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The map label for this gene is hisF

Identifier: 15897513

GI number: 15897513

Start: 522765

End: 523520

Strand: Direct

Name: hisF

Synonym: SSO0597

Alternate gene names: 15897513

Gene position: 522765-523520 (Clockwise)

Preceding gene: 15897512

Following gene: 15897514

Centisome position: 17.47

GC content: 39.29

Gene sequence:

>756_bases
ATGACAACGAAAAGAATAATAGCTTGCCTCGATGTAAAGGACGGTAACGTAGTTAAAGGAGTAAATTTCCTTAATCTCCA
ATTGAAAGGAGATCCGGTTAGCCTAGCCAGTCTATATGAAGAAGAAGGCGCTGATGAGATAGTCTTCTTAGATATAACTG
CAACGATAGAAGCTAGAAAAGCATTATATAATGTCATTAAGGATACTGCGAGCGTATTGTCCATTCCCTTAACAGTTGGT
GGTGGTATTAGAACACCTGACGACGTGTCAATGGCGTTAAGATCTGGAGCAGATAAGGTTAGTATAAATACTGCTGCAGT
TGAAAGTAGCCAAATAGTGAAAAAATCTGCAGAAGAGTTTGGATCACAAGCAGTAGTAGTAGCCATAGATGTTAAGAAGG
TAAGCGGAAATTGGATAGTCTTCACAAAATCCGGAACTTACAATACCAGACTTGATGCTATAAAGTGGGCTAAAAAGGTG
GAAGAACTTGGTGCAGGTGAAATATTGCTAACGAGTATTGATAGAGATGGTACTAGACTTGGTTATGACTTAGAATTAAC
TAGGAAAATAGTTGATTCTGTCAATATCCCAGTAATAGCTAGTGGGGGAGCTGGAAAAATGGAACATTTTTATGAGGTTT
TTTCCCTCGCGAAGGCCGACGCAGCGTTAGCTGCTGGCATATTCCATGATGGAATAATTAAAATAAAAGACTTGAAGTCG
TATTTAAGCCAGAAAGGCATCGAGGTGAGAATGTGA

Upstream 100 bases:

>100_bases
CACACCATATCATAGAGGCTAGTTTTAAGGCCTTAGGATTAGCACTATACGAAGCAACGAGAATAGTAGACAATGAAATA
AGAAGCACGAAGGGGATAAT

Downstream 100 bases:

>100_bases
TTTCCTATAGTCTGCCAAATGAGAGACCGAACGATTTTAGCAGAGTAATCCCAGTAGTCAAGGATATTATTGAGTCTGTT
AAGACCAAGGGAGATAACGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARKALYNVIKDTASVLSIPLTVG
GGIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEFGSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKV
EELGAGEILLTSIDRDGTRLGYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKS
YLSQKGIEVRM

Sequences:

>Translated_251_residues
MTTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARKALYNVIKDTASVLSIPLTVG
GGIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEFGSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKV
EELGAGEILLTSIDRDGTRLGYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKS
YLSQKGIEVRM
>Mature_250_residues
TTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARKALYNVIKDTASVLSIPLTVGG
GIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEFGSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKVE
ELGAGEILLTSIDRDGTRLGYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKSY
LSQKGIEVRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.5252918287938, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=37.6996805111821, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SULSO (O33774)

Other databases:

- EMBL:   U82227
- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   E90206
- RefSeq:   NP_342118.1
- ProteinModelPortal:   O33774
- SMR:   O33774
- GeneID:   1454875
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0597
- NMPDR:   fig|273057.1.peg.541
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   O33774
- ProtClustDB:   PRK02083
- BioCyc:   SSOL273057:SSO0597-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26965; Mature: 26834

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARK
CCCCEEEEEEECCCCCEEECEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEHHHHHH
ALYNVIKDTASVLSIPLTVGGGIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEF
HHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHH
GSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKVEELGAGEILLTSIDRDGTRL
CCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEE
GYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKS
CCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
YLSQKGIEVRM
HHHHCCCEECC
>Mature Secondary Structure 
TTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARK
CCCEEEEEEECCCCCEEECEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEHHHHHH
ALYNVIKDTASVLSIPLTVGGGIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEF
HHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHH
GSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKVEELGAGEILLTSIDRDGTRL
CCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEE
GYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKS
CCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
YLSQKGIEVRM
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9209067; 10701121; 11427726