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The map label for this gene is pulE [H]

Identifier: 16124429

GI number: 16124429

Start: 188347

End: 189822

Strand: Direct

Name: pulE [H]

Synonym: CC_0174

Alternate gene names: 16124429

Gene position: 188347-189822 (Clockwise)

Preceding gene: 16124428

Following gene: 16124430

Centisome position: 4.69

GC content: 69.38

Gene sequence:

>1476_bases
ATGACCACGCCGATGCTCGCCTACGGTTTCGCCAAGCGAAACGGGGTGGTCCTGCTGTCCCTGGGCGAGCGAGCGGTGAT
CGGCGTGCGCGAGGGCTATGACCCGATGGCGCTGGTCGAGATCCGTCGAGCGGTCGGCGCGCCACTGGCGATCGAGCCGA
TGACGGCCCCGGTGTTCGACCGCAAGTTCTCGGAGGTCTATGCGGGCGCGGGCCTGGCCACCGCGTCGAGCGCCGACAGC
CTGGACCTGGAAGGCGGCCTTGGCCTGCTGCTTGAGGATATGCCCGCCGCCGCCGACCTCCTCGACCCGCAGGACGACGC
CCCGGTCATCCGCCTGATCAACGGCCTGATCGCCGAGGCCGTGCGCCTGGGCGCGTCGGACATTCACATCGAGCCCTTCG
AGAAGGCCCTCATCGTCCGGCTTCGGATCGATGGCGTGCTGACCGAGGTGTTGAGCCTGTCGCCGCGCATCGCCGCCATG
CTGGTGTCACGGGTCAAGGTCATGGCCAAGCTGGACATCGCCGAAAAGCGCGTACCGCAGGACGGCCGGATCTCCCTGGC
CCTCGGCGGCAAGAGCCTGGATGTCCGTGTCTCGACCCTGCCGGCCCGCATGGGCGAGCGGGTGGTGATGCGGATCCTCG
ACAAGGACCAGGCGGGCCTGCGTCTGTCCGACCTTGGCATGTCGCCCGATGTGCTGAGCGCGCTGGAGACCGCCGTGCGC
GAGCCGCACGGGATCATCCTGGTCACCGGACCCACGGGCTCGGGCAAGACGACGACGCTCTACGCCGCCCTGGCCGTGTT
GAACGACAACAGCCGCAACATCCTGACCATCGAGGACCCGGTCGAATACGCCGTGCACGGCATCGGCCAGACCCAGGTCA
ACACCAAGGTCGGCATGACCTTCGCCGCGGGCCTGCGCGCCATCCTGCGCCAGGATCCCGACGTCGTCATGGTCGGGGAA
ATCCGCGACTCCGAGACCGCCCAGATCGCCGTCCAGGCCAGCCTGACCGGCCACCTCGTGCTGTCGACGGTTCACACCAA
CGATGCGGCGGGCGCTGTGGTGCGTCTGCGCGACATGGGCGTGGAGCCCTTTCTGCTGGCCTCGACCCTGCGTCTCGTGA
TCGCCCAGCGCCTGGTGCGTCGCCTGTGCGAGCACTGCCGCACGCCCGAGCCGGCGGATGCGGCGACGGCGGCCCTGGTG
GGCCTGCCCGAGGGTGAGATCCTGTATCGCCCCGTCGGCTGCGCCCATTGCGGCCACACCGGCTATCAGGGCCGGATCGG
CGTCTACGAGGCCCTGCGCGTCGACGACCACCTGCGCCGCCTGATCGGCGGTAACGCCAGTGAGCAGGAGATCACCGAGG
CCGGTCTTGATCGCCGTCTGGGGCAGGAGGCGCGGAACTATGTTCTGGCCGGCCTGACGACGGTGGAGGAGGCGCTCCGG
ATCACGGGGCAAGGGGCGCGCGATGGCGACCTTTGA

Upstream 100 bases:

>100_bases
CGCCGGACGGCGACAACTCGCTCGACGCGGTCATGCGCGACTATCTGAACGCGGCGCGTCCGGTCGCCGCGCCGACCGCC
GCGAAGCCGGCGACGACGCC

Downstream 100 bases:

>100_bases
CTATGTGGCCCTGGATCTGTCCGGGCGCACGCGTCGGGGTCAGCTGAAGGCCGTCGACGAGGCCGCCGCAGCCGATCAGT
TGCAGCGCAAGCGCCTGGCG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTTPMLAYGFAKRNGVVLLSLGERAVIGVREGYDPMALVEIRRAVGAPLAIEPMTAPVFDRKFSEVYAGAGLATASSADS
LDLEGGLGLLLEDMPAAADLLDPQDDAPVIRLINGLIAEAVRLGASDIHIEPFEKALIVRLRIDGVLTEVLSLSPRIAAM
LVSRVKVMAKLDIAEKRVPQDGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAGLRLSDLGMSPDVLSALETAVR
EPHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTIEDPVEYAVHGIGQTQVNTKVGMTFAAGLRAILRQDPDVVMVGE
IRDSETAQIAVQASLTGHLVLSTVHTNDAAGAVVRLRDMGVEPFLLASTLRLVIAQRLVRRLCEHCRTPEPADAATAALV
GLPEGEILYRPVGCAHCGHTGYQGRIGVYEALRVDDHLRRLIGGNASEQEITEAGLDRRLGQEARNYVLAGLTTVEEALR
ITGQGARDGDL

Sequences:

>Translated_491_residues
MTTPMLAYGFAKRNGVVLLSLGERAVIGVREGYDPMALVEIRRAVGAPLAIEPMTAPVFDRKFSEVYAGAGLATASSADS
LDLEGGLGLLLEDMPAAADLLDPQDDAPVIRLINGLIAEAVRLGASDIHIEPFEKALIVRLRIDGVLTEVLSLSPRIAAM
LVSRVKVMAKLDIAEKRVPQDGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAGLRLSDLGMSPDVLSALETAVR
EPHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTIEDPVEYAVHGIGQTQVNTKVGMTFAAGLRAILRQDPDVVMVGE
IRDSETAQIAVQASLTGHLVLSTVHTNDAAGAVVRLRDMGVEPFLLASTLRLVIAQRLVRRLCEHCRTPEPADAATAALV
GLPEGEILYRPVGCAHCGHTGYQGRIGVYEALRVDDHLRRLIGGNASEQEITEAGLDRRLGQEARNYVLAGLTTVEEALR
ITGQGARDGDL
>Mature_490_residues
TTPMLAYGFAKRNGVVLLSLGERAVIGVREGYDPMALVEIRRAVGAPLAIEPMTAPVFDRKFSEVYAGAGLATASSADSL
DLEGGLGLLLEDMPAAADLLDPQDDAPVIRLINGLIAEAVRLGASDIHIEPFEKALIVRLRIDGVLTEVLSLSPRIAAML
VSRVKVMAKLDIAEKRVPQDGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAGLRLSDLGMSPDVLSALETAVRE
PHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTIEDPVEYAVHGIGQTQVNTKVGMTFAAGLRAILRQDPDVVMVGEI
RDSETAQIAVQASLTGHLVLSTVHTNDAAGAVVRLRDMGVEPFLLASTLRLVIAQRLVRRLCEHCRTPEPADAATAALVG
LPEGEILYRPVGCAHCGHTGYQGRIGVYEALRVDDHLRRLIGGNASEQEITEAGLDRRLGQEARNYVLAGLTTVEEALRI
TGQGARDGDL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=453, Percent_Identity=52.9801324503311, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786296, Length=408, Percent_Identity=44.3627450980392, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI87082188, Length=275, Percent_Identity=37.4545454545455, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52329; Mature: 52198

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPMLAYGFAKRNGVVLLSLGERAVIGVREGYDPMALVEIRRAVGAPLAIEPMTAPVFD
CCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHH
RKFSEVYAGAGLATASSADSLDLEGGLGLLLEDMPAAADLLDPQDDAPVIRLINGLIAEA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHH
VRLGASDIHIEPFEKALIVRLRIDGVLTEVLSLSPRIAAMLVSRVKVMAKLDIAEKRVPQ
HHCCCCCCEECCCCCEEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAGLRLSDLGMSPDVLSALETAVR
CCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHC
EPHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTIEDPVEYAVHGIGQTQVNTKVGMT
CCCCEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCEECHHHHHH
FAAGLRAILRQDPDVVMVGEIRDSETAQIAVQASLTGHLVLSTVHTNDAAGAVVRLRDMG
HHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCEEEEECCC
VEPFLLASTLRLVIAQRLVRRLCEHCRTPEPADAATAALVGLPEGEILYRPVGCAHCGHT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCCCEEEECCCHHHCCCC
GYQGRIGVYEALRVDDHLRRLIGGNASEQEITEAGLDRRLGQEARNYVLAGLTTVEEALR
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ITGQGARDGDL
HCCCCCCCCCC
>Mature Secondary Structure 
TTPMLAYGFAKRNGVVLLSLGERAVIGVREGYDPMALVEIRRAVGAPLAIEPMTAPVFD
CCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHH
RKFSEVYAGAGLATASSADSLDLEGGLGLLLEDMPAAADLLDPQDDAPVIRLINGLIAEA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHH
VRLGASDIHIEPFEKALIVRLRIDGVLTEVLSLSPRIAAMLVSRVKVMAKLDIAEKRVPQ
HHCCCCCCEECCCCCEEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAGLRLSDLGMSPDVLSALETAVR
CCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHC
EPHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTIEDPVEYAVHGIGQTQVNTKVGMT
CCCCEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCEECHHHHHH
FAAGLRAILRQDPDVVMVGEIRDSETAQIAVQASLTGHLVLSTVHTNDAAGAVVRLRDMG
HHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCEEEEECCC
VEPFLLASTLRLVIAQRLVRRLCEHCRTPEPADAATAALVGLPEGEILYRPVGCAHCGHT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCCCEEEECCCHHHCCCC
GYQGRIGVYEALRVDDHLRRLIGGNASEQEITEAGLDRRLGQEARNYVLAGLTTVEEALR
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ITGQGARDGDL
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]