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The map label for this gene is braE [H]

Identifier: 13488197

GI number: 13488197

Start: 36866

End: 37801

Strand: Direct

Name: braE [H]

Synonym: mlr9036

Alternate gene names: 13488197

Gene position: 36866-37801 (Clockwise)

Preceding gene: 13488196

Following gene: 13488198

Centisome position: 10.48

GC content: 57.16

Gene sequence:

>936_bases
ATGATGGGCGCTCTGCATCGGGACATCGCAACCATCATCGTTGTTGGGCTTGCACTTGTCGTCGGCGGGCTCGTCGGCAC
CCCATACATTCTGGCAGTCTTGGTCATCTTCGGCATGGTCTCAATTCTGGTCTTGTCCTATCGGACTATCACCACGATGG
GTGGCTGGTCGTTCGCCCACGTCGCCCTCATGGGCATAGGGGGCTACTCAGTAGCTATCTTCTCCAAAGCGCCTTTTGAA
ATTCCAGTCCTCGCAGCGGTGGCGCTTGGCGGGGTGTTTGCTGCGTTCTTCGCCGCAATATTGTCTTGGCCTGTTCTTCG
CACCCGCCAGTACTACTTCTTCTTATCGACGTTCGCTGCGGGTGAAGCGCTGCGCCAGTGCTACATCCAAATCAAGGAAG
TGACCGGCGGTACGGCGGGAATTGCTTTCATAGCCCGCCCTGGAGTCCTTCCTGATCCGTCATCAGCGTTTCAGTTTCTA
TGCCTTGTCGCGGTCATCCTCGTATTGCTGGGCCTGTTCTACGCAGCTCTGGACGCGTCCGACACCGGCAAAAAGATCAA
GGCCGTCGGCGAGGACGAGGCCCTGTCGTCTTCGCTCGGTATAGACGCCTGGGCGCTTCGTGCACTCGCCTTCATTCTCG
GCTCGTTTGGGGCTGGCATCGCTGGTGGGCTTTTTGCGTCTTACAACGGCATCATGTCTCCCTCCGACATCAACGCCATG
TTGATGTTCAAGATCGTGGCCGCGTGTGTGATTGGTGGCACCACCCGATTTTCAGGGCCGCTGCTCGGTTTGCTCTTTCT
AACCCTGATTGAAGAGATATTCCGGTTTGTGCCTGAGTTCGTGCCGATGATCTGGGGCATGCTCGTCATCGCCGCCGTCA
TTTTCAGCCAGCGCACCGGCGGAGGCTTCAGGCTTCGTAAGGTGACAAATGCTTGA

Upstream 100 bases:

>100_bases
TGTCGCAGTTCGGCGGCGGGCGCTTTCTTGCTCTGACGAGTGCGGCCATCATGCTGTTCGTCCTTAGCGTAAAACCCTCC
GGTCTATTCGGGGAAAAAGA

Downstream 100 bases:

>100_bases
GGTAAGGGAAATCAGCCGTGCCTTCGGACGCCTCAAGGCAGTTGATGGGGTTACACTTTCGGTAACGAAGAACCAAATCC
GCGGTCTTATCGGCCCCAAC

Product: ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MMGALHRDIATIIVVGLALVVGGLVGTPYILAVLVIFGMVSILVLSYRTITTMGGWSFAHVALMGIGGYSVAIFSKAPFE
IPVLAAVALGGVFAAFFAAILSWPVLRTRQYYFFLSTFAAGEALRQCYIQIKEVTGGTAGIAFIARPGVLPDPSSAFQFL
CLVAVILVLLGLFYAALDASDTGKKIKAVGEDEALSSSLGIDAWALRALAFILGSFGAGIAGGLFASYNGIMSPSDINAM
LMFKIVAACVIGGTTRFSGPLLGLLFLTLIEEIFRFVPEFVPMIWGMLVIAAVIFSQRTGGGFRLRKVTNA

Sequences:

>Translated_311_residues
MMGALHRDIATIIVVGLALVVGGLVGTPYILAVLVIFGMVSILVLSYRTITTMGGWSFAHVALMGIGGYSVAIFSKAPFE
IPVLAAVALGGVFAAFFAAILSWPVLRTRQYYFFLSTFAAGEALRQCYIQIKEVTGGTAGIAFIARPGVLPDPSSAFQFL
CLVAVILVLLGLFYAALDASDTGKKIKAVGEDEALSSSLGIDAWALRALAFILGSFGAGIAGGLFASYNGIMSPSDINAM
LMFKIVAACVIGGTTRFSGPLLGLLFLTLIEEIFRFVPEFVPMIWGMLVIAAVIFSQRTGGGFRLRKVTNA
>Mature_311_residues
MMGALHRDIATIIVVGLALVVGGLVGTPYILAVLVIFGMVSILVLSYRTITTMGGWSFAHVALMGIGGYSVAIFSKAPFE
IPVLAAVALGGVFAAFFAAILSWPVLRTRQYYFFLSTFAAGEALRQCYIQIKEVTGGTAGIAFIARPGVLPDPSSAFQFL
CLVAVILVLLGLFYAALDASDTGKKIKAVGEDEALSSSLGIDAWALRALAFILGSFGAGIAGGLFASYNGIMSPSDINAM
LMFKIVAACVIGGTTRFSGPLLGLLFLTLIEEIFRFVPEFVPMIWGMLVIAAVIFSQRTGGGFRLRKVTNA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=315, Percent_Identity=27.3015873015873, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 32802; Mature: 32802

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGALHRDIATIIVVGLALVVGGLVGTPYILAVLVIFGMVSILVLSYRTITTMGGWSFAH
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VALMGIGGYSVAIFSKAPFEIPVLAAVALGGVFAAFFAAILSWPVLRTRQYYFFLSTFAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
GEALRQCYIQIKEVTGGTAGIAFIARPGVLPDPSSAFQFLCLVAVILVLLGLFYAALDAS
HHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTGKKIKAVGEDEALSSSLGIDAWALRALAFILGSFGAGIAGGLFASYNGIMSPSDINAM
CCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHH
LMFKIVAACVIGGTTRFSGPLLGLLFLTLIEEIFRFVPEFVPMIWGMLVIAAVIFSQRTG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGFRLRKVTNA
CCEEEEEECCC
>Mature Secondary Structure
MMGALHRDIATIIVVGLALVVGGLVGTPYILAVLVIFGMVSILVLSYRTITTMGGWSFAH
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VALMGIGGYSVAIFSKAPFEIPVLAAVALGGVFAAFFAAILSWPVLRTRQYYFFLSTFAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
GEALRQCYIQIKEVTGGTAGIAFIARPGVLPDPSSAFQFLCLVAVILVLLGLFYAALDAS
HHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTGKKIKAVGEDEALSSSLGIDAWALRALAFILGSFGAGIAGGLFASYNGIMSPSDINAM
CCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHH
LMFKIVAACVIGGTTRFSGPLLGLLFLTLIEEIFRFVPEFVPMIWGMLVIAAVIFSQRTG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGFRLRKVTNA
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]