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The map label for this gene is hisS

Identifier: 13475767

GI number: 13475767

Start: 5695319

End: 5696821

Strand: Direct

Name: hisS

Synonym: mlr6928

Alternate gene names: 13475767

Gene position: 5695319-5696821 (Clockwise)

Preceding gene: 13475764

Following gene: 13475768

Centisome position: 80.94

GC content: 65.34

Gene sequence:

>1503_bases
ATGGCCGACAAATCGGAAAAGACGAAAGCGCGGCTGCCACGCGGTTTTGCCGACCGCAGCGCCGAGGACATCCGCGCCGT
CGAGAAGATGATGGCGACGATCCGCTCGGTCTATGAGCTCTATGGTTTCGAACCCGCCGACCAGCCGCTGATCGAATACA
CCGATGCGCTCGGCAAATTCCTGCCAGACCAGGATCGGCCGAACGAAGGCGTGTTCTCCTTCCAGGATGATGACGAGCAG
TGGCTGTCGCTGCGCTATGACCTGACCGCGCCGACGGCGCGTTTCGTCGCCGAGAACTACGAGCGCCTGCCCAAACCTTA
TCGCAGCTATCGCTCCGGCTGGGTGTTCCGCAACGAGAAGCCAGGCCCCGGCCGCTTCCGCCAGTTCATGCAGTTCGACG
CCGACACGATCGGTACGCCGGGCGTCGCCGCCGACGCCGAGATGGCGATGATGATGGCCGACGTCATGGAAGCGCTGGGC
ATCAAGCGCGGCGACTACGTCATCCGCGTCAACAACCGCAAGGTGCTCGACGGCGTGCTGGAGGCGATCGGCCTTGGCGG
TGAGGAAAACATCGGCCGCCGGCTGACGGTGCTGCGCGCCATCGACAAGCTGGACAAGCTCGGGCCGGAGGGCGTGAAGC
TTCTGCTCGGCCCGGGGCGCTGGGACGGCGGCAAGGAAGGCGAGGGCGACTTCGCCAAGGGCGCCGGCTTGAACAACGGG
CAGGCCGAGGCGGTTCTTCAAGCAACAGCGAGAAATGCGCAGGCCGCCCAGGATTCCGTGGTCGATTCCAACGCGACCTA
TCAGGAAGGCGTCGGCGAACTCGCGACCATCGAGGCCCTGGTCAGGGCGGCTGGATATGGCGAAGACCGCATCGCCATGG
ACCGCTCGGTGGTGCGCGGCCTCGAATATTACACCGGCCCGGTCTTTGAAGCCGAACTCCTGGCCGAAATCCCCAATGAA
GACGGCCAGATCGTGCGCTTTGGTTCGGTCGGCGGCGGTGGGCGCTATGACGGCCTGGTCTCGCGCTTCCGTGGCGAGCC
GGTGCCGGCGACCGGCTTTTCCATCGGCGTGTCGCGGCTAATGACGGCGCTGAAGAACCTCGGCAAGCTCGACAACGCCG
ACGTGATCGCGCCGGTCGTGGTCCTGGTCATGGACAAGGACACCGAAAGCCTCGGCCGCTACCAGAAGATGGTGAGCGAG
TTGCGTGCCGCCGGTATTCGCTCCGAAATGTATCTTGGCGGCGCCGGCATGAAGGCGCAGCTCAAATATGCCGACCGGCG
CGGCTGCCCGGTCGCCATCATCCAGGGCGGCGACGAGCGCGCCAAGGGGGAGCTGCAGATCAAGGATCTGATCGAAGGCG
CACGCATGTCCGCCGAAATCACCGACAACGCCGAATGGCGCGCCGCGCGCCCGGCGCAGGTCACGGTTGCGGAAAGTGAA
CTGGTCGCAGAGGTGAAGAAGATCCTGGCGGCGCAGGCCGAGGAGCGGGCGCGTGGCAAATAG

Upstream 100 bases:

>100_bases
AAACCCTTGCCGCACAAAGCAGCATCGGTTAGGACACGCGCGCTGCGGAAATAGGAAATTTCCTACCGCACATTAGCACG
AAACACCGGGTACCCATCTC

Downstream 100 bases:

>100_bases
AGGTCGCGTTCTGTCGCGCCCCCCTCTGTCCTGCCGGACATCTCCCCCACGAGGGGGGAGATCGGATGTCATCTCGGCTT
TCGCCAATCACCAACCCCTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MADKSEKTKARLPRGFADRSAEDIRAVEKMMATIRSVYELYGFEPADQPLIEYTDALGKFLPDQDRPNEGVFSFQDDDEQ
WLSLRYDLTAPTARFVAENYERLPKPYRSYRSGWVFRNEKPGPGRFRQFMQFDADTIGTPGVAADAEMAMMMADVMEALG
IKRGDYVIRVNNRKVLDGVLEAIGLGGEENIGRRLTVLRAIDKLDKLGPEGVKLLLGPGRWDGGKEGEGDFAKGAGLNNG
QAEAVLQATARNAQAAQDSVVDSNATYQEGVGELATIEALVRAAGYGEDRIAMDRSVVRGLEYYTGPVFEAELLAEIPNE
DGQIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLMTALKNLGKLDNADVIAPVVVLVMDKDTESLGRYQKMVSE
LRAAGIRSEMYLGGAGMKAQLKYADRRGCPVAIIQGGDERAKGELQIKDLIEGARMSAEITDNAEWRAARPAQVTVAESE
LVAEVKKILAAQAEERARGK

Sequences:

>Translated_500_residues
MADKSEKTKARLPRGFADRSAEDIRAVEKMMATIRSVYELYGFEPADQPLIEYTDALGKFLPDQDRPNEGVFSFQDDDEQ
WLSLRYDLTAPTARFVAENYERLPKPYRSYRSGWVFRNEKPGPGRFRQFMQFDADTIGTPGVAADAEMAMMMADVMEALG
IKRGDYVIRVNNRKVLDGVLEAIGLGGEENIGRRLTVLRAIDKLDKLGPEGVKLLLGPGRWDGGKEGEGDFAKGAGLNNG
QAEAVLQATARNAQAAQDSVVDSNATYQEGVGELATIEALVRAAGYGEDRIAMDRSVVRGLEYYTGPVFEAELLAEIPNE
DGQIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLMTALKNLGKLDNADVIAPVVVLVMDKDTESLGRYQKMVSE
LRAAGIRSEMYLGGAGMKAQLKYADRRGCPVAIIQGGDERAKGELQIKDLIEGARMSAEITDNAEWRAARPAQVTVAESE
LVAEVKKILAAQAEERARGK
>Mature_499_residues
ADKSEKTKARLPRGFADRSAEDIRAVEKMMATIRSVYELYGFEPADQPLIEYTDALGKFLPDQDRPNEGVFSFQDDDEQW
LSLRYDLTAPTARFVAENYERLPKPYRSYRSGWVFRNEKPGPGRFRQFMQFDADTIGTPGVAADAEMAMMMADVMEALGI
KRGDYVIRVNNRKVLDGVLEAIGLGGEENIGRRLTVLRAIDKLDKLGPEGVKLLLGPGRWDGGKEGEGDFAKGAGLNNGQ
AEAVLQATARNAQAAQDSVVDSNATYQEGVGELATIEALVRAAGYGEDRIAMDRSVVRGLEYYTGPVFEAELLAEIPNED
GQIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLMTALKNLGKLDNADVIAPVVVLVMDKDTESLGRYQKMVSEL
RAAGIRSEMYLGGAGMKAQLKYADRRGCPVAIIQGGDERAKGELQIKDLIEGARMSAEITDNAEWRAARPAQVTVAESEL
VAEVKKILAAQAEERARGK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=459, Percent_Identity=31.5904139433551, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI15029520, Length=451, Percent_Identity=31.0421286031042, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1788861, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71993686, Length=440, Percent_Identity=30.2272727272727, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71993693, Length=440, Percent_Identity=30.2272727272727, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=30.168776371308, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24643061, Length=491, Percent_Identity=28.7169042769857, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24643059, Length=510, Percent_Identity=27.4509803921569, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_RHILO (Q987T0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107334.1
- ProteinModelPortal:   Q987T0
- SMR:   Q987T0
- GeneID:   1229989
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6928
- NMPDR:   fig|266835.1.peg.5435
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- ProtClustDB:   PRK00037
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 54547; Mature: 54416

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKSEKTKARLPRGFADRSAEDIRAVEKMMATIRSVYELYGFEPADQPLIEYTDALGKF
CCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LPDQDRPNEGVFSFQDDDEQWLSLRYDLTAPTARFVAENYERLPKPYRSYRSGWVFRNEK
CCCCCCCCCCCEECCCCCHHEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCEEECCC
PGPGRFRQFMQFDADTIGTPGVAADAEMAMMMADVMEALGIKRGDYVIRVNNRKVLDGVL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
EAIGLGGEENIGRRLTVLRAIDKLDKLGPEGVKLLLGPGRWDGGKEGEGDFAKGAGLNNG
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QAEAVLQATARNAQAAQDSVVDSNATYQEGVGELATIEALVRAAGYGEDRIAMDRSVVRG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LEYYTGPVFEAELLAEIPNEDGQIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRL
HHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
MTALKNLGKLDNADVIAPVVVLVMDKDTESLGRYQKMVSELRAAGIRSEMYLGGAGMKAQ
HHHHHHCCCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEE
LKYADRRGCPVAIIQGGDERAKGELQIKDLIEGARMSAEITDNAEWRAARPAQVTVAESE
EEECCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHCCEEECCCCCCCCCCCCEEEEHHHH
LVAEVKKILAAQAEERARGK
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADKSEKTKARLPRGFADRSAEDIRAVEKMMATIRSVYELYGFEPADQPLIEYTDALGKF
CCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LPDQDRPNEGVFSFQDDDEQWLSLRYDLTAPTARFVAENYERLPKPYRSYRSGWVFRNEK
CCCCCCCCCCCEECCCCCHHEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCEEECCC
PGPGRFRQFMQFDADTIGTPGVAADAEMAMMMADVMEALGIKRGDYVIRVNNRKVLDGVL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
EAIGLGGEENIGRRLTVLRAIDKLDKLGPEGVKLLLGPGRWDGGKEGEGDFAKGAGLNNG
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QAEAVLQATARNAQAAQDSVVDSNATYQEGVGELATIEALVRAAGYGEDRIAMDRSVVRG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LEYYTGPVFEAELLAEIPNEDGQIVRFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRL
HHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
MTALKNLGKLDNADVIAPVVVLVMDKDTESLGRYQKMVSELRAAGIRSEMYLGGAGMKAQ
HHHHHHCCCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEE
LKYADRRGCPVAIIQGGDERAKGELQIKDLIEGARMSAEITDNAEWRAARPAQVTVAESE
EEECCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHCCEEECCCCCCCCCCCCEEEEHHHH
LVAEVKKILAAQAEERARGK
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968