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The map label for this gene is metK [H]

Identifier: 13474623

GI number: 13474623

Start: 4451377

End: 4452642

Strand: Direct

Name: metK [H]

Synonym: mlr5545

Alternate gene names: 13474623

Gene position: 4451377-4452642 (Clockwise)

Preceding gene: 13474622

Following gene: 13474624

Centisome position: 63.27

GC content: 64.85

Gene sequence:

>1266_bases
GTGACGCGGCAGAACTACTTCTTTACCTCGGAATCCGTTGCCGAGGGCCATCCCGACAAAGTCTGCGACCGGATCTCCGA
CGAGATTGTCGATCTGGTCTACCGCGAAGCCAAGAAGACCGGCATGGACCCATGGAAGGTCCGTGTCGCCTGCGAGACCT
TGGCGACCACCAACCGCGTCGTCATCGCCGGCGAAGTGCGCGTCCCCGAGACGCTGCTGAAAAAGGACAAGGCCGGCAAC
ATCCTCAAGGACGCCGCGGGCCACCCGGTCGTGAACCCGGCAAAATTCAAATCCGTCGCCCGCAAGGCGATCCGCGAGAT
CGGCTACGAGCAGGCCGGCTTCCACTGGAAGACGGCCAAGATCGAGGTTCTCCTGCATGGCCAGTCGCCAGACATCGGCC
AGGGCGTCGACAATGCCGCCGACCGCCAGGGCGAGGAAGGGGCCGGCGACCAGGGCATCATGTTCGGCTATGCCTGCCGC
GAGACGCCGGACCTGATGCCGGCGCCGATCTACTACAGCCACAAGATCCTCGAACTTCTGGCCGCCGCCCGCCATCAAAA
CAACGGCGAGGCCGGCAAGCTCGGCCCGGACGCCAAGAGCCAGGTCACCGTCCGCTACGTCGACGGCAAGGCCGCCGAGG
CAACGCAGATCGTGCTGTCGACCCAGCATCTCGACGCGACCTGGGACTCCAAGAAGGTCCGCAAGGTCGTCGAACCCTAT
ATCCGCGAGGCGCTTGGTGATCTCAAGATCGCCGACGATTGCATGTGGTACATCAACCCGACCGGCAAGTTCGTCATCGG
CGGCCCGGACGGCGACGCCGGCCTCACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCGCGGCACCGCATGGCGGAG
GCGCCTTCTCCGGCAAGGACACCACCAAGGTGGACCGTTCGGCAGCCTATGCGGCGCGCTATCTGGCCAAGAATGTCGTG
GCGGCGAAGCTTGCCGACCGCTGCACCATCCAGCTTTCCTACGCCATCGGCGTCGCCCAGCCTCTGTCGGTGTATGTCGA
CCTGCACGGCACCGGCAAGGTCGACGAGGCAAAGCTCGAGGACGCGCTGCGCACGGTGATGGACTTGTCGCCGTCGGGCA
TCCGGCGCCATCTCGACCTCAACAAGCCGATCTACGCTAAGACGTCGTCCTACGGCCATTTCGGCCGCAAGGCCGGCCGT
GACGGGTCTTTCTCCTGGGAAAAGACCGATCTCGCCAAGGCGCTCAAGGATGCTGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
CCAGAATGGCGGCAACGCTTGACGAGCGGCGCTTTGCCCCGCTAACACGTGGCGCGCCAAAATCGGCAGCCTGCCGATCG
TTTTTCAAGAGGGGACACCC

Downstream 100 bases:

>100_bases
GCCGCGACAGACCCAGCGCCGCCAGATGAGCCCGCAAGACAGGCCAAGCCGGACGACCGAAGCCTTTTTCGGTCGTCGGC
GCGGCAAGCCGGTCCGTCCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTRQNYFFTSESVAEGHPDKVCDRISDEIVDLVYREAKKTGMDPWKVRVACETLATTNRVVIAGEVRVPETLLKKDKAGN
ILKDAAGHPVVNPAKFKSVARKAIREIGYEQAGFHWKTAKIEVLLHGQSPDIGQGVDNAADRQGEEGAGDQGIMFGYACR
ETPDLMPAPIYYSHKILELLAAARHQNNGEAGKLGPDAKSQVTVRYVDGKAAEATQIVLSTQHLDATWDSKKVRKVVEPY
IREALGDLKIADDCMWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVV
AAKLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVDEAKLEDALRTVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGR
DGSFSWEKTDLAKALKDAVAA

Sequences:

>Translated_421_residues
MTRQNYFFTSESVAEGHPDKVCDRISDEIVDLVYREAKKTGMDPWKVRVACETLATTNRVVIAGEVRVPETLLKKDKAGN
ILKDAAGHPVVNPAKFKSVARKAIREIGYEQAGFHWKTAKIEVLLHGQSPDIGQGVDNAADRQGEEGAGDQGIMFGYACR
ETPDLMPAPIYYSHKILELLAAARHQNNGEAGKLGPDAKSQVTVRYVDGKAAEATQIVLSTQHLDATWDSKKVRKVVEPY
IREALGDLKIADDCMWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVV
AAKLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVDEAKLEDALRTVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGR
DGSFSWEKTDLAKALKDAVAA
>Mature_420_residues
TRQNYFFTSESVAEGHPDKVCDRISDEIVDLVYREAKKTGMDPWKVRVACETLATTNRVVIAGEVRVPETLLKKDKAGNI
LKDAAGHPVVNPAKFKSVARKAIREIGYEQAGFHWKTAKIEVLLHGQSPDIGQGVDNAADRQGEEGAGDQGIMFGYACRE
TPDLMPAPIYYSHKILELLAAARHQNNGEAGKLGPDAKSQVTVRYVDGKAAEATQIVLSTQHLDATWDSKKVRKVVEPYI
REALGDLKIADDCMWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVA
AKLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVDEAKLEDALRTVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRD
GSFSWEKTDLAKALKDAVAA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=415, Percent_Identity=45.7831325301205, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI4557737, Length=409, Percent_Identity=44.7432762836186, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1789311, Length=416, Percent_Identity=49.2788461538462, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17538494, Length=415, Percent_Identity=43.3734939759036, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17538498, Length=427, Percent_Identity=42.8571428571429, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25145633, Length=423, Percent_Identity=42.789598108747, Blast_Score=287, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17551082, Length=418, Percent_Identity=42.822966507177, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32565911, Length=332, Percent_Identity=45.7831325301205, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25145635, Length=328, Percent_Identity=45.7317073170732, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI32565905, Length=302, Percent_Identity=47.3509933774834, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=47.5083056478405, Blast_Score=231, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6323209, Length=410, Percent_Identity=42.9268292682927, Blast_Score=292, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6320710, Length=415, Percent_Identity=42.4096385542169, Blast_Score=290, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24580527, Length=411, Percent_Identity=44.0389294403893, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24580525, Length=411, Percent_Identity=44.0389294403893, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI45552159, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580529, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580537, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580533, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580535, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580531, Length=411, Percent_Identity=44.0389294403893, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580541, Length=398, Percent_Identity=42.964824120603, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24580539, Length=149, Percent_Identity=37.5838926174497, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45614; Mature: 45483

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQNYFFTSESVAEGHPDKVCDRISDEIVDLVYREAKKTGMDPWKVRVACETLATTNRV
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCEE
VIAGEVRVPETLLKKDKAGNILKDAAGHPVVNPAKFKSVARKAIREIGYEQAGFHWKTAK
EEEECCCCCHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCEEEEEE
IEVLLHGQSPDIGQGVDNAADRQGEEGAGDQGIMFGYACRETPDLMPAPIYYSHKILELL
EEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
AAARHQNNGEAGKLGPDAKSQVTVRYVDGKAAEATQIVLSTQHLDATWDSKKVRKVVEPY
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IREALGDLKIADDCMWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHCCCEEECCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNVVAAKLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVDEAKLE
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHH
DALRTVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRDGSFSWEKTDLAKALKDAVA
HHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
TRQNYFFTSESVAEGHPDKVCDRISDEIVDLVYREAKKTGMDPWKVRVACETLATTNRV
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCEE
VIAGEVRVPETLLKKDKAGNILKDAAGHPVVNPAKFKSVARKAIREIGYEQAGFHWKTAK
EEEECCCCCHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCEEEEEE
IEVLLHGQSPDIGQGVDNAADRQGEEGAGDQGIMFGYACRETPDLMPAPIYYSHKILELL
EEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
AAARHQNNGEAGKLGPDAKSQVTVRYVDGKAAEATQIVLSTQHLDATWDSKKVRKVVEPY
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IREALGDLKIADDCMWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHCCCEEECCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNVVAAKLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVDEAKLE
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHH
DALRTVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRDGSFSWEKTDLAKALKDAVA
HHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA