Click here to switch to the map view.

The map label for this gene is nadE

Identifier: 13475627

GI number: 13475627

Start: 5537902

End: 5538861

Strand: Reverse

Name: nadE

Synonym: mll6744

Alternate gene names: 13475627

Gene position: 5538861-5537902 (Counterclockwise)

Preceding gene: 13475628

Following gene: 13475626

Centisome position: 78.72

GC content: 61.56

Gene sequence:

>960_bases
ATGCTGGCGCTCGATGCTCGTGCGGAGGTCGATCGCATCGTTGACACCTTGCGCAAGCAGGTTCTTGGCACGCTCCGCCG
CCGCGGGGTGGTTGTCGGCCTTTCCGGCGGCATCGACAGCTCCGTCGTCGCGACCTTGTGCACACGCGCGTTCGGCAAGG
ACAAGGTCTTGGGACTGTTCATGCCGGAGCGCGATTCCTCCGGCGACTCCCTGAGACTTGGCCGCCGCGTCGCGGCACAG
CTTGGCATTGAGAACATCCTGGAAGACATAGGCCCGGCGGTGGAGGCCGTCGGCGCCTACCGTCGGCAGCTCGAAGCGAT
CCGGACCGTCGTCCCCGACTATGGGGACGGCTGGAAATGCAAGCTGGTGATCGAGCCGGTTCTCGAGAGCAACGGCCTCA
ACATCACCCGGCTCACCGTCCAGGATCCAGAAGGCAAGGTCGATACGGTGCGTCTGTCGCCAGCCGCCTATCTGCAGATC
GTCGCTGCGACCAACTATAAACAGCGCCTGCGCAAGATGACGGAATACTACCACGCCGACCGCCTCAAATACGCGGTTGC
GGGGACGCCAAACCGCCTTGAGTACGACCAGGGGTTTTTTGTCAAGCAGGGCGACGGAACAGCCGACGTGATGCCGATCG
TCCACCTTTACAAAACTCAGGTCTATCAGCTCGCGGAATATCTTGGTGTGGACGAGGAAATCCGCCAGCGACCACCGACC
ACCGACACATTCTCCATGGCGCAAAGCCAGGAGGAGTTCTACTTCGCGCTGCCCTATCATCTGATGGATCTATGCCTCTA
CGGCCTGAACCACGGGATCGCCAGCGATGAGGTCGCGGCGGTTGCTGGCCTCACCGAGCCCCAGCTGCAGAAAGTCTACA
AGGACATCGACGCGAAACGGCGGGCCGCCCACTATCTTCATGCGCGGCCTTTGCTCTCCGTAGAAATTGGCGAGGATTGA

Upstream 100 bases:

>100_bases
GCCCGCTCATCGAGACGGCCGTCGTGCAGACCTATTCCCCGGCCGAGTCTCGCAAAAGGGAGGATCTGTCATCGACGTGA
TCTCACATTTCAGCAAGGAC

Downstream 100 bases:

>100_bases
CCGGGAATGTGCGGCATCGCTGGAATTGTGGCCCTGAACGCCGCGGCGGCTTCTCCTTCACGCGAGGCGCTGATGCGGAT
GGTGGGCACGCTGGCGCATC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLALDARAEVDRIVDTLRKQVLGTLRRRGVVVGLSGGIDSSVVATLCTRAFGKDKVLGLFMPERDSSGDSLRLGRRVAAQ
LGIENILEDIGPAVEAVGAYRRQLEAIRTVVPDYGDGWKCKLVIEPVLESNGLNITRLTVQDPEGKVDTVRLSPAAYLQI
VAATNYKQRLRKMTEYYHADRLKYAVAGTPNRLEYDQGFFVKQGDGTADVMPIVHLYKTQVYQLAEYLGVDEEIRQRPPT
TDTFSMAQSQEEFYFALPYHLMDLCLYGLNHGIASDEVAAVAGLTEPQLQKVYKDIDAKRRAAHYLHARPLLSVEIGED

Sequences:

>Translated_319_residues
MLALDARAEVDRIVDTLRKQVLGTLRRRGVVVGLSGGIDSSVVATLCTRAFGKDKVLGLFMPERDSSGDSLRLGRRVAAQ
LGIENILEDIGPAVEAVGAYRRQLEAIRTVVPDYGDGWKCKLVIEPVLESNGLNITRLTVQDPEGKVDTVRLSPAAYLQI
VAATNYKQRLRKMTEYYHADRLKYAVAGTPNRLEYDQGFFVKQGDGTADVMPIVHLYKTQVYQLAEYLGVDEEIRQRPPT
TDTFSMAQSQEEFYFALPYHLMDLCLYGLNHGIASDEVAAVAGLTEPQLQKVYKDIDAKRRAAHYLHARPLLSVEIGED
>Mature_319_residues
MLALDARAEVDRIVDTLRKQVLGTLRRRGVVVGLSGGIDSSVVATLCTRAFGKDKVLGLFMPERDSSGDSLRLGRRVAAQ
LGIENILEDIGPAVEAVGAYRRQLEAIRTVVPDYGDGWKCKLVIEPVLESNGLNITRLTVQDPEGKVDTVRLSPAAYLQI
VAATNYKQRLRKMTEYYHADRLKYAVAGTPNRLEYDQGFFVKQGDGTADVMPIVHLYKTQVYQLAEYLGVDEEIRQRPPT
TDTFSMAQSQEEFYFALPYHLMDLCLYGLNHGIASDEVAAVAGLTEPQLQKVYKDIDAKRRAAHYLHARPLLSVEIGED

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_RHILO (Q988H0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107194.1
- ProteinModelPortal:   Q988H0
- SMR:   Q988H0
- GeneID:   1229849
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll6744
- NMPDR:   fig|266835.1.peg.5295
- HOGENOM:   HBG351567
- OMA:   HADRLNF
- ProtClustDB:   PRK00876
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 35517; Mature: 35517

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: ACT_SITE 35-35

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALDARAEVDRIVDTLRKQVLGTLRRRGVVVGLSGGIDSSVVATLCTRAFGKDKVLGLF
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
MPERDSSGDSLRLGRRVAAQLGIENILEDIGPAVEAVGAYRRQLEAIRTVVPDYGDGWKC
ECCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
KLVIEPVLESNGLNITRLTVQDPEGKVDTVRLSPAAYLQIVAATNYKQRLRKMTEYYHAD
EEEEEEHHCCCCCEEEEEEEECCCCCEEEEEECHHHHEEEHHHCCHHHHHHHHHHHHHHH
RLKYAVAGTPNRLEYDQGFFVKQGDGTADVMPIVHLYKTQVYQLAEYLGVDEEIRQRPPT
HEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
TDTFSMAQSQEEFYFALPYHLMDLCLYGLNHGIASDEVAAVAGLTEPQLQKVYKDIDAKR
CCHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
RAAHYLHARPLLSVEIGED
HHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MLALDARAEVDRIVDTLRKQVLGTLRRRGVVVGLSGGIDSSVVATLCTRAFGKDKVLGLF
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
MPERDSSGDSLRLGRRVAAQLGIENILEDIGPAVEAVGAYRRQLEAIRTVVPDYGDGWKC
ECCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
KLVIEPVLESNGLNITRLTVQDPEGKVDTVRLSPAAYLQIVAATNYKQRLRKMTEYYHAD
EEEEEEHHCCCCCEEEEEEEECCCCCEEEEEECHHHHEEEHHHCCHHHHHHHHHHHHHHH
RLKYAVAGTPNRLEYDQGFFVKQGDGTADVMPIVHLYKTQVYQLAEYLGVDEEIRQRPPT
HEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
TDTFSMAQSQEEFYFALPYHLMDLCLYGLNHGIASDEVAAVAGLTEPQLQKVYKDIDAKR
CCHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
RAAHYLHARPLLSVEIGED
HHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968