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The map label for this gene is hisZ

Identifier: 13475768

GI number: 13475768

Start: 5697068

End: 5698189

Strand: Direct

Name: hisZ

Synonym: mlr6929

Alternate gene names: 13475768

Gene position: 5697068-5698189 (Clockwise)

Preceding gene: 13475767

Following gene: 13475769

Centisome position: 80.97

GC content: 68.0

Gene sequence:

>1122_bases
ATGACCTCTCGCTTCCCCGCCATCGCGGCCAACATCACAAAACTCTTCGCCGCGCGCAACACGCATGCGGTCGAGGTCGC
CATCCTGCAGCCGGCGGACCCGTTCCTCGACATGGCCGGGGAGGATTTGCGCCGCCGCATCTTCCTCACCGAGAGTGAGA
CCGGGCAGACACTGTGCCTGCGGCCGGAATTCACCATTCCGGTCTGTCTTGACCATATAAGCTCGCAGGCCGGCACGCCG
CGCCGCTATTCCTATCTCGGGGAGGTGTTTCGCCAGCGCCGCGAAGGCGGCAATGAGTTCTTCCAGGCCGGCATCGAGGA
TCTCGGCGACCGCGACACGGCGCAAGCCGATGCTCGCTCCGTGGCCGACGCCCATGCGCTGCTGTCGCTGGTGCTGCCCG
GCCGGTCGCTGGCGGTCACACTGGGCGACCAGGGCATCTTCGAAGCGGTGCTGGCGGCGCTCGGCCTGCCGCGCGGCTGG
CGTATGCGGCTGGCCCGTGCCTTCGGCTCGGCGCCGATGCTGCAGGCGGCACTTGCCGATCTCGCCAACCCGCCGCGCAA
TGGCCAGCTTTCCGGCGAAGTCGCTGCCCTTGTCCTCGACGGGGATCTTGACGGTCTTTCCACGCACATCGCCGGCGGCA
TGGAACAGGCCGGCCTGTCGGCATCGGCCGGGCGCTCGCCCACAGACATTGCGCGGCGGCTGATCGAGAAGGCCGAATTG
CGCAGCGTGCGGCTGTCGAACGAGGCCTTCGCGGCATTGAAGAATTTTCTGGCGATCCATGTGCCGCTCGATGGTGCGGC
GCGGGCGTTGGAGACCTTTGCCGCCGGGGCCGGGCTGTCGCTGGGTGCCGCACTGGAGAAATTCGCCGCCCGGGCCAAGG
CGATCGAGGCGCATGGCTTGCCGGCGGAAAAAATCCGCTACGATGCCGCCTTCGGCCGTCCACTCGATTATTACACTGGT
GTGGTCTTCGAAATCGCGGCGCAAGGCGGCGAGCGTCCCCTGGCCGGCGGCGGCCGCTACGACCGGCTGCTGACCCTGCT
TGGCGCCAAGACGGCGATCCCCGGCGTCGGCTTTTCCGTCTGGCTCGACCGCATCGAGGCGTTGCGGGAGGCGGCACCAT
GA

Upstream 100 bases:

>100_bases
GAGATGTCCGGCAGGACAGAGGGGGGCGCCGTAGAGCGCCGATCGTTGATCGCCCCCCTGTTCCCAAACCAGAGGGTCGC
GCGATGCAAGGGCGCGCCGC

Downstream 100 bases:

>100_bases
TCACGTTGGCCATTCCCTCGAAAGGCCGGCTCAAGGAACAGGCGCTGGAGGTGCTGGCCAAGGCCGGCCTCGCCATCAGC
CTGCCCGGCGACGAGCGCAA

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTSRFPAIAANITKLFAARNTHAVEVAILQPADPFLDMAGEDLRRRIFLTESETGQTLCLRPEFTIPVCLDHISSQAGTP
RRYSYLGEVFRQRREGGNEFFQAGIEDLGDRDTAQADARSVADAHALLSLVLPGRSLAVTLGDQGIFEAVLAALGLPRGW
RMRLARAFGSAPMLQAALADLANPPRNGQLSGEVAALVLDGDLDGLSTHIAGGMEQAGLSASAGRSPTDIARRLIEKAEL
RSVRLSNEAFAALKNFLAIHVPLDGAARALETFAAGAGLSLGAALEKFAARAKAIEAHGLPAEKIRYDAAFGRPLDYYTG
VVFEIAAQGGERPLAGGGRYDRLLTLLGAKTAIPGVGFSVWLDRIEALREAAP

