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The map label for this gene is purA

Identifier: 13473317

GI number: 13473317

Start: 3108809

End: 3110107

Strand: Reverse

Name: purA

Synonym: mll3873

Alternate gene names: 13473317

Gene position: 3110107-3108809 (Counterclockwise)

Preceding gene: 13473319

Following gene: 13473316

Centisome position: 44.2

GC content: 64.82

Gene sequence:

>1299_bases
ATGGCCAATGTGGTGGTCGTCGGCTCGCAATGGGGCGACGAAGGCAAGGGCAAGATCGTCGACTGGCTGTCGGAGCGCGC
CGATGTGGTGGTGCGTTTCCAGGGTGGCCACAATGCCGGCCACACGCTGGTCGTCGACGGCAAGGTCTACAAGCTGTCGC
TGTTGCCGTCCGGCGTCGTGCGGCAGGGCAAGCTGTCCATCATCGGCAATGGCGTCGTCTTCGACCCGCATGCTTTCGTG
GCCGAAGTGGCGAAGCTCAAGGCGCAGGGCGTCGAAGTGACGCCGGATCGCCTGAAAATAGCCGAAAACACAGCGCTTAT
CCTGTCGCTGCACCGGGAGCTGGACGGATTCCGCGAGGACGCCGCGTCAAATTCCGGGACGAAGATTGGCACGACCCGCC
GGGGCATTGGTCCGGCCTATGAGGACAAGGTGGGTCGGCGCGCGGTGCGGGTGATGGATCTGGCGGATTTGGAAACGCTT
CCCCTGAAGGTCGACAGGCTGCTGACGCACCACAATGCGCTGCGCCGCGGGCTTGGCCATGGCGAAGTCACACATGACGC
GATCATGGCTGAATTGACCTCGGTCGCCGACGAGATCCTGCCCTATATGGACCGTGTCTGGAAAATCCTCGACGACAAGC
GCCGCGCCGGCGAGCGCATCCTGTTCGAAGGCGCGCAAGGCACGCTGCTCGACATCGACCACGGCACCTATCCCTTCGTC
ACCTCGTCCAACACGGTGGCCGGCCAGGCCGCCGCCGGCTCGGGCGTCGGCCCGGGCGCCATCGGCTACGTGCTCGGCAT
TACCAAGGCCTACACGACGCGCGTCGGCGAAGGCCCGTTCCCGACCGAACAGAAGAACGAGATCGGCGAGTTCCTCGGCA
CGCGCGGTCATGAATTCGGCGTCGTCACCGGGCGTAAGCGCCGCTGCGGCTGGTTCGACGCGGTGCTGGTGCGCCAGGCC
GTCGCCGTCAACGGCATCAAGGGCATCGCGCTGACCAAGCTCGACGTGCTCGATGGGCTGGACGAGATCAAGGTCTGCAC
CGGCTATCGCCTCGACGGCGAGATGATCGACTATTTGCCGGCCAGCCAGGGCGCGCAGGCCCGTGTCGAACCGGTCTACG
AGACGCTGGAAGGGTGGAAAGGCACCACTGCCGGCGCGCGCAGCTGGAACGACCTTCCGGCCCAGGCCGTCAAATATGTC
CGATACATAGAGGAGCTGATCGGCGCGCCAGTGGCACTCCTCTCCACCAGCCCGGAACGGGACGATACGATACTTGTGAC
CGATCCGTTTCAAGACTAG

Upstream 100 bases:

>100_bases
CGGCAAAGAAAGGTCGCACTCGCGAGGTCTTGACGCTATAGAGGCCTGTCGTTTCGAACCATATCCAAGCCGCTCGGCGG
CGTCTCAAGGGAAAAGAAAC

Downstream 100 bases:

>100_bases
TTTCTGAAGCCTTTCCGGGCATCACGGCTGATTTGCGGGGGCCGGCGCGGAAGCAAAAATACAGGTCGACTGAAGCATGG
CAGATTTTGTTGCTGTTCTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLVVDGKVYKLSLLPSGVVRQGKLSIIGNGVVFDPHAFV
AEVAKLKAQGVEVTPDRLKIAENTALILSLHRELDGFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAVRVMDLADLETL
PLKVDRLLTHHNALRRGLGHGEVTHDAIMAELTSVADEILPYMDRVWKILDDKRRAGERILFEGAQGTLLDIDHGTYPFV
TSSNTVAGQAAAGSGVGPGAIGYVLGITKAYTTRVGEGPFPTEQKNEIGEFLGTRGHEFGVVTGRKRRCGWFDAVLVRQA
VAVNGIKGIALTKLDVLDGLDEIKVCTGYRLDGEMIDYLPASQGAQARVEPVYETLEGWKGTTAGARSWNDLPAQAVKYV
RYIEELIGAPVALLSTSPERDDTILVTDPFQD

