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The map label for this gene is nylA [H]

Identifier: 13474040

GI number: 13474040

Start: 3836762

End: 3838258

Strand: Direct

Name: nylA [H]

Synonym: mlr4825

Alternate gene names: 13474040

Gene position: 3836762-3838258 (Clockwise)

Preceding gene: 13474035

Following gene: 13474041

Centisome position: 54.53

GC content: 68.34

Gene sequence:

>1497_bases
ATGTCGGTCGAACGGATCCTGTGGGAAGAAGACGCGACCGGCCTCGCCGGCCTGGTGCGCAAGGGCGAGCTGTCGGCGAT
CGAGTTGACCGAAGCGGCGATCGCGCGCGCCGAGGCCACAAGGCCTGAAATCAATGCCACGGCCGAGCCGCTCTACGAAG
CCGCGCGGGCGCGGGCGAAAACCATGGACCGCTCGCTGCCGCTGGCCGGCGTGCCCTTTGCCATCAAGGACCTCGGCATC
GCCGTCAAGGGTGTGCCCTCGCATGGCGGCAGCCGCATTCCGGCCTCGGTGCCCGACGTCAATTCGGTGATGACCGACCG
CTATCTGGCCGCCGGGCTGATCCCGATCGTCACCTCGACATCACCGGAACACGGTCTTCGGCTGATGACCGAATCCAGGG
CCTTCGGCATCACCCGCAATCCCTGGAACACCGGCCACACCAGCGGCGGCTCGTCGGGTGGCGCGGCGGCCCTGGTCGCT
GCCGGCGTGGTGCCGGTGGCGCATGCCTCCGATGGCGGCGGCTCGATCCGCGTGCCTTCCGCCTGCACGGGCCTGGTCGG
CCTCAAGACCTCGCGCGGCCGCATCCCGCTGACGCCGCTGGTCAGCGAGAGCTGGTACGGCATGGTGGTCGACCACGCCG
TCACCCGCTCGGTGCGCGATAGCGCGCTGCTGCTCGACCTCACCCACGGTCCCGACTCCCTGTCGCCCTATGCGGCATTG
CCGCCGAAGGGCACATTCGCCGCCGCCGCCGCGCGCGATCCCGGCAAGCTCAGCCTGGCCGTCTACCGCAAGTCGCCGCT
TGGCCTGCCGATCTCGGCCGAGACGATGAAAGCGCTCGACACGGCCGTGGTGCTTGCGCGCGAGGGCGGTCACACCGTCA
TGGAGATCGACCTCCCTTTCATAGGCCGCGATTTCCTGGCCGATTTCTGCAGGACGGTTGCTTCCGCCGTCGCCGGCACG
CTGCGGGCCGAAGCGCTGCGCGTCGGCCGTTCCGTTACCGGCGACGTCGAGCGCGCCACGCGGGTGCTCGGCCGGTTGGG
CGAAATGGTCTCGGCCGGCGAGATCTATGCCGGCCTGCAGCGGCTGCATGCCGCCTCGCGCCGGATGATCGAGGAAACCG
CGCAGTATGACGCCGTGCTGATGCCTGTTATCGCGCATCCGCCGCTCGCCTGCGGCGCCTTGGATCCGAAAGGCGCCGAC
GAGCTGATCGAAAACCTGCTCGACAAGCTTCATCTGACGCCTTTGCTGAAGCTGAAACCCTTGTTCGGCCAGCTGATGGA
CAAGAGCCTTTTGTTCACGCACTGGCCGGCAATCCACAATGTCAGCGGCCAGCCCTCGATCGCGCTGCCTGTCCATGTCA
CCGAGGCCGGCCTGCCGCTTGGCATCCAAGCCGCCGGGCGCCCGGGCGACGAGGAGACGCTTTTGTCGTTTGCCGCGCAG
ATGGAGAAGCTTTCGGGCTGGCTGAAACGGCGGGCGCCGCTGATGGTGCCGTCATGA

Upstream 100 bases:

>100_bases
CGGAAATATTCCTTGCACCTAAAGCTCCGGTGTCCAGGCGCAGGCTTCGATTGCCAAGGGATTAGCCGCTGGGTAGCTTG
GCGGGAAGGGGGAAGCGCCG

Downstream 100 bases:

>100_bases
TGACGAGGCCGTGAACGCGGCCAATATGACAGCAATGCCATTTGCCCCCCGCGCCAATTCCCGCTAAGACGCCGCCGTCT
TAATCTGATGCATGTCGCCC

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSVERILWEEDATGLAGLVRKGELSAIELTEAAIARAEATRPEINATAEPLYEAARARAKTMDRSLPLAGVPFAIKDLGI
AVKGVPSHGGSRIPASVPDVNSVMTDRYLAAGLIPIVTSTSPEHGLRLMTESRAFGITRNPWNTGHTSGGSSGGAAALVA
AGVVPVAHASDGGGSIRVPSACTGLVGLKTSRGRIPLTPLVSESWYGMVVDHAVTRSVRDSALLLDLTHGPDSLSPYAAL
PPKGTFAAAAARDPGKLSLAVYRKSPLGLPISAETMKALDTAVVLAREGGHTVMEIDLPFIGRDFLADFCRTVASAVAGT
LRAEALRVGRSVTGDVERATRVLGRLGEMVSAGEIYAGLQRLHAASRRMIEETAQYDAVLMPVIAHPPLACGALDPKGAD
ELIENLLDKLHLTPLLKLKPLFGQLMDKSLLFTHWPAIHNVSGQPSIALPVHVTEAGLPLGIQAAGRPGDEETLLSFAAQ
MEKLSGWLKRRAPLMVPS

Sequences:

>Translated_498_residues
MSVERILWEEDATGLAGLVRKGELSAIELTEAAIARAEATRPEINATAEPLYEAARARAKTMDRSLPLAGVPFAIKDLGI
AVKGVPSHGGSRIPASVPDVNSVMTDRYLAAGLIPIVTSTSPEHGLRLMTESRAFGITRNPWNTGHTSGGSSGGAAALVA
AGVVPVAHASDGGGSIRVPSACTGLVGLKTSRGRIPLTPLVSESWYGMVVDHAVTRSVRDSALLLDLTHGPDSLSPYAAL
PPKGTFAAAAARDPGKLSLAVYRKSPLGLPISAETMKALDTAVVLAREGGHTVMEIDLPFIGRDFLADFCRTVASAVAGT
LRAEALRVGRSVTGDVERATRVLGRLGEMVSAGEIYAGLQRLHAASRRMIEETAQYDAVLMPVIAHPPLACGALDPKGAD
ELIENLLDKLHLTPLLKLKPLFGQLMDKSLLFTHWPAIHNVSGQPSIALPVHVTEAGLPLGIQAAGRPGDEETLLSFAAQ
MEKLSGWLKRRAPLMVPS
>Mature_497_residues
SVERILWEEDATGLAGLVRKGELSAIELTEAAIARAEATRPEINATAEPLYEAARARAKTMDRSLPLAGVPFAIKDLGIA
VKGVPSHGGSRIPASVPDVNSVMTDRYLAAGLIPIVTSTSPEHGLRLMTESRAFGITRNPWNTGHTSGGSSGGAAALVAA
GVVPVAHASDGGGSIRVPSACTGLVGLKTSRGRIPLTPLVSESWYGMVVDHAVTRSVRDSALLLDLTHGPDSLSPYAALP
PKGTFAAAAARDPGKLSLAVYRKSPLGLPISAETMKALDTAVVLAREGGHTVMEIDLPFIGRDFLADFCRTVASAVAGTL
RAEALRVGRSVTGDVERATRVLGRLGEMVSAGEIYAGLQRLHAASRRMIEETAQYDAVLMPVIAHPPLACGALDPKGADE
LIENLLDKLHLTPLLKLKPLFGQLMDKSLLFTHWPAIHNVSGQPSIALPVHVTEAGLPLGIQAAGRPGDEETLLSFAAQM
EKLSGWLKRRAPLMVPS

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI222831590, Length=524, Percent_Identity=24.4274809160305, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=523, Percent_Identity=24.8565965583174, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=310, Percent_Identity=27.4193548387097, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=194, Percent_Identity=30.9278350515464, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=26.1146496815287, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=314, Percent_Identity=26.1146496815287, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32565337, Length=454, Percent_Identity=24.8898678414097, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=517, Percent_Identity=22.2437137330754, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=182, Percent_Identity=34.0659340659341, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644968, Length=530, Percent_Identity=24.7169811320755, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648435, Length=532, Percent_Identity=23.4962406015038, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648437, Length=532, Percent_Identity=23.4962406015038, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI45550774, Length=533, Percent_Identity=23.827392120075, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=509, Percent_Identity=24.5579567779961, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=24.853228962818, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24652985, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922090, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24652981, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24652983, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=26.8686868686869, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648441, Length=465, Percent_Identity=22.7956989247312, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648439, Length=465, Percent_Identity=22.7956989247312, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52318; Mature: 52187

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVERILWEEDATGLAGLVRKGELSAIELTEAAIARAEATRPEINATAEPLYEAARARAK
CCCHHHEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
TMDRSLPLAGVPFAIKDLGIAVKGVPSHGGSRIPASVPDVNSVMTDRYLAAGLIPIVTST
HHHCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHEEECC
SPEHGLRLMTESRAFGITRNPWNTGHTSGGSSGGAAALVAAGVVPVAHASDGGGSIRVPS
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCEEECCH
ACTGLVGLKTSRGRIPLTPLVSESWYGMVVDHAVTRSVRDSALLLDLTHGPDSLSPYAAL
HHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
PPKGTFAAAAARDPGKLSLAVYRKSPLGLPISAETMKALDTAVVLAREGGHTVMEIDLPF
CCCCCEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEEECCC
IGRDFLADFCRTVASAVAGTLRAEALRVGRSVTGDVERATRVLGRLGEMVSAGEIYAGLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLHAASRRMIEETAQYDAVLMPVIAHPPLACGALDPKGADELIENLLDKLHLTPLLKLKP
HHHHHHHHHHHHHHHHCCEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
LFGQLMDKSLLFTHWPAIHNVSGQPSIALPVHVTEAGLPLGIQAAGRPGDEETLLSFAAQ
HHHHHHCCHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
MEKLSGWLKRRAPLMVPS
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVERILWEEDATGLAGLVRKGELSAIELTEAAIARAEATRPEINATAEPLYEAARARAK
CCHHHEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
TMDRSLPLAGVPFAIKDLGIAVKGVPSHGGSRIPASVPDVNSVMTDRYLAAGLIPIVTST
HHHCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHEEECC
SPEHGLRLMTESRAFGITRNPWNTGHTSGGSSGGAAALVAAGVVPVAHASDGGGSIRVPS
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCEEECCH
ACTGLVGLKTSRGRIPLTPLVSESWYGMVVDHAVTRSVRDSALLLDLTHGPDSLSPYAAL
HHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
PPKGTFAAAAARDPGKLSLAVYRKSPLGLPISAETMKALDTAVVLAREGGHTVMEIDLPF
CCCCCEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEEECCC
IGRDFLADFCRTVASAVAGTLRAEALRVGRSVTGDVERATRVLGRLGEMVSAGEIYAGLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLHAASRRMIEETAQYDAVLMPVIAHPPLACGALDPKGADELIENLLDKLHLTPLLKLKP
HHHHHHHHHHHHHHHHCCEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
LFGQLMDKSLLFTHWPAIHNVSGQPSIALPVHVTEAGLPLGIQAAGRPGDEETLLSFAAQ
HHHHHHCCHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
MEKLSGWLKRRAPLMVPS
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]