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The map label for this gene is fabI2 [H]

Identifier: 13474637

GI number: 13474637

Start: 4467985

End: 4468788

Strand: Reverse

Name: fabI2 [H]

Synonym: mll5565

Alternate gene names: 13474637

Gene position: 4468788-4467985 (Counterclockwise)

Preceding gene: 13474638

Following gene: 13474636

Centisome position: 63.51

GC content: 63.31

Gene sequence:

>804_bases
ATGGACGGATTGATGAAGGGCAAGCGCGGGCTTGTCATGGGTGTCGCCAACGATCATTCGATCGCCTGGGGCATCGCCAA
GAAATTGTCCGAACATGGGGCGGAGCTCGCCTTCACCTACCAGGGCGACGCCTTCGGCCGCCGGGTCAAGCCGCTCGCCG
ACAAGCTCGGCGCCTCGCTGGTCGTTCCCTGCGATGTCGAGGACAGCGCTTCGGTCGCCGCCACATTCGAGACGCTCGGC
AAGGAATGGGGCGGACTGGACTTCGTCGTCCACGCCATCGGCTTTTCCGACAAGAATGAGTTGAAGGGCCTCTACGCCGA
CACCAGCCGAGACAATTTCGTCCGCACCATGGTGATCTCCTGCTATTCCTTCACCGAAGTGGCGCGCAACGCCGCGGCAC
TGATGACGGAAGGCGGCTCGATGATCACGCTGACCTATGCCGGTTCGGTCCGCGTCATGCCGAACTACAATGTCATGGGC
GTAGCCAAGGCCGGCCTCGAGGCCAGCGTGCGCTACCTTGCCAACGACTACGGCCCGCGCGGCATCCGGGTGAACGGCAT
CTCGGCAGGCCCGGTGCGCACGCTCGCCGGCGCCGGCATTTCGGACGCCCGTCACATGTTCTCCTACCAGCAGCGCAATT
CGCCGTTGCGCCGCACGGTGACCATCGACGAGGTCGGCGGCTCCGCGCTTTATTTGCTGTCCGACCTTGCCTCCGGCGTC
ACCGGCGAAATCCACTATGTCGATTCCGGCTATCACATCGTCTCGATGCCGACGCTCGACGAGTTGAAGCAAACGGATGG
CTAA

Upstream 100 bases:

>100_bases
GTCCTGTCAAATTCCTTCGGTTTCGGTGGCACCAACGCAACGCTCGTTTTCCAGCGCTATTCCGCATAAGGCCAGCGGTT
TTCTAGGGGGATTGCCTGCC

Downstream 100 bases:

>100_bases
CTGGCCCTGAAACGGGTCAAGGCGGGCAACCGTTTCGGAGAAATGGCTCTTTTCCAGTAAAGAAAATAATCTTCTCCAGT
AAAATTGGATTCATTCGCGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDGLMKGKRGLVMGVANDHSIAWGIAKKLSEHGAELAFTYQGDAFGRRVKPLADKLGASLVVPCDVEDSASVAATFETLG
KEWGGLDFVVHAIGFSDKNELKGLYADTSRDNFVRTMVISCYSFTEVARNAAALMTEGGSMITLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNGISAGPVRTLAGAGISDARHMFSYQQRNSPLRRTVTIDEVGGSALYLLSDLASGV
TGEIHYVDSGYHIVSMPTLDELKQTDG

Sequences:

>Translated_267_residues
MDGLMKGKRGLVMGVANDHSIAWGIAKKLSEHGAELAFTYQGDAFGRRVKPLADKLGASLVVPCDVEDSASVAATFETLG
KEWGGLDFVVHAIGFSDKNELKGLYADTSRDNFVRTMVISCYSFTEVARNAAALMTEGGSMITLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNGISAGPVRTLAGAGISDARHMFSYQQRNSPLRRTVTIDEVGGSALYLLSDLASGV
TGEIHYVDSGYHIVSMPTLDELKQTDG
>Mature_267_residues
MDGLMKGKRGLVMGVANDHSIAWGIAKKLSEHGAELAFTYQGDAFGRRVKPLADKLGASLVVPCDVEDSASVAATFETLG
KEWGGLDFVVHAIGFSDKNELKGLYADTSRDNFVRTMVISCYSFTEVARNAAALMTEGGSMITLTYAGSVRVMPNYNVMG
VAKAGLEASVRYLANDYGPRGIRVNGISAGPVRTLAGAGISDARHMFSYQQRNSPLRRTVTIDEVGGSALYLLSDLASGV
TGEIHYVDSGYHIVSMPTLDELKQTDG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=262, Percent_Identity=49.236641221374, Blast_Score=257, Evalue=5e-70,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=27.027027027027, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=30.0395256916996, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28513; Mature: 28513

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLMKGKRGLVMGVANDHSIAWGIAKKLSEHGAELAFTYQGDAFGRRVKPLADKLGASL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEE
VVPCDVEDSASVAATFETLGKEWGGLDFVVHAIGFSDKNELKGLYADTSRDNFVRTMVIS
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEEECCCCCHHHHHHHHH
CYSFTEVARNAAALMTEGGSMITLTYAGSVRVMPNYNVMGVAKAGLEASVRYLANDYGPR
HHHHHHHHHHHHHEEECCCCEEEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
GIRVNGISAGPVRTLAGAGISDARHMFSYQQRNSPLRRTVTIDEVGGSALYLLSDLASGV
CEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCC
TGEIHYVDSGYHIVSMPTLDELKQTDG
CCEEEEEECCEEEEECCCHHHHHHCCC
>Mature Secondary Structure
MDGLMKGKRGLVMGVANDHSIAWGIAKKLSEHGAELAFTYQGDAFGRRVKPLADKLGASL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEE
VVPCDVEDSASVAATFETLGKEWGGLDFVVHAIGFSDKNELKGLYADTSRDNFVRTMVIS
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEEECCCCCHHHHHHHHH
CYSFTEVARNAAALMTEGGSMITLTYAGSVRVMPNYNVMGVAKAGLEASVRYLANDYGPR
HHHHHHHHHHHHHEEECCCCEEEEEECCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
GIRVNGISAGPVRTLAGAGISDARHMFSYQQRNSPLRRTVTIDEVGGSALYLLSDLASGV
CEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCC
TGEIHYVDSGYHIVSMPTLDELKQTDG
CCEEEEEECCEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]