BacMap

Definition	Mesorhizobium loti MAFF303099 plasmid pMLa, complete sequence.
Accession	NC_002679
Length	351,911

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The map label for this gene is braF [H]

Identifier: 13488198

GI number: 13488198

Start: 37794

End: 38552

Strand: Direct

Name: braF [H]

Synonym: mlr9038

Alternate gene names: 13488198

Gene position: 37794-38552 (Clockwise)

Preceding gene: 13488197

Following gene: 13488199

Centisome position: 10.74

GC content: 57.71

Gene sequence:

>759_bases
ATGCTTGAGGTAAGGGAAATCAGCCGTGCCTTCGGACGCCTCAAGGCAGTTGATGGGGTTACACTTTCGGTAACGAAGAA
CCAAATCCGCGGTCTTATCGGCCCCAACGGATCGGGCAAGTCGACGACGATGAACCTAATCAGCGGCAGTCTTAAGCTTT
CCGCTGGAAAAATCGAGCTCGAGGGGCAGCGAATCGAAGGCAAGGAGCAGCACCGAATCGCGCGGCTCGGCCTCATCCGA
ACCTTCCAACTGACCAGGGTATTCACGTCAGGTACTCTCCTAGACGCGGTCGCTCTCGGAGTCCGTGCCGCCAGTGGATC
GCCGTGGAGTCCGGCCCGTTGGATCCCAGCGGGCGATCAGGCCGAAGTCAATCAAGCCGCGTTAGTTGCTTTGGAACGAA
TGGGGTTGGCACATCGCGCCAACGAACTCGCGGGCAATCTTCCTGGCGGTCTGCGACGGATCCTCAGCATCGCGACAGCG
TTGGCAGCCAGGCCAAAGATCATGCTTTTGGACGAGCCGCTGGCTGGGCTGAACGCGACGGAAAAAGCGGAAGTTGCAAA
CAGAATCGAGAAGCTCAGGGCTGACGATGTTTCCATTCTGCTGGTCGAGCATGACATGAGGTCCGTCATGCGTTTGTGCG
ACCGAATTACAGTGCTGAACTTCGGCCAAGTCATTGCAGAAGGTACACCGGCCGAAATCGGAAGGCACCCGGACGTGATC
AAGGCCTACTTGGGTCAGAAGAGGGATCGCGATGCTTGA

Upstream 100 bases:

>100_bases
GTGCCTGAGTTCGTGCCGATGATCTGGGGCATGCTCGTCATCGCCGCCGTCATTTTCAGCCAGCGCACCGGCGGAGGCTT
CAGGCTTCGTAAGGTGACAA

Downstream 100 bases:

>100_bases
AGCGCAAAATATCGTGGTCGAGTATGGCGGCGTGCGCGCGCTGGATGAGCTGAGCTTCTCGGCTCAAATTGGGGACGTCA
CCGCTGTTGTGGGCAGCAAT

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLEVREISRAFGRLKAVDGVTLSVTKNQIRGLIGPNGSGKSTTMNLISGSLKLSAGKIELEGQRIEGKEQHRIARLGLIR
TFQLTRVFTSGTLLDAVALGVRAASGSPWSPARWIPAGDQAEVNQAALVALERMGLAHRANELAGNLPGGLRRILSIATA
LAARPKIMLLDEPLAGLNATEKAEVANRIEKLRADDVSILLVEHDMRSVMRLCDRITVLNFGQVIAEGTPAEIGRHPDVI
KAYLGQKRDRDA

Sequences:

