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The map label for this gene is trmB [H]

Identifier: 13474624

GI number: 13474624

Start: 4452626

End: 4453369

Strand: Direct

Name: trmB [H]

Synonym: mlr5547

Alternate gene names: 13474624

Gene position: 4452626-4453369 (Clockwise)

Preceding gene: 13474623

Following gene: 13474626

Centisome position: 63.28

GC content: 65.19

Gene sequence:

>744_bases
ATGCTGTCGCGGCCTGAGCCGCGACAGACCCAGCGCCGCCAGATGAGCCCGCAAGACAGGCCAAGCCGGACGACCGAAGC
CTTTTTCGGTCGTCGGCGCGGCAAGCCGGTCCGTCCTCAGCAGGCGGCAGCCCTGGAAAGCGGGCTTGACGCCTACCGGC
TCGATCTGACGGCCGATGCGCCGTCGGATCTGCGCACTCTGTTCGAGACTGAAGTTTCGGCCGTCCGGCTCGAGATCGGC
TTTGGCGGCGGCGAGCATCTTCTGCACCGCGCCATCGAGGCGCCGACAACAGGCTTCATCGGCGTCGAGCCCTTCGTCAA
CGGCATGGCCAAGATGATGATGGCGGTGCGAGCAAGGCCGCTGGCCAATTTGCGGGTCCATGACGACGACGCCACCCGGC
TGCTCGACTGGCTGCCGCCGGCTTCGCTTGGCGGCATCGATCTTCTCTATCCAGACCCCTGGCCGAAGAAGAAGCACTGG
AAGCGGCGTTTCGTCAGTCCGGTCAATCTCGAGCGTTTCGCCCGCGTCCTGAAACCGGGCGCAAAATTCCGCTTCGCGTC
CGATATCGACACCTATGTGAACTGGACCTTGCTGCATTGCCGGGCGCATGGCGCCTTCGCATGGCAGGCGGCCGAAGCTG
CCGACTGGCACCGCCCCTATGAGGGCTGGCCTGGCACGCGTTACGAGGCGAAGGCCATTCGCGAGGGCCGGCGGCCTGCC
TATCTGACCTTTGTCAGGAAATAG

Upstream 100 bases:

>100_bases
ATCTACGCTAAGACGTCGTCCTACGGCCATTTCGGCCGCAAGGCCGGCCGTGACGGGTCTTTCTCCTGGGAAAAGACCGA
TCTCGCCAAGGCGCTCAAGG

Downstream 100 bases:

>100_bases
GCCCACCCGCCCTCGCCAGATTTTCAGAAACGGCCGATTCTGTTGAAGATGGCCGGATCCATGCCGAGCTTGCGCAAATC
GTCGGCGCGCGGCTTGCGGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLSRPEPRQTQRRQMSPQDRPSRTTEAFFGRRRGKPVRPQQAAALESGLDAYRLDLTADAPSDLRTLFETEVSAVRLEIG
FGGGEHLLHRAIEAPTTGFIGVEPFVNGMAKMMMAVRARPLANLRVHDDDATRLLDWLPPASLGGIDLLYPDPWPKKKHW
KRRFVSPVNLERFARVLKPGAKFRFASDIDTYVNWTLLHCRAHGAFAWQAAEAADWHRPYEGWPGTRYEAKAIREGRRPA
YLTFVRK

Sequences:

>Translated_247_residues
MLSRPEPRQTQRRQMSPQDRPSRTTEAFFGRRRGKPVRPQQAAALESGLDAYRLDLTADAPSDLRTLFETEVSAVRLEIG
FGGGEHLLHRAIEAPTTGFIGVEPFVNGMAKMMMAVRARPLANLRVHDDDATRLLDWLPPASLGGIDLLYPDPWPKKKHW
KRRFVSPVNLERFARVLKPGAKFRFASDIDTYVNWTLLHCRAHGAFAWQAAEAADWHRPYEGWPGTRYEAKAIREGRRPA
YLTFVRK
>Mature_247_residues
MLSRPEPRQTQRRQMSPQDRPSRTTEAFFGRRRGKPVRPQQAAALESGLDAYRLDLTADAPSDLRTLFETEVSAVRLEIG
FGGGEHLLHRAIEAPTTGFIGVEPFVNGMAKMMMAVRARPLANLRVHDDDATRLLDWLPPASLGGIDLLYPDPWPKKKHW
KRRFVSPVNLERFARVLKPGAKFRFASDIDTYVNWTLLHCRAHGAFAWQAAEAADWHRPYEGWPGTRYEAKAIREGRRPA
YLTFVRK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=228, Percent_Identity=31.140350877193, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 28200; Mature: 28200

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRPEPRQTQRRQMSPQDRPSRTTEAFFGRRRGKPVRPQQAAALESGLDAYRLDLTADA
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCC
PSDLRTLFETEVSAVRLEIGFGGGEHLLHRAIEAPTTGFIGVEPFVNGMAKMMMAVRARP
CHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCC
LANLRVHDDDATRLLDWLPPASLGGIDLLYPDPWPKKKHWKRRFVSPVNLERFARVLKPG
CCCCEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHCCC
AKFRFASDIDTYVNWTLLHCRAHGAFAWQAAEAADWHRPYEGWPGTRYEAKAIREGRRPA
CCEEECCCHHHHHEEEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCE
YLTFVRK
EEEEECC
>Mature Secondary Structure
MLSRPEPRQTQRRQMSPQDRPSRTTEAFFGRRRGKPVRPQQAAALESGLDAYRLDLTADA
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCC
PSDLRTLFETEVSAVRLEIGFGGGEHLLHRAIEAPTTGFIGVEPFVNGMAKMMMAVRARP
CHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCC
LANLRVHDDDATRLLDWLPPASLGGIDLLYPDPWPKKKHWKRRFVSPVNLERFARVLKPG
CCCCEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHCCC
AKFRFASDIDTYVNWTLLHCRAHGAFAWQAAEAADWHRPYEGWPGTRYEAKAIREGRRPA
CCEEECCCHHHHHEEEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCE
YLTFVRK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]