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The map label for this gene is caiD [H]

Identifier: 13474207

GI number: 13474207

Start: 3994906

End: 3995733

Strand: Direct

Name: caiD [H]

Synonym: mlr5044

Alternate gene names: 13474207

Gene position: 3994906-3995733 (Clockwise)

Preceding gene: 13474206

Following gene: 13474208

Centisome position: 56.78

GC content: 65.82

Gene sequence:

>828_bases
GTGCCTGGATCGCCGACGCGCTGCTGGTGCTCGGAGAAAACAAGAATGTCTGACGTCATTTCGACCCGCCGCGAAGGCTC
GATTCTCGAGGTCACGCTCGACCGGCCCAAGGCCAACGCCATCGACCTGAAGACCTCGCGGCTGATGGGGCAAACCTTCA
AGGCGTTCCGCGACGATCCGGAACTGCGCGTCGCCATCGTCAAGACATCAGGCGACAAGTTCTTCTGCGCCGGCTGGGAC
CTGAAGGCCGCCGCCGGCGGCGACGCGGTCGACGGCGACTACGGCGTCGGCGGCTTCGCCGGCCTGCAGGAATTGCGCGA
CTTGAACAAGCCGGTCATCGCTTGCGTAAACGGCATGGCGGTGGGCGGCGGCTTCGAACTGGCGCTGTCCTGCGATCTCA
TCTACGCCTCCGACCACTCCTCCTTCGCGCTGCCCGAAATTCGCGCCGGCACGCTGGCCGACGCGGCGACGATCAAGCTG
CCGAAGCGCATTCCCTACCATGTCGCCATGGACCTCCTGCTCACCGGCCGCTGGATGGATGTCGCGGAGGCACATCGCTG
GGGCCTGGTCAACGAGGTGCTGCCCAAGGAAAAGCTCGAGGACCGCGTCTGGGAGATCGCCCGCCTGCTCGCCAGCGGCC
CGCCGCTGGTCTTTGCCGCGATCAAGGAGACCGCGCGGGTGGCCGAAGCGCTGACCTTCCAGGACGCCATGAACAGGGTA
ACGCGCCGCCAGTTGGCGACGGTCGATGCGCTCTACGGCTCCGAGGACAATATGGAAGGGTTCCGCGCCTTCGCCGAAAA
GCGCGATCCGGTGTGGAAGGGAAAGTGA

Upstream 100 bases:

>100_bases
ATCGATGCTATCGCGGGCATTGCCGACTTCGTGCAGAAGAATGCCGGCGAGATCGAGGAACTCGACATCAATCCGCTGAT
CGTCTGCGTCGAAGGCAAGG

Downstream 100 bases:

>100_bases
GCCGCCTTACCCTCCCCTTGATGGGGAGGGTCGGACCGAAGGTCCGGGGTGGGGTGAAGCGCCGCCACCCCCACCCGCCG
CTCCGCGGCGACCTCCCCCC

Product: carnitinyl-CoA dehydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPGSPTRCWCSEKTRMSDVISTRREGSILEVTLDRPKANAIDLKTSRLMGQTFKAFRDDPELRVAIVKTSGDKFFCAGWD
LKAAAGGDAVDGDYGVGGFAGLQELRDLNKPVIACVNGMAVGGGFELALSCDLIYASDHSSFALPEIRAGTLADAATIKL
PKRIPYHVAMDLLLTGRWMDVAEAHRWGLVNEVLPKEKLEDRVWEIARLLASGPPLVFAAIKETARVAEALTFQDAMNRV
TRRQLATVDALYGSEDNMEGFRAFAEKRDPVWKGK

Sequences:

>Translated_275_residues
MPGSPTRCWCSEKTRMSDVISTRREGSILEVTLDRPKANAIDLKTSRLMGQTFKAFRDDPELRVAIVKTSGDKFFCAGWD
LKAAAGGDAVDGDYGVGGFAGLQELRDLNKPVIACVNGMAVGGGFELALSCDLIYASDHSSFALPEIRAGTLADAATIKL
PKRIPYHVAMDLLLTGRWMDVAEAHRWGLVNEVLPKEKLEDRVWEIARLLASGPPLVFAAIKETARVAEALTFQDAMNRV
TRRQLATVDALYGSEDNMEGFRAFAEKRDPVWKGK
>Mature_274_residues
PGSPTRCWCSEKTRMSDVISTRREGSILEVTLDRPKANAIDLKTSRLMGQTFKAFRDDPELRVAIVKTSGDKFFCAGWDL
KAAAGGDAVDGDYGVGGFAGLQELRDLNKPVIACVNGMAVGGGFELALSCDLIYASDHSSFALPEIRAGTLADAATIKLP
KRIPYHVAMDLLLTGRWMDVAEAHRWGLVNEVLPKEKLEDRVWEIARLLASGPPLVFAAIKETARVAEALTFQDAMNRVT
RRQLATVDALYGSEDNMEGFRAFAEKRDPVWKGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=277, Percent_Identity=33.5740072202166, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=29.811320754717, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=260, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=29.7619047619048, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=29.3577981651376, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=29.3577981651376, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=29.3577981651376, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=29.5081967213115, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=26.8041237113402, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=26.8041237113402, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20127408, Length=205, Percent_Identity=26.3414634146341, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=52.1072796934866, Blast_Score=267, Evalue=5e-73,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=33.7121212121212, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=31.5175097276265, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788597, Length=268, Percent_Identity=28.7313432835821, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=30.9701492537313, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=29.0816326530612, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=34.0080971659919, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=269, Percent_Identity=30.8550185873606, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=27.5303643724696, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=28.4552845528455, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=29.0196078431373, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=27.3469387755102, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=197, Percent_Identity=29.4416243654822, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=289, Percent_Identity=25.9515570934256, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=34.156378600823, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=34.156378600823, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653139, Length=276, Percent_Identity=31.1594202898551, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357171, Length=201, Percent_Identity=32.8358208955224, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=29.1304347826087, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=23.4615384615385, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=31.1224489795918, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=205, Percent_Identity=28.2926829268293, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571729, Length=205, Percent_Identity=28.2926829268293, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921000, Length=214, Percent_Identity=25.2336448598131, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 30149; Mature: 30018

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGSPTRCWCSEKTRMSDVISTRREGSILEVTLDRPKANAIDLKTSRLMGQTFKAFRDDP
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEECHHHHHHHHHHHHHCCCC
ELRVAIVKTSGDKFFCAGWDLKAAAGGDAVDGDYGVGGFAGLQELRDLNKPVIACVNGMA
CEEEEEEEECCCEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHEEHHCCEE
VGGGFELALSCDLIYASDHSSFALPEIRAGTLADAATIKLPKRIPYHVAMDLLLTGRWMD
ECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHCCCCHH
VAEAHRWGLVNEVLPKEKLEDRVWEIARLLASGPPLVFAAIKETARVAEALTFQDAMNRV
HHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
TRRQLATVDALYGSEDNMEGFRAFAEKRDPVWKGK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PGSPTRCWCSEKTRMSDVISTRREGSILEVTLDRPKANAIDLKTSRLMGQTFKAFRDDP
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEECHHHHHHHHHHHHHCCCC
ELRVAIVKTSGDKFFCAGWDLKAAAGGDAVDGDYGVGGFAGLQELRDLNKPVIACVNGMA
CEEEEEEEECCCEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHEEHHCCEE
VGGGFELALSCDLIYASDHSSFALPEIRAGTLADAATIKLPKRIPYHVAMDLLLTGRWMD
ECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHCCCCHH
VAEAHRWGLVNEVLPKEKLEDRVWEIARLLASGPPLVFAAIKETARVAEALTFQDAMNRV
HHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
TRRQLATVDALYGSEDNMEGFRAFAEKRDPVWKGK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA