Click here to switch to the map view.

The map label for this gene is ispH [H]

Identifier: 21673122

GI number: 21673122

Start: 294649

End: 295647

Strand: Reverse

Name: ispH [H]

Synonym: CT0283

Alternate gene names: 21673122

Gene position: 295647-294649 (Counterclockwise)

Preceding gene: 21673123

Following gene: 161485731

Centisome position: 13.72

GC content: 59.16

Gene sequence:

>999_bases
GTGAAAATCAATCTTGACAGAACATCGTCCGGCTTCTGCATCGGCGTGCAGGGCACGATTCACGTTGCGGAAGAGAAACT
CGCCCAGTCCGGCGAGCTCTACTGCCTCGGCGATGTGGTGCACAACGAGGTGGAGGTCAAGCGGCTCGAAGCGCTCGGCA
TGGAAACCATCGACATTCCGGCCTTCGAAGAGCTCCGGAATGCCGAGGTACTGATCCGCGCACACGGCGAACCGCCCTCG
ACCTACGAAACCGCACGGAAGAACAATCTCGCGATCACCGATACTACCTGCCCCGTGGTGGCAAAGTTGCAGAGGACCGC
CAAAATGCTGCACCAGCTCGGTTACCAGGTGGTGATCTACGGAAAGAAAGTTCACCCAGAGGTGATCGGCATCAACGGCC
AGTGCGATGACGAAGGGGTCGTCATCAAGCATCCCGACCTGTCGGATCCAGAGGAAATCGCCCCGCTCGACCTCAGCCGC
AAAACAGCACTGATCTCGCAGACCACGATGGACGTGCCCGGTTTTTACGAGCTGAAGAGGAATCTCGAAAAGCTCTTCGC
CGAACACGGCCACCGCAACCCCGGCACACAGAGCGGCGAGTGGATGGCCGTGCGCGACATCGACATCACCGCCGAAAAAA
CCGGCGCTCTCGCGATGCCGAAGCTGGTCTTCAAGGACACTATCTGCCGCCAGGTTTCGAGCCGCAACGGCAAGCTGCGC
GATTTCGCGCTCGCGAACGACTGCATCGTCTTTGCGGCGGGTCGCAAAAGCTCGAACGGCCAGGTGCTCTACTCGATCTG
CAAAGACGCCAATCCACACAGCTACTTCATCGAGGATGTGGATGAAATCCGGCCCGAATGGTTCGTCGGCGAAAACGGCA
AACCGGTCGAAAGCGTCGGCATCTGCGGTGCGACCTCGACCCCCATGTGGCTGCTCGAAAAGGTGGCCAACTATATCGAC
AAGACCTTCGGCGATGGCTCCTCCAACCCGAACGCATGA

Upstream 100 bases:

>100_bases
GCTGGTAGGGATGTAGCCATTGCCTTTCCCATTTTAATCGTATCTTTTCCGGCAAAATCTGAACCTTGAAGGCGACGGCC
TTACCATACCGGAGAGACCA

Downstream 100 bases:

>100_bases
ACGCAACCACGAAATGATACCGACCATGAAATCCGTCAACCTCATTATCGATGGCCGCAGCGTGCAGGCGGCTCCGGGAC
AGACCATCCTCGAAGCGGCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLAQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRNAEVLIRAHGEPPS
TYETARKNNLAITDTTCPVVAKLQRTAKMLHQLGYQVVIYGKKVHPEVIGINGQCDDEGVVIKHPDLSDPEEIAPLDLSR
KTALISQTTMDVPGFYELKRNLEKLFAEHGHRNPGTQSGEWMAVRDIDITAEKTGALAMPKLVFKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIRPEWFVGENGKPVESVGICGATSTPMWLLEKVANYID
KTFGDGSSNPNA

Sequences:

>Translated_332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLAQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRNAEVLIRAHGEPPS
TYETARKNNLAITDTTCPVVAKLQRTAKMLHQLGYQVVIYGKKVHPEVIGINGQCDDEGVVIKHPDLSDPEEIAPLDLSR
KTALISQTTMDVPGFYELKRNLEKLFAEHGHRNPGTQSGEWMAVRDIDITAEKTGALAMPKLVFKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIRPEWFVGENGKPVESVGICGATSTPMWLLEKVANYID
KTFGDGSSNPNA
>Mature_332_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLAQSGELYCLGDVVHNEVEVKRLEALGMETIDIPAFEELRNAEVLIRAHGEPPS
TYETARKNNLAITDTTCPVVAKLQRTAKMLHQLGYQVVIYGKKVHPEVIGINGQCDDEGVVIKHPDLSDPEEIAPLDLSR
KTALISQTTMDVPGFYELKRNLEKLFAEHGHRNPGTQSGEWMAVRDIDITAEKTGALAMPKLVFKDTICRQVSSRNGKLR
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIRPEWFVGENGKPVESVGICGATSTPMWLLEKVANYID
KTFGDGSSNPNA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=327, Percent_Identity=25.9938837920489, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 36573; Mature: 36573

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLAQSGELYCLGDVVHNEVEVKRLEALGMETIDIP
CEEECCCCCCCEEEEECCEEEHHHHHHHCCCCEEEEHHHHHCHHHHHHHHHCCCCEECCC
AFEELRNAEVLIRAHGEPPSTYETARKNNLAITDTTCPVVAKLQRTAKMLHQLGYQVVIY
CHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
GKKVHPEVIGINGQCDDEGVVIKHPDLSDPEEIAPLDLSRKTALISQTTMDVPGFYELKR
CCCCCCEEEECCCCCCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHH
NLEKLFAEHGHRNPGTQSGEWMAVRDIDITAEKTGALAMPKLVFKDTICRQVSSRNGKLR
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIRPEWFVGENGKPVESVG
EEEEECCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHEE
ICGATSTPMWLLEKVANYIDKTFGDGSSNPNA
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLAQSGELYCLGDVVHNEVEVKRLEALGMETIDIP
CEEECCCCCCCEEEEECCEEEHHHHHHHCCCCEEEEHHHHHCHHHHHHHHHCCCCEECCC
AFEELRNAEVLIRAHGEPPSTYETARKNNLAITDTTCPVVAKLQRTAKMLHQLGYQVVIY
CHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
GKKVHPEVIGINGQCDDEGVVIKHPDLSDPEEIAPLDLSRKTALISQTTMDVPGFYELKR
CCCCCCEEEECCCCCCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHH
NLEKLFAEHGHRNPGTQSGEWMAVRDIDITAEKTGALAMPKLVFKDTICRQVSSRNGKLR
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
DFALANDCIVFAAGRKSSNGQVLYSICKDANPHSYFIEDVDEIRPEWFVGENGKPVESVG
EEEEECCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHEE
ICGATSTPMWLLEKVANYIDKTFGDGSSNPNA
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA