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The map label for this gene is traC [H]

Identifier: 13475143

GI number: 13475143

Start: 4978428

End: 4979390

Strand: Direct

Name: traC [H]

Synonym: mlr6154

Alternate gene names: 13475143

Gene position: 4978428-4979390 (Clockwise)

Preceding gene: 13475138

Following gene: 13475144

Centisome position: 70.76

GC content: 65.73

Gene sequence:

>963_bases
ATGCGTGCAAAACACCAGCGTTCCGGCAGCCGAGAGGCTGGCGCGGGCAGGGGCGGGCGCCCGGGCGCCAGCCTTTATCA
GGAAATCACGGACCGCATCATTGCCGAACTGGAGCGCGGCACCGTGCCATGGGTCAAGCCATGGGGCAGCGCAAAGGCCG
ACCTCGGCCTGCCCAGCAACGCGGCCACCGGGCGCCGCTATTCCGGCATCAACATCCTCATCCTGTGGGGCGCCGTCATC
GAGCGCAGCTATCCCGGCCAGAACTGGCTGACCTTCCGGCAGGCGCTTGCCCTTGGCGGCAATGTCAGGAAGGGCGAGCG
CGGCACAACAATCGTGCATGCCGACCGCTTTGTCCCAAAGGGCGAGCAGGAGCGCGCCAAGACAGACGGCGACGAGCCGC
AGGCGGTGCCGTACTTGAAGCGCTTCACCGTCTTCAACGTCGCGCAGTGCGACGGCCTGCCCGAGCACCTTTACGGCAAC
GCCGAACCGCTGCCCGAACGCGAGATCGTCCCGCAGGCCGAGGCACTTATTCGAGCAAGCGGCGCGGATTTCCGCATCGG
CGGCGATCGCGCTTTCTACATGCCGGGCAGTGACTCCATTCAGGTGCCGCCGCAGCCGGCATTCTTTCAGCAGATCGACT
ATTACCGCACCTGCTTCCACGAACTTGGCCACTGGACCGGCCACCCGGCACGGCTGGCGCGCGACCTCTCCGGCGCCTTC
GGTTCCAAGACCTATGCGCGCGAGGAACTGGTTGCCGAAATCACTGCTGCCTTTGTCTGCTCGACCCTCGGCATCGAGCC
GACCGTGCGCCATGCCGACTATATCGGCACATGGCTGAAGGTGCTGCGCGAGGACAATCGCGCCATCTTCCGCGCCGCCA
GCCTTGCCTCCAAGGCCGCCGATTTTCTTCTGGGCTTCAACGCCGAGGCCGAAGGCGCGCAACAAGACGAGATCGCGGCA
TGA

Upstream 100 bases:

>100_bases
GGATGCGGTCCGCCTCCGACGGCGGGCCTTTCCGGTCGGGTCTCGCCGCCCACCCCGCTTCCGGGGAAAGGCGCGGGATT
GAAGAGCGGAGGACCAGACG

Downstream 100 bases:

>100_bases
GCGCGCGCGATCCCGACACCCTGCGGACCGAGCCAACGCCGCAAGGCGAGCAGACGCTCATTCCCGGTGTCCAGCCACCA
CGCGCGACCGCCTCGCCCTT

Product: antirestriction protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRAKHQRSGSREAGAGRGGRPGASLYQEITDRIIAELERGTVPWVKPWGSAKADLGLPSNAATGRRYSGINILILWGAVI
ERSYPGQNWLTFRQALALGGNVRKGERGTTIVHADRFVPKGEQERAKTDGDEPQAVPYLKRFTVFNVAQCDGLPEHLYGN
AEPLPEREIVPQAEALIRASGADFRIGGDRAFYMPGSDSIQVPPQPAFFQQIDYYRTCFHELGHWTGHPARLARDLSGAF
GSKTYAREELVAEITAAFVCSTLGIEPTVRHADYIGTWLKVLREDNRAIFRAASLASKAADFLLGFNAEAEGAQQDEIAA

Sequences:

>Translated_320_residues
MRAKHQRSGSREAGAGRGGRPGASLYQEITDRIIAELERGTVPWVKPWGSAKADLGLPSNAATGRRYSGINILILWGAVI
ERSYPGQNWLTFRQALALGGNVRKGERGTTIVHADRFVPKGEQERAKTDGDEPQAVPYLKRFTVFNVAQCDGLPEHLYGN
AEPLPEREIVPQAEALIRASGADFRIGGDRAFYMPGSDSIQVPPQPAFFQQIDYYRTCFHELGHWTGHPARLARDLSGAF
GSKTYAREELVAEITAAFVCSTLGIEPTVRHADYIGTWLKVLREDNRAIFRAASLASKAADFLLGFNAEAEGAQQDEIAA
>Mature_320_residues
MRAKHQRSGSREAGAGRGGRPGASLYQEITDRIIAELERGTVPWVKPWGSAKADLGLPSNAATGRRYSGINILILWGAVI
ERSYPGQNWLTFRQALALGGNVRKGERGTTIVHADRFVPKGEQERAKTDGDEPQAVPYLKRFTVFNVAQCDGLPEHLYGN
AEPLPEREIVPQAEALIRASGADFRIGGDRAFYMPGSDSIQVPPQPAFFQQIDYYRTCFHELGHWTGHPARLARDLSGAF
GSKTYAREELVAEITAAFVCSTLGIEPTVRHADYIGTWLKVLREDNRAIFRAASLASKAADFLLGFNAEAEGAQQDEIAA

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 35096; Mature: 35096

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAKHQRSGSREAGAGRGGRPGASLYQEITDRIIAELERGTVPWVKPWGSAKADLGLPSN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AATGRRYSGINILILWGAVIERSYPGQNWLTFRQALALGGNVRKGERGTTIVHADRFVPK
CCCCCEECCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
GEQERAKTDGDEPQAVPYLKRFTVFNVAQCDGLPEHLYGNAEPLPEREIVPQAEALIRAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHCC
GADFRIGGDRAFYMPGSDSIQVPPQPAFFQQIDYYRTCFHELGHWTGHPARLARDLSGAF
CCCEEECCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GSKTYAREELVAEITAAFVCSTLGIEPTVRHADYIGTWLKVLREDNRAIFRAASLASKAA
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DFLLGFNAEAEGAQQDEIAA
HHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAKHQRSGSREAGAGRGGRPGASLYQEITDRIIAELERGTVPWVKPWGSAKADLGLPSN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AATGRRYSGINILILWGAVIERSYPGQNWLTFRQALALGGNVRKGERGTTIVHADRFVPK
CCCCCEECCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
GEQERAKTDGDEPQAVPYLKRFTVFNVAQCDGLPEHLYGNAEPLPEREIVPQAEALIRAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHCC
GADFRIGGDRAFYMPGSDSIQVPPQPAFFQQIDYYRTCFHELGHWTGHPARLARDLSGAF
CCCEEECCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GSKTYAREELVAEITAAFVCSTLGIEPTVRHADYIGTWLKVLREDNRAIFRAASLASKAA
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DFLLGFNAEAEGAQQDEIAA
HHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]