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The map label for this gene is ptsN [H]

Identifier: 13472786

GI number: 13472786

Start: 2555838

End: 2556302

Strand: Reverse

Name: ptsN [H]

Synonym: mll3194

Alternate gene names: 13472786

Gene position: 2556302-2555838 (Counterclockwise)

Preceding gene: 13472787

Following gene: 13472783

Centisome position: 36.33

GC content: 64.73

Gene sequence:

>465_bases
ATGGATCTGAGCGATCTTATCAGCGTTCCGGCGATTTTGCCGGCGTTGAAGGCGAACTCCAAAAAGCAGCTTCTGCAATT
GCTTTCCGAGCGGGCGGCGGCGATTTCGGGCATTCCGGAGAGGGAAGTGTTCGACACCATCCTGCAGCGTGAGCGGCTGG
GCTCGACGGGCGTCGGCAATGGCATCGCCATTCCGCATGGCAAGCTCGCCGGCGTGAAGCGCATCGCCGGTGTTTTCGCC
CGGCTGGAAACACCGGTCGATTTCGAGTCGCTGGACGACCAGCCTGTCGATCTGGTGTTTCTGCTCCTGGCGCCGGAAGG
GGCGGGCGCCGATCATCTGAAGGCCTTGTCGCGCATCGCACGCGTGCTGCGCGACGCTGACACGGTGGCCAAGATCAGGG
GGACGCGCGACGCCACGGCGATCCACGCGCTGCTGTCGGACACGCAGGCCTCGCACGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCTGGATCGACCCGTCCACGACCAAGGTCGCACAGGGATAAAGCCAGCCGCACGAGGGGTGGGGACTGGCGACGGCAGG
CGAACAAGGGATTTTCAAGC

Downstream 100 bases:

>100_bases
AGTTGGATTTTGAGAAAGACTGCCAAGGGCCGCCGCAAGCGGCCCTTTTCGTTTCAGCCAACCGGTCGCTTTAGTGAATG
GCGACGGTGGTCAGGTCGTT

Product: phosphotransferase system enzyme II

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDLSDLISVPAILPALKANSKKQLLQLLSERAAAISGIPEREVFDTILQRERLGSTGVGNGIAIPHGKLAGVKRIAGVFA
RLETPVDFESLDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTVAKIRGTRDATAIHALLSDTQASHAA

Sequences:

>Translated_154_residues
MDLSDLISVPAILPALKANSKKQLLQLLSERAAAISGIPEREVFDTILQRERLGSTGVGNGIAIPHGKLAGVKRIAGVFA
RLETPVDFESLDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTVAKIRGTRDATAIHALLSDTQASHAA
>Mature_154_residues
MDLSDLISVPAILPALKANSKKQLLQLLSERAAAISGIPEREVFDTILQRERLGSTGVGNGIAIPHGKLAGVKRIAGVFA
RLETPVDFESLDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDADTVAKIRGTRDATAIHALLSDTQASHAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=152, Percent_Identity=40.1315789473684, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1786951, Length=136, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788726, Length=146, Percent_Identity=30.1369863013699, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI2367327, Length=111, Percent_Identity=30.6306306306306, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16302; Mature: 16302

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSDLISVPAILPALKANSKKQLLQLLSERAAAISGIPEREVFDTILQRERLGSTGVGN
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLAGVKRIAGVFARLETPVDFESLDDQPVDLVFLLLAPEGAGADHLKALSRIA
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RVLRDADTVAKIRGTRDATAIHALLSDTQASHAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLSDLISVPAILPALKANSKKQLLQLLSERAAAISGIPEREVFDTILQRERLGSTGVGN
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLAGVKRIAGVFARLETPVDFESLDDQPVDLVFLLLAPEGAGADHLKALSRIA
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RVLRDADTVAKIRGTRDATAIHALLSDTQASHAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]