>Translated_252_residues
MLEVREISRAFGRLKAVDGVTLSVTKNQIRGLIGPNGSGKSTTMNLISGSLKLSAGKIELEGQRIEGKEQHRIARLGLIR
TFQLTRVFTSGTLLDAVALGVRAASGSPWSPARWIPAGDQAEVNQAALVALERMGLAHRANELAGNLPGGLRRILSIATA
LAARPKIMLLDEPLAGLNATEKAEVANRIEKLRADDVSILLVEHDMRSVMRLCDRITVLNFGQVIAEGTPAEIGRHPDVI
KAYLGQKRDRDA
>Mature_252_residues
MLEVREISRAFGRLKAVDGVTLSVTKNQIRGLIGPNGSGKSTTMNLISGSLKLSAGKIELEGQRIEGKEQHRIARLGLIR
TFQLTRVFTSGTLLDAVALGVRAASGSPWSPARWIPAGDQAEVNQAALVALERMGLAHRANELAGNLPGGLRRILSIATA
LAARPKIMLLDEPLAGLNATEKAEVANRIEKLRADDVSILLVEHDMRSVMRLCDRITVLNFGQVIAEGTPAEIGRHPDVI
KAYLGQKRDRDA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=215, Percent_Identity=28.3720930232558, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI150417984, Length=235, Percent_Identity=27.2340425531915, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI21536376, Length=226, Percent_Identity=26.1061946902655, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI31657092, Length=234, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI27881501, Length=224, Percent_Identity=29.0178571428571, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI30795238, Length=224, Percent_Identity=29.0178571428571, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI47078218, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI45446740, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116734710, Length=235, Percent_Identity=26.8085106382979, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI11967969, Length=223, Percent_Identity=30.0448430493274, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27262624, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262626, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4557481, Length=219, Percent_Identity=25.1141552511416, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI9955968, Length=219, Percent_Identity=22.8310502283105, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI9955966, Length=219, Percent_Identity=22.8310502283105, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI45243524, Length=219, Percent_Identity=22.8310502283105, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI148612844, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27477115, Length=217, Percent_Identity=25.3456221198157, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI255708477, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27436953, Length=240, Percent_Identity=22.9166666666667, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=41.8326693227092, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789593, Length=245, Percent_Identity=31.0204081632653, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI48994943, Length=233, Percent_Identity=32.1888412017167, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788761, Length=237, Percent_Identity=28.2700421940928, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081709, Length=240, Percent_Identity=29.1666666666667, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787029, Length=238, Percent_Identity=26.890756302521, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789672, Length=253, Percent_Identity=28.4584980237154, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081791, Length=245, Percent_Identity=29.7959183673469, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787712, Length=239, Percent_Identity=28.0334728033473, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789032, Length=234, Percent_Identity=28.6324786324786, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082268, Length=246, Percent_Identity=28.4552845528455, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790739, Length=235, Percent_Identity=31.063829787234, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786398, Length=256, Percent_Identity=27.734375, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787370, Length=244, Percent_Identity=27.4590163934426, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790544, Length=244, Percent_Identity=29.0983606557377, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081782, Length=233, Percent_Identity=29.6137339055794, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790525, Length=244, Percent_Identity=26.2295081967213, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790190, Length=229, Percent_Identity=27.5109170305677, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI48995001, Length=252, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790162, Length=248, Percent_Identity=25.4032258064516, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788472, Length=226, Percent_Identity=26.5486725663717, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788450, Length=250, Percent_Identity=26.8, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787759, Length=256, Percent_Identity=29.296875, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786345, Length=219, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786803, Length=232, Percent_Identity=27.1551724137931, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790467, Length=238, Percent_Identity=26.0504201680672, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788897, Length=212, Percent_Identity=28.3018867924528, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788165, Length=247, Percent_Identity=25.9109311740891, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1786563, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789963, Length=257, Percent_Identity=28.0155642023346, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787500, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786872, Length=235, Percent_Identity=25.531914893617, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788210, Length=245, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789586, Length=241, Percent_Identity=25.7261410788382, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788225, Length=246, Percent_Identity=26.0162601626016, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787792, Length=250, Percent_Identity=23.6, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789991, Length=249, Percent_Identity=26.5060240963855, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081834, Length=217, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789873, Length=229, Percent_Identity=28.3842794759825, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786703, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082267, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787548, Length=255, Percent_Identity=25.0980392156863, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693107, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48994997, Length=239, Percent_Identity=23.8493723849372, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787164, Length=221, Percent_Identity=28.0542986425339, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786253, Length=235, Percent_Identity=25.1063829787234, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788644, Length=249, Percent_Identity=26.5060240963855, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI48994883, Length=249, Percent_Identity=23.2931726907631, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788506, Length=259, Percent_Identity=23.9382239382239, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=212, Percent_Identity=27.8301886792453, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786654, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788053, Length=216, Percent_Identity=28.2407407407407, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789891, Length=214, Percent_Identity=28.0373831775701, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787112, Length=223, Percent_Identity=29.1479820627803, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=233, Percent_Identity=30.4721030042918, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983516, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71983510, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71983523, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI115533608, Length=206, Percent_Identity=29.126213592233, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=227, Percent_Identity=24.6696035242291, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565938, Length=226, Percent_Identity=26.1061946902655, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193202349, Length=223, Percent_Identity=25.5605381165919, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71984940, Length=249, Percent_Identity=25.7028112449799, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=226, Percent_Identity=24.7787610619469, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=215, Percent_Identity=26.046511627907, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=242, Percent_Identity=24.7933884297521, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115535101, Length=227, Percent_Identity=27.3127753303965, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17567269, Length=227, Percent_Identity=25.9911894273128, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71996809, Length=241, Percent_Identity=23.6514522821577, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI193211015, Length=241, Percent_Identity=23.6514522821577, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221512771, Length=228, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574150, Length=218, Percent_Identity=27.5229357798165, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI116007328, Length=218, Percent_Identity=27.5229357798165, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648314, Length=236, Percent_Identity=26.271186440678, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24643674, Length=222, Percent_Identity=22.972972972973, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666092, Length=230, Percent_Identity=25.6521739130435, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573573, Length=254, Percent_Identity=24.0157480314961, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573571, Length=254, Percent_Identity=24.0157480314961, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641565, Length=222, Percent_Identity=27.4774774774775, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27176; Mature: 27176

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVREISRAFGRLKAVDGVTLSVTKNQIRGLIGPNGSGKSTTMNLISGSLKLSAGKIEL
CCCHHHHHHHHHHHHHCCCEEEEEEHHHHCEEECCCCCCCHHHHHHHHCCEEEECCEEEE
EGQRIEGKEQHRIARLGLIRTFQLTRVFTSGTLLDAVALGVRAASGSPWSPARWIPAGDQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AEVNQAALVALERMGLAHRANELAGNLPGGLRRILSIATALAARPKIMLLDEPLAGLNAT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
EKAEVANRIEKLRADDVSILLVEHDMRSVMRLCDRITVLNFGQVIAEGTPAEIGRHPDVI
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
KAYLGQKRDRDA
HHHHCCCCCCCC
>Mature Secondary Structure
MLEVREISRAFGRLKAVDGVTLSVTKNQIRGLIGPNGSGKSTTMNLISGSLKLSAGKIEL
CCCHHHHHHHHHHHHHCCCEEEEEEHHHHCEEECCCCCCCHHHHHHHHCCEEEECCEEEE
EGQRIEGKEQHRIARLGLIRTFQLTRVFTSGTLLDAVALGVRAASGSPWSPARWIPAGDQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AEVNQAALVALERMGLAHRANELAGNLPGGLRRILSIATALAARPKIMLLDEPLAGLNAT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
EKAEVANRIEKLRADDVSILLVEHDMRSVMRLCDRITVLNFGQVIAEGTPAEIGRHPDVI
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
KAYLGQKRDRDA
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]