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The map label for this gene is rihA [H]

Identifier: 13472783

GI number: 13472783

Start: 2553824

End: 2554765

Strand: Reverse

Name: rihA [H]

Synonym: mll3190

Alternate gene names: 13472783

Gene position: 2554765-2553824 (Counterclockwise)

Preceding gene: 13472786

Following gene: 13472777

Centisome position: 36.31

GC content: 62.1

Gene sequence:

>942_bases
ATGCCCCAACCACGCAAGATCATCATCGACACCGATCCCGGCCAGGATGATGCCGTCGCCATTCTCTTGGCGCTCGGCAG
CAGCGAACTGGAGATCGTCGGCATCACCGCCGTTGCCGGCAACGTGCCGCTGAAGCTTACCGAAAAGAATGCCCGCAAGA
TTTGCGAACTGGCCGGCCGGCCAGAAATGAAAGTCTATGCCGGTGCCATCCGCCCCCTGGCGCGCACACTGGTCACCGCA
GAGGAAGTGCATGGCAAGACCGGCCTCAACGGCCCACAGCTGCCCGAACCGACGATGAAACTGCAGGAGCAGTATGCCGT
CGACTTCATCGTCGAGACGCTGATGAAGGAGGAGAGCGGTACCATTACGCTCTGCCCGCTCGGGCCGCTGACCAACATCG
CACTGGCGCTGATCCGCGAACCACGGATCGCGCCGCGCATCAAGGAAATCGTGCTGATGGGCGGCGGTTTCTTCGAGGGC
GGCAACGTCACGCCCGCGGCCGAATTCAACATTTATGTCGATCCGCAGGCCGCCGATCTGGTGTTCAAGTCCGGCATCCC
GATCGTGATGATGCCACTCGACGTCACCCACAAGGCGTTGACCACCACCAAGCGCACGCAGGCCTTCCGCGCGCTCGGCA
CCAAGGTCGGTATCGCGACCGCCGAGATGCTGGAATTCTTCGAGCGCTACGACGAGGGCAAATACGGCACCGATGGCGGC
CCGCTGCACGACCCCTGCGTCATCGCCTATCTCCTGAAGCCGGAGCTGTTCAAGGGCCGCAACTGCAATGTCAGCGTTGA
GACCGCCTCGGAACTGACCATGGGCATGACCGTGATCGACTGGTGGGGTGTCACCAAGCGGGAAAAGAACGCCATGGTGA
TGCGCGAAATCGACCATGACGGCTTCTTCGCGTTGCTGGTGGAGAGGCTGGGGCGGCTTTAG

Upstream 100 bases:

>100_bases
GGCGCTTGAAACGACGCGCGGATGCAAGGAGAAGCGCTGCGGTCGTCCTTCAGGCGTGCGATCGATCCGGTGAGTTCACG
GCCCCAAACAAGGAATTGAA

Downstream 100 bases:

>100_bases
GGGGTCTTTTTACCTTCTCCCCTTGCGGGAGAAGGTGGCCTCGCGAAGCGAGGTCGGATGAGGGGTGATCCAGGGAACGC
CAACGCCTCACTCCGTCACG

Product: nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPQPRKIIIDTDPGQDDAVAILLALGSSELEIVGITAVAGNVPLKLTEKNARKICELAGRPEMKVYAGAIRPLARTLVTA
EEVHGKTGLNGPQLPEPTMKLQEQYAVDFIVETLMKEESGTITLCPLGPLTNIALALIREPRIAPRIKEIVLMGGGFFEG
GNVTPAAEFNIYVDPQAADLVFKSGIPIVMMPLDVTHKALTTTKRTQAFRALGTKVGIATAEMLEFFERYDEGKYGTDGG
PLHDPCVIAYLLKPELFKGRNCNVSVETASELTMGMTVIDWWGVTKREKNAMVMREIDHDGFFALLVERLGRL

Sequences:

>Translated_313_residues
MPQPRKIIIDTDPGQDDAVAILLALGSSELEIVGITAVAGNVPLKLTEKNARKICELAGRPEMKVYAGAIRPLARTLVTA
EEVHGKTGLNGPQLPEPTMKLQEQYAVDFIVETLMKEESGTITLCPLGPLTNIALALIREPRIAPRIKEIVLMGGGFFEG
GNVTPAAEFNIYVDPQAADLVFKSGIPIVMMPLDVTHKALTTTKRTQAFRALGTKVGIATAEMLEFFERYDEGKYGTDGG
PLHDPCVIAYLLKPELFKGRNCNVSVETASELTMGMTVIDWWGVTKREKNAMVMREIDHDGFFALLVERLGRL
>Mature_312_residues
PQPRKIIIDTDPGQDDAVAILLALGSSELEIVGITAVAGNVPLKLTEKNARKICELAGRPEMKVYAGAIRPLARTLVTAE
EVHGKTGLNGPQLPEPTMKLQEQYAVDFIVETLMKEESGTITLCPLGPLTNIALALIREPRIAPRIKEIVLMGGGFFEGG
NVTPAAEFNIYVDPQAADLVFKSGIPIVMMPLDVTHKALTTTKRTQAFRALGTKVGIATAEMLEFFERYDEGKYGTDGGP
LHDPCVIAYLLKPELFKGRNCNVSVETASELTMGMTVIDWWGVTKREKNAMVMREIDHDGFFALLVERLGRL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=305, Percent_Identity=48.8524590163934, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=41.1764705882353, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=40.1960784313725, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32565474, Length=199, Percent_Identity=34.1708542713568, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17565698, Length=291, Percent_Identity=27.4914089347079, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=33.0739299610895, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24641837, Length=338, Percent_Identity=27.810650887574, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24583915, Length=205, Percent_Identity=30.2439024390244, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24641839, Length=206, Percent_Identity=28.1553398058252, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34205; Mature: 34074

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPRKIIIDTDPGQDDAVAILLALGSSELEIVGITAVAGNVPLKLTEKNARKICELAGR
CCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCEEEECHHHHHHHHHCCC
PEMKVYAGAIRPLARTLVTAEEVHGKTGLNGPQLPEPTMKLQEQYAVDFIVETLMKEESG
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TITLCPLGPLTNIALALIREPRIAPRIKEIVLMGGGFFEGGNVTPAAEFNIYVDPQAADL
CEEEEECCCHHHHHHHHHCCCCCCHHHEEEEEECCCEECCCCCCCCEEEEEEECCCHHHH
VFKSGIPIVMMPLDVTHKALTTTKRTQAFRALGTKVGIATAEMLEFFERYDEGKYGTDGG
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLHDPCVIAYLLKPELFKGRNCNVSVETASELTMGMTVIDWWGVTKREKNAMVMREIDHD
CCCCHHHEEEECCHHHHCCCCCCEEEECHHHHHHCEEEEEECCCCCCCCCCEEEEECCCC
GFFALLVERLGRL
CHHHHHHHHHCCC
>Mature Secondary Structure 
PQPRKIIIDTDPGQDDAVAILLALGSSELEIVGITAVAGNVPLKLTEKNARKICELAGR
CCCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCEEEECHHHHHHHHHCCC
PEMKVYAGAIRPLARTLVTAEEVHGKTGLNGPQLPEPTMKLQEQYAVDFIVETLMKEESG
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TITLCPLGPLTNIALALIREPRIAPRIKEIVLMGGGFFEGGNVTPAAEFNIYVDPQAADL
CEEEEECCCHHHHHHHHHCCCCCCHHHEEEEEECCCEECCCCCCCCEEEEEEECCCHHHH
VFKSGIPIVMMPLDVTHKALTTTKRTQAFRALGTKVGIATAEMLEFFERYDEGKYGTDGG
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLHDPCVIAYLLKPELFKGRNCNVSVETASELTMGMTVIDWWGVTKREKNAMVMREIDHD
CCCCHHHEEEECCHHHHCCCCCCEEEECHHHHHHCEEEEEECCCCCCCCCCEEEEECCCC
GFFALLVERLGRL
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA