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The map label for this gene is insF1 [H]

Identifier: 13475138

GI number: 13475138

Start: 4975380

End: 4976240

Strand: Direct

Name: insF1 [H]

Synonym: mlr6150

Alternate gene names: 13475138

Gene position: 4975380-4976240 (Clockwise)

Preceding gene: 13475137

Following gene: 13475143

Centisome position: 70.71

GC content: 62.6

Gene sequence:

>861_bases
ATGAGGTTCGCTTTCATCGCGAGGCACCGGAACATCTGGCCGGTGGCATGGCTGTGCAGTGCGCTGGATGTGTCCCGCTC
GGGCTTCCATGCCTGGCTCAACCGCAGCCCTAGCGTTCGCTCCCAGCATGACGAGATCCTCGTTTCCAGGATCGACCGAA
GCTTCAAGAGCAGCGACCGCACCTACGGCGCGCGTCGGGTCTGGCACGACGTTCTGGCAGAGGGGCTTTCCTGCGGACTG
CATCGCATCGAACGCCTCATGCGGGAGAACGGCCTCCGGGCACGTCCACGCCGGCGCGGACTGCCGAAGGATACCGGCGA
GCGAGCCGCCGTGTCGGACAACCTCCTCGATCGCGCCTTCGAGGCATCGGCCCCGAACCAGAAGTGGGTCGCCGACTTCA
CCTATATCTGGACTGCGGAAGGATGGATCTACGTTGCCGCCGTCGTCGATCTGTTCTCCCGCCGTGTCGTCGGCTGGGCG
ATGAAGGCCGAGATGACCGCGCAGCTCGTCACCGACGCTCTCATCATGGCGATCTGGAGGAGAGGCAAGCCCGACAGCCT
GCTGCACCACTCCGACCAGGGATCGCAATATACGAGCGAGCAGTTCCAGCGACTGATGGCCGACCACGGCATCACCTGCT
CGATGAGCCGGTCGGGCAACGTCTGGGACAACGCTGCGATGGAGAGCTTTTTCTCGTCGCTCAAGACCGAGCGGACAGCC
CGCAAGGTCTACAGGACGAGAGATGATGCGAGGGCGGATGTGTTCGATTACATCGAGCGCTTCTATAACCCTCGGCGAAG
GCACTCGACGCTGGGCTATCTGAGCCCCGTGGAGTTCGAGGAACAAGCTATGTTAGCTTAA

Upstream 100 bases:

>100_bases
AGCAGCAGGAGATTGACCGGCTGCGTAAGGAAGTCGCGAAGCTGAAGGCGGAGCGCGACATCCTAAAAAAGGCCGCAGCC
TACTTCGCGAGGGAAGTGAC

Downstream 100 bases:

>100_bases
CCTCGTGTCCGAAAAACCGGCAGCAGGCCATTGTTCCAGATTCCGCTGCCCTTTGGAGTGAGATGGTGGATTGCGTCTTC
CAGACCGCCCCAAGAATCGA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRFAFIARHRNIWPVAWLCSALDVSRSGFHAWLNRSPSVRSQHDEILVSRIDRSFKSSDRTYGARRVWHDVLAEGLSCGL
HRIERLMRENGLRARPRRRGLPKDTGERAAVSDNLLDRAFEASAPNQKWVADFTYIWTAEGWIYVAAVVDLFSRRVVGWA
MKAEMTAQLVTDALIMAIWRRGKPDSLLHHSDQGSQYTSEQFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERTA
RKVYRTRDDARADVFDYIERFYNPRRRHSTLGYLSPVEFEEQAMLA

Sequences:

>Translated_286_residues
MRFAFIARHRNIWPVAWLCSALDVSRSGFHAWLNRSPSVRSQHDEILVSRIDRSFKSSDRTYGARRVWHDVLAEGLSCGL
HRIERLMRENGLRARPRRRGLPKDTGERAAVSDNLLDRAFEASAPNQKWVADFTYIWTAEGWIYVAAVVDLFSRRVVGWA
MKAEMTAQLVTDALIMAIWRRGKPDSLLHHSDQGSQYTSEQFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERTA
RKVYRTRDDARADVFDYIERFYNPRRRHSTLGYLSPVEFEEQAMLA
>Mature_286_residues
MRFAFIARHRNIWPVAWLCSALDVSRSGFHAWLNRSPSVRSQHDEILVSRIDRSFKSSDRTYGARRVWHDVLAEGLSCGL
HRIERLMRENGLRARPRRRGLPKDTGERAAVSDNLLDRAFEASAPNQKWVADFTYIWTAEGWIYVAAVVDLFSRRVVGWA
MKAEMTAQLVTDALIMAIWRRGKPDSLLHHSDQGSQYTSEQFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERTA
RKVYRTRDDARADVFDYIERFYNPRRRHSTLGYLSPVEFEEQAMLA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=242, Percent_Identity=35.1239669421488, Blast_Score=148, Evalue=5e-36,
Organism=Escherichia coli, GI1788405, Length=287, Percent_Identity=41.4634146341463, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1787263, Length=287, Percent_Identity=41.4634146341463, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1786753, Length=287, Percent_Identity=41.4634146341463, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1786570, Length=287, Percent_Identity=41.4634146341463, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1786490, Length=287, Percent_Identity=41.4634146341463, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1789981, Length=276, Percent_Identity=30.7971014492754, Blast_Score=138, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33109; Mature: 33109

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAFIARHRNIWPVAWLCSALDVSRSGFHAWLNRSPSVRSQHDEILVSRIDRSFKSSDR
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCH
TYGARRVWHDVLAEGLSCGLHRIERLMRENGLRARPRRRGLPKDTGERAAVSDNLLDRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
EASAPNQKWVADFTYIWTAEGWIYVAAVVDLFSRRVVGWAMKAEMTAQLVTDALIMAIWR
HCCCCCCCCEEHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKPDSLLHHSDQGSQYTSEQFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERTA
CCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RKVYRTRDDARADVFDYIERFYNPRRRHSTLGYLSPVEFEEQAMLA
HHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRFAFIARHRNIWPVAWLCSALDVSRSGFHAWLNRSPSVRSQHDEILVSRIDRSFKSSDR
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCH
TYGARRVWHDVLAEGLSCGLHRIERLMRENGLRARPRRRGLPKDTGERAAVSDNLLDRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
EASAPNQKWVADFTYIWTAEGWIYVAAVVDLFSRRVVGWAMKAEMTAQLVTDALIMAIWR
HCCCCCCCCEEHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKPDSLLHHSDQGSQYTSEQFQRLMADHGITCSMSRSGNVWDNAAMESFFSSLKTERTA
CCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RKVYRTRDDARADVFDYIERFYNPRRRHSTLGYLSPVEFEEQAMLA
HHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]