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The map label for this gene is epsF [H]

Identifier: 16124430

GI number: 16124430

Start: 189809

End: 191020

Strand: Direct

Name: epsF [H]

Synonym: CC_0175

Alternate gene names: 16124430

Gene position: 189809-191020 (Clockwise)

Preceding gene: 16124429

Following gene: 16124431

Centisome position: 4.73

GC content: 68.48

Gene sequence:

>1212_bases
ATGGCGACCTTTGACTATGTGGCCCTGGATCTGTCCGGGCGCACGCGTCGGGGTCAGCTGAAGGCCGTCGACGAGGCCGC
CGCAGCCGATCAGTTGCAGCGCAAGCGCCTGGCGCCGGTCAAGATCATGGCGTCCGTCGCCAAGCCGGCCCGGGTCGGCC
GCTTCGCCCTGGGTCAGGGTCTGAGCGCCAAGGACCGCTCGATGTTCACCCGCCAGCTGGCGACCCTGACGTCGGTCTCG
ACGCTGGAAGAGGCGCTGCGCACGATCGCCCTGCAGGCCGACAAGCCGAAACTGAAGCAGGTGATCGGCGAGGTTCACGC
CGCCGTGCTGGAGGGCTACCGGCTCTCGGACGCCATGGGCCGTCCCGCCAAGGCCTTTCCGCCGCTGTACCGCGCCATGG
TCGCAGCCGGCGAGTCGTCCGGCGCCCTGCCGGCGATCCTGGATCGTCTGGCGGATCTTCTTGAGCAACAGCAGCAGGTC
CGGGCCAAGATCACCACGGCCCTGGTCTATCCGATCGTGCTGGCGGTGGTCGCCTGTCTGGTCGTCGCGGCCCTGATGGT
CTTCGTCATTCCCAAGGTGGTCGAGCAGTTCGACTCGATGGGACAGACCCTGCCGCTCCTGACCCGGCTCGTCATCGGCC
TGTCGACTGGTCTGCAAACCTTCGGCTGGCTGATCGTCGTCAGCGCCGTGGTCGCCGTCTTCGCCTTCCTGCGCGCCCTG
CGCCATCGCCCCTTCAAGCTGGCGGTCGATCGCTGGCTGCTGCGCCTGCCGATGATCGGACGCCTGATCCGGGACGTGCA
CGCCGCGCGCCTGGCCCGTACCCTCGCCACCATGGTCGCCAGCGGCCTGCCGGTGCTGGAGGGCCTGATCCTGACCGCCG
GCACGGTGCGCAACGCTGTGCTGCAAGCCGCGCTCGACGACATGATCGCCGCCATCCGCGAAGGGGGCGCGCTGTCGGTG
GCCATGCGCCGCGCCGAGGTCTTCCCGCCGGTGCTCGTCTACATGACGGCCAGCGGCGAGAGCAGCGGGCGCGTCGAAGC
CATGCTGATGCGCGCGGCCGACTATCTGGAACGCGAGTTCGAGACCTTCACCGCGACGCTGCTGAGCTTGCTGGAACCCG
CTATCATCGTGCTGCTGGGCGGCGTGGTGGCGACGATCGTGCTGTCGATCCTGCTGCCGATCCTGCAGATCAACAATCTC
GCCCTGACCTAG

Upstream 100 bases:

>100_bases
GGTCTTGATCGCCGTCTGGGGCAGGAGGCGCGGAACTATGTTCTGGCCGGCCTGACGACGGTGGAGGAGGCGCTCCGGAT
CACGGGGCAAGGGGCGCGCG

Downstream 100 bases:

>100_bases
GAAGCCAAGCCATGTCGACCGCAAACGCTGAAACGAAACGCCGCCGGGCGCGCCGGGCCGGCTTTACGCTGGTGGAGATG
ATGGTGGTGATCGTCATCCT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MATFDYVALDLSGRTRRGQLKAVDEAAAADQLQRKRLAPVKIMASVAKPARVGRFALGQGLSAKDRSMFTRQLATLTSVS
TLEEALRTIALQADKPKLKQVIGEVHAAVLEGYRLSDAMGRPAKAFPPLYRAMVAAGESSGALPAILDRLADLLEQQQQV
RAKITTALVYPIVLAVVACLVVAALMVFVIPKVVEQFDSMGQTLPLLTRLVIGLSTGLQTFGWLIVVSAVVAVFAFLRAL
RHRPFKLAVDRWLLRLPMIGRLIRDVHAARLARTLATMVASGLPVLEGLILTAGTVRNAVLQAALDDMIAAIREGGALSV
AMRRAEVFPPVLVYMTASGESSGRVEAMLMRAADYLEREFETFTATLLSLLEPAIIVLLGGVVATIVLSILLPILQINNL
ALT

Sequences:

>Translated_403_residues
MATFDYVALDLSGRTRRGQLKAVDEAAAADQLQRKRLAPVKIMASVAKPARVGRFALGQGLSAKDRSMFTRQLATLTSVS
TLEEALRTIALQADKPKLKQVIGEVHAAVLEGYRLSDAMGRPAKAFPPLYRAMVAAGESSGALPAILDRLADLLEQQQQV
RAKITTALVYPIVLAVVACLVVAALMVFVIPKVVEQFDSMGQTLPLLTRLVIGLSTGLQTFGWLIVVSAVVAVFAFLRAL
RHRPFKLAVDRWLLRLPMIGRLIRDVHAARLARTLATMVASGLPVLEGLILTAGTVRNAVLQAALDDMIAAIREGGALSV
AMRRAEVFPPVLVYMTASGESSGRVEAMLMRAADYLEREFETFTATLLSLLEPAIIVLLGGVVATIVLSILLPILQINNL
ALT
>Mature_402_residues
ATFDYVALDLSGRTRRGQLKAVDEAAAADQLQRKRLAPVKIMASVAKPARVGRFALGQGLSAKDRSMFTRQLATLTSVST
LEEALRTIALQADKPKLKQVIGEVHAAVLEGYRLSDAMGRPAKAFPPLYRAMVAAGESSGALPAILDRLADLLEQQQQVR
AKITTALVYPIVLAVVACLVVAALMVFVIPKVVEQFDSMGQTLPLLTRLVIGLSTGLQTFGWLIVVSAVVAVFAFLRALR
HRPFKLAVDRWLLRLPMIGRLIRDVHAARLARTLATMVASGLPVLEGLILTAGTVRNAVLQAALDDMIAAIREGGALSVA
MRRAEVFPPVLVYMTASGESSGRVEAMLMRAADYLEREFETFTATLLSLLEPAIIVLLGGVVATIVLSILLPILQINNLA
LT

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=399, Percent_Identity=35.3383458646617, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1786295, Length=359, Percent_Identity=28.9693593314763, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43376; Mature: 43245

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFDYVALDLSGRTRRGQLKAVDEAAAADQLQRKRLAPVKIMASVAKPARVGRFALGQG
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCC
LSAKDRSMFTRQLATLTSVSTLEEALRTIALQADKPKLKQVIGEVHAAVLEGYRLSDAMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCC
RPAKAFPPLYRAMVAAGESSGALPAILDRLADLLEQQQQVRAKITTALVYPIVLAVVACL
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAALMVFVIPKVVEQFDSMGQTLPLLTRLVIGLSTGLQTFGWLIVVSAVVAVFAFLRAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHRPFKLAVDRWLLRLPMIGRLIRDVHAARLARTLATMVASGLPVLEGLILTAGTVRNAV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LQAALDDMIAAIREGGALSVAMRRAEVFPPVLVYMTASGESSGRVEAMLMRAADYLEREF
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ETFTATLLSLLEPAIIVLLGGVVATIVLSILLPILQINNLALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATFDYVALDLSGRTRRGQLKAVDEAAAADQLQRKRLAPVKIMASVAKPARVGRFALGQG
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCC
LSAKDRSMFTRQLATLTSVSTLEEALRTIALQADKPKLKQVIGEVHAAVLEGYRLSDAMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCC
RPAKAFPPLYRAMVAAGESSGALPAILDRLADLLEQQQQVRAKITTALVYPIVLAVVACL
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAALMVFVIPKVVEQFDSMGQTLPLLTRLVIGLSTGLQTFGWLIVVSAVVAVFAFLRAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHRPFKLAVDRWLLRLPMIGRLIRDVHAARLARTLATMVASGLPVLEGLILTAGTVRNAV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LQAALDDMIAAIREGGALSVAMRRAEVFPPVLVYMTASGESSGRVEAMLMRAADYLEREF
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ETFTATLLSLLEPAIIVLLGGVVATIVLSILLPILQINNLALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]