Sequences:

>Translated_373_residues
MTSRFPAIAANITKLFAARNTHAVEVAILQPADPFLDMAGEDLRRRIFLTESETGQTLCLRPEFTIPVCLDHISSQAGTP
RRYSYLGEVFRQRREGGNEFFQAGIEDLGDRDTAQADARSVADAHALLSLVLPGRSLAVTLGDQGIFEAVLAALGLPRGW
RMRLARAFGSAPMLQAALADLANPPRNGQLSGEVAALVLDGDLDGLSTHIAGGMEQAGLSASAGRSPTDIARRLIEKAEL
RSVRLSNEAFAALKNFLAIHVPLDGAARALETFAAGAGLSLGAALEKFAARAKAIEAHGLPAEKIRYDAAFGRPLDYYTG
VVFEIAAQGGERPLAGGGRYDRLLTLLGAKTAIPGVGFSVWLDRIEALREAAP
>Mature_372_residues
TSRFPAIAANITKLFAARNTHAVEVAILQPADPFLDMAGEDLRRRIFLTESETGQTLCLRPEFTIPVCLDHISSQAGTPR
RYSYLGEVFRQRREGGNEFFQAGIEDLGDRDTAQADARSVADAHALLSLVLPGRSLAVTLGDQGIFEAVLAALGLPRGWR
MRLARAFGSAPMLQAALADLANPPRNGQLSGEVAALVLDGDLDGLSTHIAGGMEQAGLSASAGRSPTDIARRLIEKAELR
SVRLSNEAFAALKNFLAIHVPLDGAARALETFAAGAGLSLGAALEKFAARAKAIEAHGLPAEKIRYDAAFGRPLDYYTGV
VFEIAAQGGERPLAGGGRYDRLLTLLGAKTAIPGVGFSVWLDRIEALREAAP

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_RHILO (Q987S9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107335.1
- ProteinModelPortal:   Q987S9
- GeneID:   1229990
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6929
- NMPDR:   fig|266835.1.peg.5436
- HOGENOM:   HBG334668
- OMA:   PAEPFLD
- ProtClustDB:   PRK12295
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004516
- PANTHER:   PTHR11476

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 39623; Mature: 39491

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRFPAIAANITKLFAARNTHAVEVAILQPADPFLDMAGEDLRRRIFLTESETGQTLCL
CCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCHHHHHEEEEEECCCCCEEEE
RPEFTIPVCLDHISSQAGTPRRYSYLGEVFRQRREGGNEFFQAGIEDLGDRDTAQADARS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
VADAHALLSLVLPGRSLAVTLGDQGIFEAVLAALGLPRGWRMRLARAFGSAPMLQAALAD
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
LANPPRNGQLSGEVAALVLDGDLDGLSTHIAGGMEQAGLSASAGRSPTDIARRLIEKAEL
HCCCCCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RSVRLSNEAFAALKNFLAIHVPLDGAARALETFAAGAGLSLGAALEKFAARAKAIEAHGL
HHHHCCHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PAEKIRYDAAFGRPLDYYTGVVFEIAAQGGERPLAGGGRYDRLLTLLGAKTAIPGVGFSV
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
WLDRIEALREAAP
HHHHHHHHHHCCC
>Mature Secondary Structure 
TSRFPAIAANITKLFAARNTHAVEVAILQPADPFLDMAGEDLRRRIFLTESETGQTLCL
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCHHHHHEEEEEECCCCCEEEE
RPEFTIPVCLDHISSQAGTPRRYSYLGEVFRQRREGGNEFFQAGIEDLGDRDTAQADARS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
VADAHALLSLVLPGRSLAVTLGDQGIFEAVLAALGLPRGWRMRLARAFGSAPMLQAALAD
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
LANPPRNGQLSGEVAALVLDGDLDGLSTHIAGGMEQAGLSASAGRSPTDIARRLIEKAEL
HCCCCCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RSVRLSNEAFAALKNFLAIHVPLDGAARALETFAAGAGLSLGAALEKFAARAKAIEAHGL
HHHHCCHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PAEKIRYDAAFGRPLDYYTGVVFEIAAQGGERPLAGGGRYDRLLTLLGAKTAIPGVGFSV
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
WLDRIEALREAAP
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968