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The map label for this gene is emrK [H]

Identifier: 13474034

GI number: 13474034

Start: 3830901

End: 3832088

Strand: Direct

Name: emrK [H]

Synonym: mlr4818

Alternate gene names: 13474034

Gene position: 3830901-3832088 (Clockwise)

Preceding gene: 13474032

Following gene: 13474035

Centisome position: 54.45

GC content: 66.25

Gene sequence:

>1188_bases
ATGTCCTCGAACGCGCCCAACACAGCCGAAGTCCGCCCCTTCCCTAACGCCAAGGTCGCTCCGGCGACGGAAGCACCGGA
AATTCCCGTCCTGCCCGTTGCACCGCCGCCGGCGGCTGAAGCTCCGGCCAAGAAGAAGCGCTCGGTGCGCTCCTTCCTGC
TGCCGATCATCGGCCTTGCCCTGTTGAGCGGCGGCGCCTGGTATGGCTACGACTATTGGACCACGGGCCGTTTCATGATC
TCGACAGACGATGCCTATGTCCAGGCCGACATGGCGTTCGTTTCGCCCAAGATCTCCGGCTATGTCGATCAGGTCAAGGT
CAGCGAGAACCAGCAGGTCAAGGCCGGCGACCCGCTGCTGACGATCGACGATGGCGACTACAAGATTGCCGTTGCCCAGG
CCGAGGCGCAGATCGCCACATTGTCCAAGACGCTCGACCGCATCGACGCGCAGACCAAGGCCGCGCAGGCTTCCCTGCAG
CAGGCCAAGGCGCAGAAGGTCGCCGACCAGGCCGCTGCCGACAATGCCGCCCGTGCCCAGCAGCGCGCCGCGCAGTTGGT
CAAGACGCATGTCGGCACGCAGGCGCAGCTGGACGATGCCCAGACCGCGCTCGACCAGGCCAATGCCGCTCTTGTCGGAG
CAGATGCCCAGATCGCCGCCGCACAGGCCAATATCGGCGTGCTCGAGGCGCAGCGCGCCGAGTCGGCAAGCACGCTCGCT
TCGCTGCAGCTCGGCCGCGACAAGGCCGCGCGCGACCTTTCCTTCACCGTGCTCAAGGCGCCTTATGACGGCATTGTCGG
CAACCGTTCCGTCGAGCAGGGCGACCTCGTCAGCCCCGGCCAGAAGCTCGCCGTCGTCGTGCCGATGGACAAGCTCTACG
TCGTCGCCAACTTCAAGGAGACGCAGCTCGCCCGGCTGGTTCCCGGCGAAAAGGTCAACATCTCGGTCGACGCCATCGAC
GGCCATCCCATCCAGGGCACCGTCTCGTCGCTGGCTCCGGCTTCAGGCGCGGTCTTCTCGCTGTTGCCGCCGGAAAACGC
CACCGGCAACTTCACCAAGGTCGTGCAGCGCGTCCCGGTCCGCATCGACGTCCCGGCCGATGCCCTGAAGACCGGCAAGC
TGCGTGCGGGCTTGAGCGTTGTCGTCAACGTTGACAGCCGCACCGCGCCTGCCGCGACGACCAACTAA

Upstream 100 bases:

>100_bases
CCAACCGGTTCGATTGCCTCTTGATATGGTCCTCTTTCCACGCTATGTCAACGGCCTGATCGAACCGAACGGTTCAGTAT
TATTACTTTAGAGAGATGTC

Downstream 100 bases:

>100_bases
GCGCTTTCGGAGGGTGGCTCGGCGGCTGATGAAGCGCCGGGCCGCCCGGCCTGCCTCAAGGAGGCCCATGCAATGGCAAC
CGCAACGATCACCGCAGGAG

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSSNAPNTAEVRPFPNAKVAPATEAPEIPVLPVAPPPAAEAPAKKKRSVRSFLLPIIGLALLSGGAWYGYDYWTTGRFMI
STDDAYVQADMAFVSPKISGYVDQVKVSENQQVKAGDPLLTIDDGDYKIAVAQAEAQIATLSKTLDRIDAQTKAAQASLQ
QAKAQKVADQAAADNAARAQQRAAQLVKTHVGTQAQLDDAQTALDQANAALVGADAQIAAAQANIGVLEAQRAESASTLA
SLQLGRDKAARDLSFTVLKAPYDGIVGNRSVEQGDLVSPGQKLAVVVPMDKLYVVANFKETQLARLVPGEKVNISVDAID
GHPIQGTVSSLAPASGAVFSLLPPENATGNFTKVVQRVPVRIDVPADALKTGKLRAGLSVVVNVDSRTAPAATTN

Sequences:

>Translated_395_residues
MSSNAPNTAEVRPFPNAKVAPATEAPEIPVLPVAPPPAAEAPAKKKRSVRSFLLPIIGLALLSGGAWYGYDYWTTGRFMI
STDDAYVQADMAFVSPKISGYVDQVKVSENQQVKAGDPLLTIDDGDYKIAVAQAEAQIATLSKTLDRIDAQTKAAQASLQ
QAKAQKVADQAAADNAARAQQRAAQLVKTHVGTQAQLDDAQTALDQANAALVGADAQIAAAQANIGVLEAQRAESASTLA
SLQLGRDKAARDLSFTVLKAPYDGIVGNRSVEQGDLVSPGQKLAVVVPMDKLYVVANFKETQLARLVPGEKVNISVDAID
GHPIQGTVSSLAPASGAVFSLLPPENATGNFTKVVQRVPVRIDVPADALKTGKLRAGLSVVVNVDSRTAPAATTN
>Mature_394_residues
SSNAPNTAEVRPFPNAKVAPATEAPEIPVLPVAPPPAAEAPAKKKRSVRSFLLPIIGLALLSGGAWYGYDYWTTGRFMIS
TDDAYVQADMAFVSPKISGYVDQVKVSENQQVKAGDPLLTIDDGDYKIAVAQAEAQIATLSKTLDRIDAQTKAAQASLQQ
AKAQKVADQAAADNAARAQQRAAQLVKTHVGTQAQLDDAQTALDQANAALVGADAQIAAAQANIGVLEAQRAESASTLAS
LQLGRDKAARDLSFTVLKAPYDGIVGNRSVEQGDLVSPGQKLAVVVPMDKLYVVANFKETQLARLVPGEKVNISVDAIDG
HPIQGTVSSLAPASGAVFSLLPPENATGNFTKVVQRVPVRIDVPADALKTGKLRAGLSVVVNVDSRTAPAATTN

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=357, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789041, Length=367, Percent_Identity=31.8801089918256, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790519, Length=314, Percent_Identity=30.8917197452229, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789637, Length=325, Percent_Identity=27.3846153846154, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081951, Length=333, Percent_Identity=26.7267267267267, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790024, Length=244, Percent_Identity=25.4098360655738, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790012, Length=248, Percent_Identity=26.2096774193548, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787013, Length=280, Percent_Identity=27.8571428571429, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789900, Length=290, Percent_Identity=26.551724137931, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41260; Mature: 41129

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNAPNTAEVRPFPNAKVAPATEAPEIPVLPVAPPPAAEAPAKKKRSVRSFLLPIIGLA
CCCCCCCCCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLSGGAWYGYDYWTTGRFMISTDDAYVQADMAFVSPKISGYVDQVKVSENQQVKAGDPLL
HHCCCCEECEEEEECCEEEEECCCCEEEEHHHHCCCCHHCCCEEEEECCCCEEECCCCEE
TIDDGDYKIAVAQAEAQIATLSKTLDRIDAQTKAAQASLQQAKAQKVADQAAADNAARAQ
EEECCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAAQLVKTHVGTQAQLDDAQTALDQANAALVGADAQIAAAQANIGVLEAQRAESASTLA
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEHHHHHHHHHHH
SLQLGRDKAARDLSFTVLKAPYDGIVGNRSVEQGDLVSPGQKLAVVVPMDKLYVVANFKE
HHHHCCCHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCH
TQLARLVPGEKVNISVDAIDGHPIQGTVSSLAPASGAVFSLLPPENATGNFTKVVQRVPV
HHHHHHCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCE
RIDVPADALKTGKLRAGLSVVVNVDSRTAPAATTN
EEECCHHHHHCCCEECCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SSNAPNTAEVRPFPNAKVAPATEAPEIPVLPVAPPPAAEAPAKKKRSVRSFLLPIIGLA
CCCCCCCCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLSGGAWYGYDYWTTGRFMISTDDAYVQADMAFVSPKISGYVDQVKVSENQQVKAGDPLL
HHCCCCEECEEEEECCEEEEECCCCEEEEHHHHCCCCHHCCCEEEEECCCCEEECCCCEE
TIDDGDYKIAVAQAEAQIATLSKTLDRIDAQTKAAQASLQQAKAQKVADQAAADNAARAQ
EEECCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAAQLVKTHVGTQAQLDDAQTALDQANAALVGADAQIAAAQANIGVLEAQRAESASTLA
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEHHHHHHHHHHH
SLQLGRDKAARDLSFTVLKAPYDGIVGNRSVEQGDLVSPGQKLAVVVPMDKLYVVANFKE
HHHHCCCHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCH
TQLARLVPGEKVNISVDAIDGHPIQGTVSSLAPASGAVFSLLPPENATGNFTKVVQRVPV
HHHHHHCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCE
RIDVPADALKTGKLRAGLSVVVNVDSRTAPAATTN
EEECCHHHHHCCCEECCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]