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The map label for this gene is asnB [H]

Identifier: 13475626

GI number: 13475626

Start: 5535868

End: 5537895

Strand: Reverse

Name: asnB [H]

Synonym: mll6743

Alternate gene names: 13475626

Gene position: 5537895-5535868 (Counterclockwise)

Preceding gene: 13475627

Following gene: 13475625

Centisome position: 78.71

GC content: 63.91

Gene sequence:

>2028_bases
ATGTGCGGCATCGCTGGAATTGTGGCCCTGAACGCCGCGGCGGCTTCTCCTTCACGCGAGGCGCTGATGCGGATGGTGGG
CACGCTGGCGCATCGCGGGCCGGACGAGCACGGCCTTTACCGCGACAAGCGCGCGGGGCTGGCGCATGCCCGCCTGTCCG
TCGTCGATCTTTCCAGCGGCCAGCAGCCGCTTGCGGACACAGGCGATACGAGCTGGATCGTGTTCAACGGCGAGATATTC
AACTATGTCGAGCTGCGGGAAAAGCTGATTGCGCTTGGCCATCGCTTCCGAACCCGCAGCGACACGGAGGTCGCGCTGCA
TGCGTATCGCGCGTGGGGCGAAGCGGCCTTCGAGCGGATGAACGGCCAATGGGCAATCGCAATCTGGGACTCAATAGCAG
GTCGCCTCGTCTTGTCGCGCGACCGGTTCGGCATTTGCCCGCTGCATTTTTGTGAGCATGCGGGCCGCCTTTATTTCGCC
AGTGAAGTGAAGGCCATTTTCGCGGCCGATCCAACCATCCCTCGCGCGTTTGATCCTGCCGGCCTCGATCAGACTTTCAC
GCTCTGGACGGTCGTTCCACCGCAAGGGGTATTTCAGGGAATCCACGAGCTCGTACCGGGGCATGTCCGCATCTACCAGG
ATGGCACTGTTCGCGAGCAGGTCTTTTGGCAGCCTTCCTACCCCGAGAATATCGGAGCGCTGCAGGGCGAGTTCACCGGG
TCGTGCGATGACGCGGTGGACCGCGTGCGTGACGCCCTAGAGGCCGCGACGGCACTCCGAATGGTGCGCGCCGACGTGCC
TGTCGGGTGTTACCTCTCGGGAGGACTGGACAGCTCGCTTGTGGCGACACTGGGAAGGCGCTTCGCCGGCCGACGCTTCC
AGACCTTCTCCTTGCGTTTTGCCGACGCCGAATATGACGAGACCCGCTTCCAGCGGCTGGTTGCCGAAGCAACCGGCAGC
GAGCACCATGAGGTGGTGGTTTCGCGCGGCGATATCGCGGCAATCTTTCCTGAGGTGATCCACCACACCGAGCGCCCGAT
ACTGAGGACCGCCCCGGCGCCGCTGTTCCTGCTCTCGAGGCTGGTGCGCGAGCGTGGCATCAAGGTCGTGCTGACCGGCG
AGGGTGCCGACGAAATGTTCGCCGGCTACGACCTGTTCCGCGAAGCCAAGGTCCGCCGATTCTGGGGACGCCAGCCGGCG
TCGACACGACGCTCACGCCTCCTGGAGCGCCTTTACCCGTATCTCACCCGCTCGCCGGTGCAACAGCAAGCGATGGCGCG
CCAGTTCTTCGGGCGCGGGATCGAGGCCTATGACGCGCCTGGCTTTGCCCATGACACACGCTGGCGCAGCACCGGCGCCA
TCAAGCGCCTGTTCTCCGCGGATCTGCGCGCCGAATCCCAACGCCGCGACGCCGTGTCCGAGTTGCTCGCCCGCCTGCCC
GCGGCGTTTTCGCGCTGGAGCCATCTTGCGCAGGACCAGTATCTCGAGGTCCAGACGTTGATGTCGGGCTATCTGCTTTC
CTCGCAGGGCGACAGGATGCTGATGGCTCACTCCGTCGAGGGGCGCTTCCCGTTCCTGGACGATGAACTTGTGAGGCTCG
CGAATTCACTTCCGCCTTCCTACAAGCTGCGTGTCCTCGACGAGAAGCACGTGCTGAAACGCGTGGCGCAGCCGATCGTG
CCCTCCGAGATCGTAACCCGCAAGAAGCAGCCATTCCGAGCGCCCAACGCGCTTTGTTTCGCCGCTGACGCCGCACCGGA
ATACATTCGCGAAGCGCTTTCGGAGACGGCGATACGCAAGGCGAATGTTTTCGATCCGGTTTCGGTCACACGCCTGCTCG
ACAAGTGCCAGGCCAGGACCGGTGACGGTGATCTTTCCAATGCCGACAACATGGCGCTTGTCGGCGTGCTGTCGACCCAG
CTCCTTCACCAGCAGTTTGTCGCGAGCTGTCCCAGTGCAACCCACGTGCGTGGGCTCAGCGTCGACGTCGACCGCGAACA
TCGGGAGGAGCGCTTGCATGTTGCATGA

Upstream 100 bases:

>100_bases
GCAGAAAGTCTACAAGGACATCGACGCGAAACGGCGGGCCGCCCACTATCTTCATGCGCGGCCTTTGCTCTCCGTAGAAA
TTGGCGAGGATTGACCGGGA

Downstream 100 bases:

>100_bases
TGCCGTACCCTTGCTGCATGACTACCTCGCCCACTCCGCCCGCCGGCTCGGCGACAAGGTGGCGCTGGTGTGCGGCAAAC
AACGTGTCACCTATGGCGAG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MCGIAGIVALNAAAASPSREALMRMVGTLAHRGPDEHGLYRDKRAGLAHARLSVVDLSSGQQPLADTGDTSWIVFNGEIF
NYVELREKLIALGHRFRTRSDTEVALHAYRAWGEAAFERMNGQWAIAIWDSIAGRLVLSRDRFGICPLHFCEHAGRLYFA
SEVKAIFAADPTIPRAFDPAGLDQTFTLWTVVPPQGVFQGIHELVPGHVRIYQDGTVREQVFWQPSYPENIGALQGEFTG
SCDDAVDRVRDALEAATALRMVRADVPVGCYLSGGLDSSLVATLGRRFAGRRFQTFSLRFADAEYDETRFQRLVAEATGS
EHHEVVVSRGDIAAIFPEVIHHTERPILRTAPAPLFLLSRLVRERGIKVVLTGEGADEMFAGYDLFREAKVRRFWGRQPA
STRRSRLLERLYPYLTRSPVQQQAMARQFFGRGIEAYDAPGFAHDTRWRSTGAIKRLFSADLRAESQRRDAVSELLARLP
AAFSRWSHLAQDQYLEVQTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDELVRLANSLPPSYKLRVLDEKHVLKRVAQPIV
PSEIVTRKKQPFRAPNALCFAADAAPEYIREALSETAIRKANVFDPVSVTRLLDKCQARTGDGDLSNADNMALVGVLSTQ
LLHQQFVASCPSATHVRGLSVDVDREHREERLHVA

Sequences:

>Translated_675_residues
MCGIAGIVALNAAAASPSREALMRMVGTLAHRGPDEHGLYRDKRAGLAHARLSVVDLSSGQQPLADTGDTSWIVFNGEIF
NYVELREKLIALGHRFRTRSDTEVALHAYRAWGEAAFERMNGQWAIAIWDSIAGRLVLSRDRFGICPLHFCEHAGRLYFA
SEVKAIFAADPTIPRAFDPAGLDQTFTLWTVVPPQGVFQGIHELVPGHVRIYQDGTVREQVFWQPSYPENIGALQGEFTG
SCDDAVDRVRDALEAATALRMVRADVPVGCYLSGGLDSSLVATLGRRFAGRRFQTFSLRFADAEYDETRFQRLVAEATGS
EHHEVVVSRGDIAAIFPEVIHHTERPILRTAPAPLFLLSRLVRERGIKVVLTGEGADEMFAGYDLFREAKVRRFWGRQPA
STRRSRLLERLYPYLTRSPVQQQAMARQFFGRGIEAYDAPGFAHDTRWRSTGAIKRLFSADLRAESQRRDAVSELLARLP
AAFSRWSHLAQDQYLEVQTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDELVRLANSLPPSYKLRVLDEKHVLKRVAQPIV
PSEIVTRKKQPFRAPNALCFAADAAPEYIREALSETAIRKANVFDPVSVTRLLDKCQARTGDGDLSNADNMALVGVLSTQ
LLHQQFVASCPSATHVRGLSVDVDREHREERLHVA
>Mature_675_residues
MCGIAGIVALNAAAASPSREALMRMVGTLAHRGPDEHGLYRDKRAGLAHARLSVVDLSSGQQPLADTGDTSWIVFNGEIF
NYVELREKLIALGHRFRTRSDTEVALHAYRAWGEAAFERMNGQWAIAIWDSIAGRLVLSRDRFGICPLHFCEHAGRLYFA
SEVKAIFAADPTIPRAFDPAGLDQTFTLWTVVPPQGVFQGIHELVPGHVRIYQDGTVREQVFWQPSYPENIGALQGEFTG
SCDDAVDRVRDALEAATALRMVRADVPVGCYLSGGLDSSLVATLGRRFAGRRFQTFSLRFADAEYDETRFQRLVAEATGS
EHHEVVVSRGDIAAIFPEVIHHTERPILRTAPAPLFLLSRLVRERGIKVVLTGEGADEMFAGYDLFREAKVRRFWGRQPA
STRRSRLLERLYPYLTRSPVQQQAMARQFFGRGIEAYDAPGFAHDTRWRSTGAIKRLFSADLRAESQRRDAVSELLARLP
AAFSRWSHLAQDQYLEVQTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDELVRLANSLPPSYKLRVLDEKHVLKRVAQPIV
PSEIVTRKKQPFRAPNALCFAADAAPEYIREALSETAIRKANVFDPVSVTRLLDKCQARTGDGDLSNADNMALVGVLSTQ
LLHQQFVASCPSATHVRGLSVDVDREHREERLHVA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=423, Percent_Identity=26.9503546099291, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229252, Length=423, Percent_Identity=26.9503546099291, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229250, Length=423, Percent_Identity=26.9503546099291, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI296010848, Length=395, Percent_Identity=26.5822784810127, Blast_Score=109, Evalue=1e-23,
Organism=Homo sapiens, GI296010852, Length=324, Percent_Identity=26.2345679012346, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI296010850, Length=324, Percent_Identity=26.2345679012346, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786889, Length=406, Percent_Identity=32.2660098522167, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25147557, Length=401, Percent_Identity=28.1795511221945, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=401, Percent_Identity=28.1795511221945, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25147560, Length=338, Percent_Identity=26.3313609467456, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560178, Length=401, Percent_Identity=26.6832917705736, Blast_Score=89, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321563, Length=425, Percent_Identity=28.2352941176471, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325403, Length=426, Percent_Identity=28.169014084507, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45553209, Length=406, Percent_Identity=30.0492610837438, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 75372; Mature: 75372

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIVALNAAAASPSREALMRMVGTLAHRGPDEHGLYRDKRAGLAHARLSVVDLSSG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHEEEEEECCCC
QQPLADTGDTSWIVFNGEIFNYVELREKLIALGHRFRTRSDTEVALHAYRAWGEAAFERM
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
NGQWAIAIWDSIAGRLVLSRDRFGICPLHFCEHAGRLYFASEVKAIFAADPTIPRAFDPA
CCCEEEEEHHHHHCCEEEECCCCCCCHHHHHHHCCCEEEHHHHHHHEECCCCCCCCCCCC
GLDQTFTLWTVVPPQGVFQGIHELVPGHVRIYQDGTVREQVFWQPSYPENIGALQGEFTG
CCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHEEECCCCCCCCCCCCCCCCC
SCDDAVDRVRDALEAATALRMVRADVPVGCYLSGGLDSSLVATLGRRFAGRRFQTFSLRF
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
ADAEYDETRFQRLVAEATGSEHHEVVVSRGDIAAIFPEVIHHTERPILRTAPAPLFLLSR
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHH
LVRERGIKVVLTGEGADEMFAGYDLFREAKVRRFWGRQPASTRRSRLLERLYPYLTRSPV
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHH
QQQAMARQFFGRGIEAYDAPGFAHDTRWRSTGAIKRLFSADLRAESQRRDAVSELLARLP
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSRWSHLAQDQYLEVQTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDELVRLANSLPPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCC
YKLRVLDEKHVLKRVAQPIVPSEIVTRKKQPFRAPNALCFAADAAPEYIREALSETAIRK
EEEEECCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ANVFDPVSVTRLLDKCQARTGDGDLSNADNMALVGVLSTQLLHQQFVASCPSATHVRGLS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCE
VDVDREHREERLHVA
ECCCHHHHHHHCCCC
>Mature Secondary Structure
MCGIAGIVALNAAAASPSREALMRMVGTLAHRGPDEHGLYRDKRAGLAHARLSVVDLSSG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHEEEEEECCCC
QQPLADTGDTSWIVFNGEIFNYVELREKLIALGHRFRTRSDTEVALHAYRAWGEAAFERM
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
NGQWAIAIWDSIAGRLVLSRDRFGICPLHFCEHAGRLYFASEVKAIFAADPTIPRAFDPA
CCCEEEEEHHHHHCCEEEECCCCCCCHHHHHHHCCCEEEHHHHHHHEECCCCCCCCCCCC
GLDQTFTLWTVVPPQGVFQGIHELVPGHVRIYQDGTVREQVFWQPSYPENIGALQGEFTG
CCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHEEECCCCCCCCCCCCCCCCC
SCDDAVDRVRDALEAATALRMVRADVPVGCYLSGGLDSSLVATLGRRFAGRRFQTFSLRF
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
ADAEYDETRFQRLVAEATGSEHHEVVVSRGDIAAIFPEVIHHTERPILRTAPAPLFLLSR
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHH
LVRERGIKVVLTGEGADEMFAGYDLFREAKVRRFWGRQPASTRRSRLLERLYPYLTRSPV
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHH
QQQAMARQFFGRGIEAYDAPGFAHDTRWRSTGAIKRLFSADLRAESQRRDAVSELLARLP
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSRWSHLAQDQYLEVQTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDELVRLANSLPPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCC
YKLRVLDEKHVLKRVAQPIVPSEIVTRKKQPFRAPNALCFAADAAPEYIREALSETAIRK
EEEEECCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ANVFDPVSVTRLLDKCQARTGDGDLSNADNMALVGVLSTQLLHQQFVASCPSATHVRGLS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCE
VDVDREHREERLHVA
ECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]