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The map label for this gene is trpA

Identifier: 13474231

GI number: 13474231

Start: 4016001

End: 4016840

Strand: Direct

Name: trpA

Synonym: mlr5073

Alternate gene names: 13474231

Gene position: 4016001-4016840 (Clockwise)

Preceding gene: 13474230

Following gene: 13474232

Centisome position: 57.08

GC content: 65.6

Gene sequence:

>840_bases
ATGACGACCCGCATCGACCGCCGCATGGCGAAGCTGAAGACCGAAGGCCGCCCGGCACTCGTCACCTATTTCATGGGCGG
CGATCCCGACTACGATACCTCGCTGTCGATCATGAAGGCGCTCCCCGGCGCCGGCTCCGACATCATCGAGCTCGGCATGC
CGTTCTCCGATCCGATGGCCGACGGTCCGGCGATCCAGGCGGCAGGGCTGCGGGCGCTGAAAGGCGGCCAGACGCTGGTC
AAGACGCTGAAGATGGCGTCCGAGTTCCGCGCCGGCGACAATGAAACGCCGATCGTGCTGATGGGCTACTACAACCCGAT
CTACATCTATGGCGTCGACCGCTTCCTGAAAGACGCGCTGGCCAGCGGCATCGACGGGCTGATCGTCGTCGACCTGCCGC
CGGAAATGGATGAGGAACTCTGCATTCCGGCGTTGAAGGCCGGCATCAACTTCATCCGCCTGGCGACGCCGACCACCGAC
GACAAGCGCCTGCCCAAGGTGCTGCAGAACACCTCCGGCTTCGTTTACTACGTCTCGATGACCGGCATCACCGGTTCGGC
GCTGGCCGACACCGGCAAGGTGGCGGCGGCAGTCAACCGCATCAAGGGTCACACAGACCTGCCGGTCTGCGTCGGCTTCG
GCGTCAAGACCGCCGAACAGGCGCGTGTCATCGGCGCCAACGCCGATGGCGTTGTCGTCGGCACGGCAATCGTCAATGCG
GTCGCCAATGTGTTGGGGCCGAAGGGCGAAAAGACCGCCGACCCGGCCGAGGCCGTCGCCACACTGGTCAGCGGCCTGGC
GCAAGGCGTGCGTTCGGCCCGCCTTGCTGCTGCCGAATAG

Upstream 100 bases:

>100_bases
TGCACACGGTGGCCTCGATGCTGGGCATGGAGATCTAGGCCCTTTGGACAGTTTCGATTTGGGAGTTGATTATGAGCAAA
ATTCGTCCAAAGGGCCAGAC

Downstream 100 bases:

>100_bases
TTTTCCTACGTCTTTAGCCAACTCTTCGTCAGGACAGGAGCCGAAGCGATGAACTGGATCACCAATTACGTTCGCCCGAA
GATCAATTCGATGCTCGGCC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTLV
KTLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDALASGIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTD
DKRLPKVLQNTSGFVYYVSMTGITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNA
VANVLGPKGEKTADPAEAVATLVSGLAQGVRSARLAAAE

Sequences:

>Translated_279_residues
MTTRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTLV
KTLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDALASGIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTD
DKRLPKVLQNTSGFVYYVSMTGITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNA
VANVLGPKGEKTADPAEAVATLVSGLAQGVRSARLAAAE
>Mature_278_residues
TTRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTLVK
TLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDALASGIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTDD
KRLPKVLQNTSGFVYYVSMTGITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNAV
ANVLGPKGEKTADPAEAVATLVSGLAQGVRSARLAAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=219, Percent_Identity=32.8767123287671, Blast_Score=124, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321412, Length=273, Percent_Identity=40.2930402930403, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_RHILO (Q98CN6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105799.1
- ProteinModelPortal:   Q98CN6
- SMR:   Q98CN6
- GeneID:   1228460
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5073
- NMPDR:   fig|266835.1.peg.3903
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29130; Mature: 28999

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMA
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHEECCCCCCCCC
DGPAIQAAGLRALKGGQTLVKTLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDAL
CCCCCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
ASGIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTDDKRLPKVLQNTSGFVYYVSM
HCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEE
TGITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNA
CCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCEEEHHHHHHH
VANVLGPKGEKTADPAEAVATLVSGLAQGVRSARLAAAE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMA
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHEECCCCCCCCC
DGPAIQAAGLRALKGGQTLVKTLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDAL
CCCCCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
ASGIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTDDKRLPKVLQNTSGFVYYVSM
HCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEE
TGITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNA
CCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCEEEHHHHHHH
VANVLGPKGEKTADPAEAVATLVSGLAQGVRSARLAAAE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968