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The map label for this gene is emrB [H]

Identifier: 13474035

GI number: 13474035

Start: 3832161

End: 3833756

Strand: Direct

Name: emrB [H]

Synonym: mlr4819

Alternate gene names: 13474035

Gene position: 3832161-3833756 (Clockwise)

Preceding gene: 13474034

Following gene: 13474040

Centisome position: 54.46

GC content: 63.47

Gene sequence:

>1596_bases
ATGGCAACCGCAACGATCACCGCAGGAGCGCCGCCGGCAAGACCGGCGGTCCCTGACACCATCTCCACACGCCGCGTCAT
CGCCTTCCTGGCGATGGTGTTCGGCATGTTCATGGCGATCCTGGACATCCAGATCGTCTCGGCCTCGCTGGCCGAGATCC
AGGCGGGCCTCAGCGCCAGCTCCGATGAAATTCCGTGGGTGCAGACGGCCTATCTGATCGCCGAGGTGGTGATGATCCCG
CTCTCGGGCTTCCTTAGCCGGATGCTGTCGACGCGCGTGCTGTTCACCATCGCCGCCGCCGGCTTCACCGCCGCCAGCGC
GCTTGCCGCGACCGCCACCAACATCGATCAGATGATCGTCTACCGCGCCGTGCAGGGCTTCATCGGCGGCGGCATGATCC
CGAGCGTCTTTGCCGCGGCCTTCACCATCTTCCCGCCCTCCAAGCGCGCCGTCGTCTCGCCCATGATCGGCCTGGTGGCG
ACGCTGGCGCCGACCATCGGCCCGACCGTCGGCGGCTATATCAGTCACGCCTTTTCATGGCATTGGCTGTTCCTGGTCAA
CGTCGTGCCCGGCATCCTGGTGGCGACCGCCGCCTGGTCGCTGATCGACTTCGACAAGCCCAATCTCAAGCTGTTCAACA
AGTTCGACTGGTGGGGCCTCGCCGGCATGGCGGCTTTCCTCGGCTGCATGGAATATGTGCTGGAAGAAGGCCCCAACAAT
GACTGGCTGCAGGACCAGGGTGTGTTCATCTGCGCCATCGTCATGACCATCGGCGCGGTGATCTTCTTCTGGCGCGTCTT
CACCGCCGAGGAGCCGATCGTCGACCTCAGGGCCTTCAGCAACGTCAACTTCGCCTTCGGCTCGCTGTTTTCCTTCGTCA
TCGGCATTGGCCTCTACGGGCTGACCTATCTCTATCCGGTCTTCCTCGGCCGTATCCGCGGCTATGATTCGATGATGATC
GGCGAGGCGCTGTTCGTCAGCGGCCTGGCGATGTTCGTCACCGCGCCGATTTCGGGCTTCCTGTCGAGCAAGATCGATTT
GCGGCTGATGATGATGATCGGCTTCTTCGGCTTCGCCACCGGCACCTGGTGGATGACGCATCTGACCGCCGACTGGGATT
TCTACGAGCTGCTCATCCCGCAGATCCTGCGCGGCTGTTCGATGATGCTGTGCATGGTGCCGATCAACAACATCGCGCTC
GGCACCTTGCCGCCCGACCGGCTGAAGAACGCATCCGGCCTGTTCAACCTCACCCGCAACCTCGGCGGTGCCGTCGGCCT
TGCCCTCATCAACACCGTGCTGATCAACCGCAACGCCTTCCATTATGCGAGGCTCGGCGAGCATGTGCAGTGGGGCAGTG
CGGCCGCGCAGACCAAGCTGCAGAACATGACGCTCAACTTCGAGCAGACATCAGGCCTCGACGCGGCCGGTGCGGCGATC
TCCAAGCTGTCGGGCATGGTCCATCAGCAGGCGGCGCTGCTGTCCTTCATGGACGTGTTCTACATGCTGACGGTGCTGTT
CGCGACGCTCGGCCTGTTCGCGATGCTGATCAACAAGCCGGCCGCGCCTGGTGGCGGCGGGGGTGGCGGGCATTAA

Upstream 100 bases:

>100_bases
CACCGCGCCTGCCGCGACGACCAACTAAGCGCTTTCGGAGGGTGGCTCGGCGGCTGATGAAGCGCCGGGCCGCCCGGCCT
GCCTCAAGGAGGCCCATGCA

Downstream 100 bases:

>100_bases
CGGAAGAACCATACGCGACAGCGGTTTCGGGAAGCTCCTCCAGTCCACGCCCGAAAAGCTCTGACCGCGAAAAAAACTCC
GGGCCGCAAGACCCGGAGTT

Product: multidrug resistance protein B (drug efflux transporter)

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MATATITAGAPPARPAVPDTISTRRVIAFLAMVFGMFMAILDIQIVSASLAEIQAGLSASSDEIPWVQTAYLIAEVVMIP
LSGFLSRMLSTRVLFTIAAAGFTAASALAATATNIDQMIVYRAVQGFIGGGMIPSVFAAAFTIFPPSKRAVVSPMIGLVA
TLAPTIGPTVGGYISHAFSWHWLFLVNVVPGILVATAAWSLIDFDKPNLKLFNKFDWWGLAGMAAFLGCMEYVLEEGPNN
DWLQDQGVFICAIVMTIGAVIFFWRVFTAEEPIVDLRAFSNVNFAFGSLFSFVIGIGLYGLTYLYPVFLGRIRGYDSMMI
GEALFVSGLAMFVTAPISGFLSSKIDLRLMMMIGFFGFATGTWWMTHLTADWDFYELLIPQILRGCSMMLCMVPINNIAL
GTLPPDRLKNASGLFNLTRNLGGAVGLALINTVLINRNAFHYARLGEHVQWGSAAAQTKLQNMTLNFEQTSGLDAAGAAI
SKLSGMVHQQAALLSFMDVFYMLTVLFATLGLFAMLINKPAAPGGGGGGGH

Sequences:

>Translated_531_residues
MATATITAGAPPARPAVPDTISTRRVIAFLAMVFGMFMAILDIQIVSASLAEIQAGLSASSDEIPWVQTAYLIAEVVMIP
LSGFLSRMLSTRVLFTIAAAGFTAASALAATATNIDQMIVYRAVQGFIGGGMIPSVFAAAFTIFPPSKRAVVSPMIGLVA
TLAPTIGPTVGGYISHAFSWHWLFLVNVVPGILVATAAWSLIDFDKPNLKLFNKFDWWGLAGMAAFLGCMEYVLEEGPNN
DWLQDQGVFICAIVMTIGAVIFFWRVFTAEEPIVDLRAFSNVNFAFGSLFSFVIGIGLYGLTYLYPVFLGRIRGYDSMMI
GEALFVSGLAMFVTAPISGFLSSKIDLRLMMMIGFFGFATGTWWMTHLTADWDFYELLIPQILRGCSMMLCMVPINNIAL
GTLPPDRLKNASGLFNLTRNLGGAVGLALINTVLINRNAFHYARLGEHVQWGSAAAQTKLQNMTLNFEQTSGLDAAGAAI
SKLSGMVHQQAALLSFMDVFYMLTVLFATLGLFAMLINKPAAPGGGGGGGH
>Mature_530_residues
ATATITAGAPPARPAVPDTISTRRVIAFLAMVFGMFMAILDIQIVSASLAEIQAGLSASSDEIPWVQTAYLIAEVVMIPL
SGFLSRMLSTRVLFTIAAAGFTAASALAATATNIDQMIVYRAVQGFIGGGMIPSVFAAAFTIFPPSKRAVVSPMIGLVAT
LAPTIGPTVGGYISHAFSWHWLFLVNVVPGILVATAAWSLIDFDKPNLKLFNKFDWWGLAGMAAFLGCMEYVLEEGPNND
WLQDQGVFICAIVMTIGAVIFFWRVFTAEEPIVDLRAFSNVNFAFGSLFSFVIGIGLYGLTYLYPVFLGRIRGYDSMMIG
EALFVSGLAMFVTAPISGFLSSKIDLRLMMMIGFFGFATGTWWMTHLTADWDFYELLIPQILRGCSMMLCMVPINNIALG
TLPPDRLKNASGLFNLTRNLGGAVGLALINTVLINRNAFHYARLGEHVQWGSAAAQTKLQNMTLNFEQTSGLDAAGAAIS
KLSGMVHQQAALLSFMDVFYMLTVLFATLGLFAMLINKPAAPGGGGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=27.4949083503055, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788710, Length=500, Percent_Identity=27.8, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87081983, Length=421, Percent_Identity=27.3159144893112, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790195, Length=415, Percent_Identity=23.855421686747, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788392, Length=420, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57048; Mature: 56917

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATATITAGAPPARPAVPDTISTRRVIAFLAMVFGMFMAILDIQIVSASLAEIQAGLSAS
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDEIPWVQTAYLIAEVVMIPLSGFLSRMLSTRVLFTIAAAGFTAASALAATATNIDQMIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAVQGFIGGGMIPSVFAAAFTIFPPSKRAVVSPMIGLVATLAPTIGPTVGGYISHAFSW
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HWLFLVNVVPGILVATAAWSLIDFDKPNLKLFNKFDWWGLAGMAAFLGCMEYVLEEGPNN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
DWLQDQGVFICAIVMTIGAVIFFWRVFTAEEPIVDLRAFSNVNFAFGSLFSFVIGIGLYG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
LTYLYPVFLGRIRGYDSMMIGEALFVSGLAMFVTAPISGFLSSKIDLRLMMMIGFFGFAT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
GTWWMTHLTADWDFYELLIPQILRGCSMMLCMVPINNIALGTLPPDRLKNASGLFNLTRN
HHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCCHHHHHHHHH
LGGAVGLALINTVLINRNAFHYARLGEHVQWGSAAAQTKLQNMTLNFEQTSGLDAAGAAI
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCEEEEHHCCCCCHHHHHH
SKLSGMVHQQAALLSFMDVFYMLTVLFATLGLFAMLINKPAAPGGGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATATITAGAPPARPAVPDTISTRRVIAFLAMVFGMFMAILDIQIVSASLAEIQAGLSAS
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDEIPWVQTAYLIAEVVMIPLSGFLSRMLSTRVLFTIAAAGFTAASALAATATNIDQMIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAVQGFIGGGMIPSVFAAAFTIFPPSKRAVVSPMIGLVATLAPTIGPTVGGYISHAFSW
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HWLFLVNVVPGILVATAAWSLIDFDKPNLKLFNKFDWWGLAGMAAFLGCMEYVLEEGPNN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
DWLQDQGVFICAIVMTIGAVIFFWRVFTAEEPIVDLRAFSNVNFAFGSLFSFVIGIGLYG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
LTYLYPVFLGRIRGYDSMMIGEALFVSGLAMFVTAPISGFLSSKIDLRLMMMIGFFGFAT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
GTWWMTHLTADWDFYELLIPQILRGCSMMLCMVPINNIALGTLPPDRLKNASGLFNLTRN
HHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCCHHHHHHHHH
LGGAVGLALINTVLINRNAFHYARLGEHVQWGSAAAQTKLQNMTLNFEQTSGLDAAGAAI
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCEEEEHHCCCCCHHHHHH
SKLSGMVHQQAALLSFMDVFYMLTVLFATLGLFAMLINKPAAPGGGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]