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The map label for this gene is aegA [H]

Identifier: 161485731

GI number: 161485731

Start: 292662

End: 294623

Strand: Reverse

Name: aegA [H]

Synonym: CT0282

Alternate gene names: 161485731

Gene position: 294623-292662 (Counterclockwise)

Preceding gene: 21673122

Following gene: 21673118

Centisome position: 13.67

GC content: 64.98

Gene sequence:

>1962_bases
ATGAAATCCGTCAACCTCATTATCGATGGCCGCAGCGTGCAGGCGGCTCCGGGACAGACCATCCTCGAAGCGGCCCGCGA
AAACGGCATCCGCATCCCGACGCTCTGCTTCCACAAAGAGATCGAAGCGACCGGTTCGTGCTGGATCTGCATCGTGGAGA
TCAAGGGCAAAAACCGCTTCATCCCCTCGTGCAGCACGAAGGTAAGCGAAGGGATGGTGGTCGAAACGAGCAATGCCGAG
CTCGACGCCATGCGCCGCCAGAGCCTTGAACGGCTGCTTGCCCGGCACTGCGGCGACTGCCTCGCGCCGTGCGAGCTGGC
CTGCCCGGCGGGTTGCGACATTCCCGGATTCGTCTCGGCCATCGCCAAAGGTAACGACCGCGAAGCGCTCGAAATCATCC
GCCGCACCATTCCGCTGCCGGGCATTCTCGGTCGCGTCTGCCCCGCACCGTGCGAGGAGGCGTGCCGCCGCCACGGCGTT
GACGAGCCGGTCTCGATCTGCGCCCTCAAGCGCTTCGCTGCTGATCAGGATATGGTTGAAGGATCGGGACTTCCGGAGCG
CAAACCGGCCTCCGGCAAGCGCGTGGCCATCGTCGGCGCAGGCCCGGCGGGTCTGACCGCCGCTTGGTATCTGCTGCTCG
ACGGGCACGCGGTGACGGTTCTCGATGCGAACGAAAAAGCGGGCGGCATGATGCGTTACGGCATCCCGAAGTTCCGCCTG
CCCGAAGCGGTGATCGACGCCGACGTCAAGCCGCTCGTGAAGATGGGCGCGGAGTTCCGCTTCTCGACCCTGTTCGGCAA
GGACGCAAACCTGGAGGAATTGCAGCAGGAGCACGATGCCGTGCTGCTCACCATCGGCGCGAGCCAGGCATCGAAGCTCG
GCATTCCGGGCGAAGAGCTTGACGGCGTACAGAGCGGCATCGGTTTCCTCGCCAATGTTGCGGACGGAAAAGCTGCCGCG
CCCGGCAAGTCGGTGATCGTCATCGGCGGCGGCAACACAGCCATCGACGCGGCACGAACCGCCCTGCGGCTCGGTGCGGA
ATCGGTGACGATTCTCTACCGCCGCGGACGCGAAGAGATGCCCGCTAACCGGCTGGAAATCGAGGAGGCGGTCGCCGAGG
GCGTCGAGCTTCGCCTGCTGGCCGCGCCGGTCGCGATTGAAAAAGGAGCGAATAGTCTTGTCGTCACGGCGGTCGAAATG
CAGCTCGGCGAACCCGACGCGAGCGGACGCCGCCGCCCGGTTCCCGTCGCGGGATCGGAGTTCACGCTCCACGCCGACAC
GGTCATCTCGGCCACCGGCCAGCAGGTTGACCTCCCGGCTGAAGCGGCAGCGGGTATCGGCGTCGAGCGCAACGGAACAG
TCAAAATCAATGGAGAATCTATGCTCACTGGCGCGGCGGGCGTCTTCGCGGCGGGCGACTGCGTCAGCGGCCCCGACCTC
GCCATCAAAGCTGTCCGGCAGGGTCGCCTCGCCGCTGAAGCGATTGACCGCTACCTCAACGGCGGCGATCCGGCAGCAAC
AGGAATGTCGATGTTCAACTCATCGTACGGAGCGCGAGACAAGGCCCCGCACCAGTTCTACGACCGGGCGAGGCCTGCGG
CACGGGTTGCCGTGCCGGAGCTTGAGGCGGAATCGAGACGCCAGAGCTTCGAGGAGGCGGTAACGGGGTACGATCCGGAA
AAGGCGCGAGAGGAGGCCAAACGCTGCCTGCGATGCCGCTGCCAGGCGGTTGACGATTGCCGCCTGCGGAATCTGGCCAC
GGCGTTCGGCGTGGCAACACCGGCCACCGAAGAGGCCCACGAATACTTCAGCATCGACAGGTCAGGCGACATGCGTTTCG
AACGGGAAAAGTGCATCGATTGCGGCATCTGCATCCGCACGTTGGAGTCGGCTTCGGCAGATGCAGTCACGATCCGGGAA
GAGCTGATCGACCACTGCCCGACCGGAGCGATCAGCAGATAA

Upstream 100 bases:

>100_bases
ATGTGGCTGCTCGAAAAGGTGGCCAACTATATCGACAAGACCTTCGGCGATGGCTCCTCCAACCCGAACGCATGAACGCA
ACCACGAAATGATACCGACC

Downstream 100 bases:

>100_bases
GAGGGTTGATAACTCGCTTTTCTATCGTCAGTTATCCTCTTCTTTCCGAGGATGGTCTTCACTTTTGAAAAGCCAGTCGA
CCGCCTTCATCGCGCCCTCA

Product: putative NADPH-dependent glutamate synthase small subunit

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MKSVNLIIDGRSVQAAPGQTILEAARENGIRIPTLCFHKEIEATGSCWICIVEIKGKNRFIPSCSTKVSEGMVVETSNAE
LDAMRRQSLERLLARHCGDCLAPCELACPAGCDIPGFVSAIAKGNDREALEIIRRTIPLPGILGRVCPAPCEEACRRHGV
DEPVSICALKRFAADQDMVEGSGLPERKPASGKRVAIVGAGPAGLTAAWYLLLDGHAVTVLDANEKAGGMMRYGIPKFRL
PEAVIDADVKPLVKMGAEFRFSTLFGKDANLEELQQEHDAVLLTIGASQASKLGIPGEELDGVQSGIGFLANVADGKAAA
PGKSVIVIGGGNTAIDAARTALRLGAESVTILYRRGREEMPANRLEIEEAVAEGVELRLLAAPVAIEKGANSLVVTAVEM
QLGEPDASGRRRPVPVAGSEFTLHADTVISATGQQVDLPAEAAAGIGVERNGTVKINGESMLTGAAGVFAAGDCVSGPDL
AIKAVRQGRLAAEAIDRYLNGGDPAATGMSMFNSSYGARDKAPHQFYDRARPAARVAVPELEAESRRQSFEEAVTGYDPE
KAREEAKRCLRCRCQAVDDCRLRNLATAFGVATPATEEAHEYFSIDRSGDMRFEREKCIDCGICIRTLESASADAVTIRE
ELIDHCPTGAISR

Sequences:

>Translated_653_residues
MKSVNLIIDGRSVQAAPGQTILEAARENGIRIPTLCFHKEIEATGSCWICIVEIKGKNRFIPSCSTKVSEGMVVETSNAE
LDAMRRQSLERLLARHCGDCLAPCELACPAGCDIPGFVSAIAKGNDREALEIIRRTIPLPGILGRVCPAPCEEACRRHGV
DEPVSICALKRFAADQDMVEGSGLPERKPASGKRVAIVGAGPAGLTAAWYLLLDGHAVTVLDANEKAGGMMRYGIPKFRL
PEAVIDADVKPLVKMGAEFRFSTLFGKDANLEELQQEHDAVLLTIGASQASKLGIPGEELDGVQSGIGFLANVADGKAAA
PGKSVIVIGGGNTAIDAARTALRLGAESVTILYRRGREEMPANRLEIEEAVAEGVELRLLAAPVAIEKGANSLVVTAVEM
QLGEPDASGRRRPVPVAGSEFTLHADTVISATGQQVDLPAEAAAGIGVERNGTVKINGESMLTGAAGVFAAGDCVSGPDL
AIKAVRQGRLAAEAIDRYLNGGDPAATGMSMFNSSYGARDKAPHQFYDRARPAARVAVPELEAESRRQSFEEAVTGYDPE
KAREEAKRCLRCRCQAVDDCRLRNLATAFGVATPATEEAHEYFSIDRSGDMRFEREKCIDCGICIRTLESASADAVTIRE
ELIDHCPTGAISR
>Mature_653_residues
MKSVNLIIDGRSVQAAPGQTILEAARENGIRIPTLCFHKEIEATGSCWICIVEIKGKNRFIPSCSTKVSEGMVVETSNAE
LDAMRRQSLERLLARHCGDCLAPCELACPAGCDIPGFVSAIAKGNDREALEIIRRTIPLPGILGRVCPAPCEEACRRHGV
DEPVSICALKRFAADQDMVEGSGLPERKPASGKRVAIVGAGPAGLTAAWYLLLDGHAVTVLDANEKAGGMMRYGIPKFRL
PEAVIDADVKPLVKMGAEFRFSTLFGKDANLEELQQEHDAVLLTIGASQASKLGIPGEELDGVQSGIGFLANVADGKAAA
PGKSVIVIGGGNTAIDAARTALRLGAESVTILYRRGREEMPANRLEIEEAVAEGVELRLLAAPVAIEKGANSLVVTAVEM
QLGEPDASGRRRPVPVAGSEFTLHADTVISATGQQVDLPAEAAAGIGVERNGTVKINGESMLTGAAGVFAAGDCVSGPDL
AIKAVRQGRLAAEAIDRYLNGGDPAATGMSMFNSSYGARDKAPHQFYDRARPAARVAVPELEAESRRQSFEEAVTGYDPE
KAREEAKRCLRCRCQAVDDCRLRNLATAFGVATPATEEAHEYFSIDRSGDMRFEREKCIDCGICIRTLESASADAVTIRE
ELIDHCPTGAISR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=489, Percent_Identity=29.8568507157464, Blast_Score=156, Evalue=7e-38,
Organism=Homo sapiens, GI33519475, Length=126, Percent_Identity=32.5396825396825, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI111118983, Length=157, Percent_Identity=30.5732484076433, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI111118981, Length=157, Percent_Identity=30.5732484076433, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788811, Length=419, Percent_Identity=37.2315035799523, Blast_Score=249, Evalue=5e-67,
Organism=Escherichia coli, GI1789606, Length=433, Percent_Identity=36.0277136258661, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1789243, Length=538, Percent_Identity=33.6431226765799, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082180, Length=412, Percent_Identity=35.9223300970874, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1788468, Length=409, Percent_Identity=36.4303178484108, Blast_Score=212, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17570289, Length=467, Percent_Identity=31.6916488222698, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71984108, Length=444, Percent_Identity=27.027027027027, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17565758, Length=132, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32566231, Length=132, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17543860, Length=197, Percent_Identity=30.9644670050761, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193209088, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=443, Percent_Identity=29.5711060948081, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28574881, Length=452, Percent_Identity=34.5132743362832, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665539, Length=452, Percent_Identity=34.5132743362832, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665547, Length=452, Percent_Identity=34.5132743362832, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665543, Length=452, Percent_Identity=34.5132743362832, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24640763, Length=495, Percent_Identity=28.6868686868687, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI18858217, Length=495, Percent_Identity=28.6868686868687, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17137178, Length=159, Percent_Identity=35.8490566037736, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640559, Length=137, Percent_Identity=30.6569343065693, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640557, Length=137, Percent_Identity=30.6569343065693, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 69356; Mature: 69356

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVNLIIDGRSVQAAPGQTILEAARENGIRIPTLCFHKEIEATGSCWICIVEIKGKNRF
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCC
IPSCSTKVSEGMVVETSNAELDAMRRQSLERLLARHCGDCLAPCELACPAGCDIPGFVSA
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
IAKGNDREALEIIRRTIPLPGILGRVCPAPCEEACRRHGVDEPVSICALKRFAADQDMVE
HHCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHC
GSGLPERKPASGKRVAIVGAGPAGLTAAWYLLLDGHAVTVLDANEKAGGMMRYGIPKFRL
CCCCCCCCCCCCCEEEEEECCCCHHHHEEHEEECCCEEEEEECCCCCCCEEECCCCCCCC
PEAVIDADVKPLVKMGAEFRFSTLFGKDANLEELQQEHDAVLLTIGASQASKLGIPGEEL
CHHHHCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHH
DGVQSGIGFLANVADGKAAAPGKSVIVIGGGNTAIDAARTALRLGAESVTILYRRGREEM
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
PANRLEIEEAVAEGVELRLLAAPVAIEKGANSLVVTAVEMQLGEPDASGRRRPVPVAGSE
CCHHHHHHHHHHCCCEEEEEECCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
FTLHADTVISATGQQVDLPAEAAAGIGVERNGTVKINGESMLTGAAGVFAAGDCVSGPDL
EEEECCCEEECCCCCCCCCHHHHHCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCCHH
AIKAVRQGRLAAEAIDRYLNGGDPAATGMSMFNSSYGARDKAPHQFYDRARPAARVAVPE
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHEEECCC
LEAESRRQSFEEAVTGYDPEKAREEAKRCLRCRCQAVDDCRLRNLATAFGVATPATEEAH
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
EYFSIDRSGDMRFEREKCIDCGICIRTLESASADAVTIREELIDHCPTGAISR
HHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSVNLIIDGRSVQAAPGQTILEAARENGIRIPTLCFHKEIEATGSCWICIVEIKGKNRF
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCC
IPSCSTKVSEGMVVETSNAELDAMRRQSLERLLARHCGDCLAPCELACPAGCDIPGFVSA
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
IAKGNDREALEIIRRTIPLPGILGRVCPAPCEEACRRHGVDEPVSICALKRFAADQDMVE
HHCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHC
GSGLPERKPASGKRVAIVGAGPAGLTAAWYLLLDGHAVTVLDANEKAGGMMRYGIPKFRL
CCCCCCCCCCCCCEEEEEECCCCHHHHEEHEEECCCEEEEEECCCCCCCEEECCCCCCCC
PEAVIDADVKPLVKMGAEFRFSTLFGKDANLEELQQEHDAVLLTIGASQASKLGIPGEEL
CHHHHCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHH
DGVQSGIGFLANVADGKAAAPGKSVIVIGGGNTAIDAARTALRLGAESVTILYRRGREEM
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
PANRLEIEEAVAEGVELRLLAAPVAIEKGANSLVVTAVEMQLGEPDASGRRRPVPVAGSE
CCHHHHHHHHHHCCCEEEEEECCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
FTLHADTVISATGQQVDLPAEAAAGIGVERNGTVKINGESMLTGAAGVFAAGDCVSGPDL
EEEECCCEEECCCCCCCCCHHHHHCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCCHH
AIKAVRQGRLAAEAIDRYLNGGDPAATGMSMFNSSYGARDKAPHQFYDRARPAARVAVPE
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHEEECCC
LEAESRRQSFEEAVTGYDPEKAREEAKRCLRCRCQAVDDCRLRNLATAFGVATPATEEAH
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
EYFSIDRSGDMRFEREKCIDCGICIRTLESASADAVTIREELIDHCPTGAISR
HHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]