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The map label for this gene is lutR [H]

Identifier: 13472918

GI number: 13472918

Start: 2705947

End: 2706666

Strand: Direct

Name: lutR [H]

Synonym: mlr3366

Alternate gene names: 13472918

Gene position: 2705947-2706666 (Clockwise)

Preceding gene: 13472917

Following gene: 13472919

Centisome position: 38.46

GC content: 63.61

Gene sequence:

>720_bases
ATGAAAGAAAAGAACCTTCTCGCGGAACTCGCAGCCTATCTGTTCTCCCACTCGGACAAGGAGAGCGGCCGCACCCCATC
CGAACGTGAGCTGGCGGAGCATTTCGCCGTCAGCCGCGGCCAGATCCGCGAGGCGCTGGCCATCCTCGAAGCCATGCGCA
TCGTCGAGCGGCGCGCCAAATCCGGCATCTACATCGACACCAAGCAGGCGAGCGTCGAGGCGCTGGCGCTGTTCGCGCGC
GCCGGCCTGCCGCTCGATCCCTTGCAGATCTACGAGACGGTCGAGTTGCGCAAGATCCACGAGATCAAGGCCGCCGAACT
TGCCTGCTCGCGCGCCACCGAGGAGAATTTCGAGCGGTTGCGCGAGATCCTCAAGGCTTCCGAGGAACGCATCGCTGCCG
GCGAAGGTCTGGCCAAGGAGGACCGCGAGTTCCACCTGGAGATTGTGCGCGCCACCAAGAACAGCGTTTTCCACAACATC
TGCAGCGTCTACTATCTGATGGGCGAACAGCGCCTGCCGATCTATTTCAACGATCCCGAACGCAGCGTGCGTTCGCATGC
CGAGCATGTGCAGATCTACGAGGCGCTGCTGCGCCGTGACGGCAATCTCGCCCAGGCGCTGATGAGCGCCCACCTGCAAG
GGGCGGAGAGCTATTGGAAGGGCCTGATCGAGGGCGGGAATGCGGCTGCGCCCGCGAACTCGGCGCTCGAACAGGCCTGA

Upstream 100 bases:

>100_bases
TCGAAGCACAGGACGGTATGATAGCGATCCCGGAAAAGCCCGGATTGGGATTCACCGTTTCTGAGCGATTCCTGGAGGCG
CACGCGCAACGCAATTGACG

Downstream 100 bases:

>100_bases
GCGTGGCCCGGACCGTGCCCAAGATCCTGTCGACCCATCCGCTGCATCCGCGCGCCTCGGCCAAGCTGGCCGGCGCCGGC
CAGCTCGTCATCGCATCGGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKEKNLLAELAAYLFSHSDKESGRTPSERELAEHFAVSRGQIREALAILEAMRIVERRAKSGIYIDTKQASVEALALFAR
AGLPLDPLQIYETVELRKIHEIKAAELACSRATEENFERLREILKASEERIAAGEGLAKEDREFHLEIVRATKNSVFHNI
CSVYYLMGEQRLPIYFNDPERSVRSHAEHVQIYEALLRRDGNLAQALMSAHLQGAESYWKGLIEGGNAAAPANSALEQA

Sequences:

>Translated_239_residues
MKEKNLLAELAAYLFSHSDKESGRTPSERELAEHFAVSRGQIREALAILEAMRIVERRAKSGIYIDTKQASVEALALFAR
AGLPLDPLQIYETVELRKIHEIKAAELACSRATEENFERLREILKASEERIAAGEGLAKEDREFHLEIVRATKNSVFHNI
CSVYYLMGEQRLPIYFNDPERSVRSHAEHVQIYEALLRRDGNLAQALMSAHLQGAESYWKGLIEGGNAAAPANSALEQA
>Mature_239_residues
MKEKNLLAELAAYLFSHSDKESGRTPSERELAEHFAVSRGQIREALAILEAMRIVERRAKSGIYIDTKQASVEALALFAR
AGLPLDPLQIYETVELRKIHEIKAAELACSRATEENFERLREILKASEERIAAGEGLAKEDREFHLEIVRATKNSVFHNI
CSVYYLMGEQRLPIYFNDPERSVRSHAEHVQIYEALLRRDGNLAQALMSAHLQGAESYWKGLIEGGNAAAPANSALEQA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=201, Percent_Identity=29.8507462686567, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082208, Length=207, Percent_Identity=29.4685990338164, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26832; Mature: 26832

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKNLLAELAAYLFSHSDKESGRTPSERELAEHFAVSRGQIREALAILEAMRIVERRAK
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIYIDTKQASVEALALFARAGLPLDPLQIYETVELRKIHEIKAAELACSRATEENFERL
CCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REILKASEERIAAGEGLAKEDREFHLEIVRATKNSVFHNICSVYYLMGEQRLPIYFNDPE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
RSVRSHAEHVQIYEALLRRDGNLAQALMSAHLQGAESYWKGLIEGGNAAAPANSALEQA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MKEKNLLAELAAYLFSHSDKESGRTPSERELAEHFAVSRGQIREALAILEAMRIVERRAK
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIYIDTKQASVEALALFARAGLPLDPLQIYETVELRKIHEIKAAELACSRATEENFERL
CCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REILKASEERIAAGEGLAKEDREFHLEIVRATKNSVFHNICSVYYLMGEQRLPIYFNDPE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
RSVRSHAEHVQIYEALLRRDGNLAQALMSAHLQGAESYWKGLIEGGNAAAPANSALEQA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]