEEEEEECCCCCCEEHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
LGASGYRARLERIADKPVVMLDVSHNPEGMQKTAQSILELRNCFRFLYVIIGVAADKDAA
HCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIVHHIAPIADEIVAVDLPVERSLQAEVLERLCVEAGAQYVSSRHSAAEALEFLDQRVEP
HHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDMILVTGSFYLSGEVAAMERFRSPGASAGAI
CCEEEEEECEEECCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]