Sequences:

>Translated_432_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLVVDGKVYKLSLLPSGVVRQGKLSIIGNGVVFDPHAFV
AEVAKLKAQGVEVTPDRLKIAENTALILSLHRELDGFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAVRVMDLADLETL
PLKVDRLLTHHNALRRGLGHGEVTHDAIMAELTSVADEILPYMDRVWKILDDKRRAGERILFEGAQGTLLDIDHGTYPFV
TSSNTVAGQAAAGSGVGPGAIGYVLGITKAYTTRVGEGPFPTEQKNEIGEFLGTRGHEFGVVTGRKRRCGWFDAVLVRQA
VAVNGIKGIALTKLDVLDGLDEIKVCTGYRLDGEMIDYLPASQGAQARVEPVYETLEGWKGTTAGARSWNDLPAQAVKYV
RYIEELIGAPVALLSTSPERDDTILVTDPFQD
>Mature_431_residues
ANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLVVDGKVYKLSLLPSGVVRQGKLSIIGNGVVFDPHAFVA
EVAKLKAQGVEVTPDRLKIAENTALILSLHRELDGFREDAASNSGTKIGTTRRGIGPAYEDKVGRRAVRVMDLADLETLP
LKVDRLLTHHNALRRGLGHGEVTHDAIMAELTSVADEILPYMDRVWKILDDKRRAGERILFEGAQGTLLDIDHGTYPFVT
SSNTVAGQAAAGSGVGPGAIGYVLGITKAYTTRVGEGPFPTEQKNEIGEFLGTRGHEFGVVTGRKRRCGWFDAVLVRQAV
AVNGIKGIALTKLDVLDGLDEIKVCTGYRLDGEMIDYLPASQGAQARVEPVYETLEGWKGTTAGARSWNDLPAQAVKYVR
YIEELIGAPVALLSTSPERDDTILVTDPFQD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=46.3356973995272, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=44.2352941176471, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=44.9238578680203, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=52.093023255814, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=43.8228438228438, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=43.8228438228438, Blast_Score=351, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=42.3611111111111, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.8679245283019, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_RHILO (Q98F97)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104884.1
- ProteinModelPortal:   Q98F97
- SMR:   Q98F97
- GeneID:   1227545
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3873
- NMPDR:   fig|266835.1.peg.2988
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46672; Mature: 46540

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 132-132 BINDING 146-146 BINDING 226-226 BINDING 241-241 BINDING 305-305 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLVVDGKVYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCEEEEEEECCCCCC
RQGKLSIIGNGVVFDPHAFVAEVAKLKAQGVEVTPDRLKIAENTALILSLHRELDGFRED
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCEECHHHEEECCCCEEEEEHHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAVRVMDLADLETLPLKVDRLLTHHNALRRGLGH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
GEVTHDAIMAELTSVADEILPYMDRVWKILDDKRRAGERILFEGAQGTLLDIDHGTYPFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGVGPGAIGYVLGITKAYTTRVGEGPFPTEQKNEIGEFLGTRGHEFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEE
VVTGRKRRCGWFDAVLVRQAVAVNGIKGIALTKLDVLDGLDEIKVCTGYRLDGEMIDYLP
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHCCCEEECCHHHHCCC
ASQGAQARVEPVYETLEGWKGTTAGARSWNDLPAQAVKYVRYIEELIGAPVALLSTSPER
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFQD
CCEEEEECCCCC
>Mature Secondary Structure 
ANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLVVDGKVYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCEEEEEEECCCCCC
RQGKLSIIGNGVVFDPHAFVAEVAKLKAQGVEVTPDRLKIAENTALILSLHRELDGFRED
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCEECHHHEEECCCCEEEEEHHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAVRVMDLADLETLPLKVDRLLTHHNALRRGLGH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
GEVTHDAIMAELTSVADEILPYMDRVWKILDDKRRAGERILFEGAQGTLLDIDHGTYPFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGVGPGAIGYVLGITKAYTTRVGEGPFPTEQKNEIGEFLGTRGHEFG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEE
VVTGRKRRCGWFDAVLVRQAVAVNGIKGIALTKLDVLDGLDEIKVCTGYRLDGEMIDYLP
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHCCCEEECCHHHHCCC
ASQGAQARVEPVYETLEGWKGTTAGARSWNDLPAQAVKYVRYIEELIGAPVALLSTSPER
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFQD
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968