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The map label for this gene is punA [H]

Identifier: 13472757

GI number: 13472757

Start: 2530108

End: 2530917

Strand: Direct

Name: punA [H]

Synonym: mlr3159

Alternate gene names: 13472757

Gene position: 2530108-2530917 (Clockwise)

Preceding gene: 13472756

Following gene: 13472758

Centisome position: 35.96

GC content: 67.04

Gene sequence:

>810_bases
ATGACCGAAAAGGCGGTGGACCATCTGATCGAAAGGCTGGACGGCCTGGCGCCGTCGACGGCGCTGGTGCTGGGGTCGGG
CCTCGGCGTCCTTGTCGACCGGATCGAGCACCCGATCCGCGTCCCCTATGCCGACCTGCCCGGGTTCCCCCGGAGCGGCG
TCAGCGGCCATGCCGGCGAAGTGGTGGCGGGCCTGTTTGGCGGAAAGCCGGTGCTGATGCTGTCCGGCCGCGCCCATTAT
TACGAGCATGGCAACGCGGCGGCGATGCGCCCGGTGCTGGAAGTGCTCGCCGGCATCGGCATCACCAAACTGATCCTCAC
CAACGCCGCCGGTTCGGTCGACCCGGACATGCCGCCGGGCTCGGTGATGATGCTCACCGACCATATCAATTTCTCCGGCA
GCAATCCGCTGATCGGCGAGCCCAGCGACCGCCGCTTCGTCGGCCTGACCGAAGCCTATGATGCCGGCATCCGCAAGGCG
ATCGAGCGCGCCGCCAAGGCGACCGGTACCGCGCTGCACAAGGGCGTCTATATGTGGTTTTCCGGCCCGTGTTTCGAAAC
GCCGGCCGAAATCCGCATGGCGCGCGTCATGGGCGCCAACGCCGTCGGCATGTCGACCGTGCCGGAAGTCATTCTTGCCC
GCTTCCTTGGCCTGCGCGTCGCCGCCTGTTCGGTCATCACCAATCTGGCGGCCGGCATGACCGGAGCCGAGCTTTCGCAT
CAGGAGACCAAGGACATGGCGCCAGTCGGCGGGTCGCGGCTGGCAACGGTCCTGCACAGAGTGTTCCGCGACGGACTGCT
GGAGAGCTGA

Upstream 100 bases:

>100_bases
GGGCGTGGCCGAGACCGTAACCATGGGCGACATGCTGCCCTATGGTTTCCGCGGCGACATCCTGAAATGAGGAATTGGCT
CAAATGAAGAGTGGGCTGCA

Downstream 100 bases:

>100_bases
TGCTTCCGCAGGAAATCATCCGCCACAAGCGAGACGGCCACAGGCTCTCGGCCGGTGAAATCGCTGCCTTCATCGGCGGC
GTTACCTCGGGCGCCGTCAC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTEKAVDHLIERLDGLAPSTALVLGSGLGVLVDRIEHPIRVPYADLPGFPRSGVSGHAGEVVAGLFGGKPVLMLSGRAHY
YEHGNAAAMRPVLEVLAGIGITKLILTNAAGSVDPDMPPGSVMMLTDHINFSGSNPLIGEPSDRRFVGLTEAYDAGIRKA
IERAAKATGTALHKGVYMWFSGPCFETPAEIRMARVMGANAVGMSTVPEVILARFLGLRVAACSVITNLAAGMTGAELSH
QETKDMAPVGGSRLATVLHRVFRDGLLES

Sequences:

>Translated_269_residues
MTEKAVDHLIERLDGLAPSTALVLGSGLGVLVDRIEHPIRVPYADLPGFPRSGVSGHAGEVVAGLFGGKPVLMLSGRAHY
YEHGNAAAMRPVLEVLAGIGITKLILTNAAGSVDPDMPPGSVMMLTDHINFSGSNPLIGEPSDRRFVGLTEAYDAGIRKA
IERAAKATGTALHKGVYMWFSGPCFETPAEIRMARVMGANAVGMSTVPEVILARFLGLRVAACSVITNLAAGMTGAELSH
QETKDMAPVGGSRLATVLHRVFRDGLLES
>Mature_268_residues
TEKAVDHLIERLDGLAPSTALVLGSGLGVLVDRIEHPIRVPYADLPGFPRSGVSGHAGEVVAGLFGGKPVLMLSGRAHYY
EHGNAAAMRPVLEVLAGIGITKLILTNAAGSVDPDMPPGSVMMLTDHINFSGSNPLIGEPSDRRFVGLTEAYDAGIRKAI
ERAAKATGTALHKGVYMWFSGPCFETPAEIRMARVMGANAVGMSTVPEVILARFLGLRVAACSVITNLAAGMTGAELSHQ
ETKDMAPVGGSRLATVLHRVFRDGLLES

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=219, Percent_Identity=42.9223744292237, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI1788746, Length=231, Percent_Identity=45.4545454545455, Blast_Score=201, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17541190, Length=241, Percent_Identity=37.344398340249, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323238, Length=224, Percent_Identity=41.5178571428571, Blast_Score=160, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656093, Length=274, Percent_Identity=38.3211678832117, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24656090, Length=274, Percent_Identity=38.3211678832117, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45552885, Length=269, Percent_Identity=38.2899628252788, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45552887, Length=274, Percent_Identity=38.3211678832117, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24762376, Length=251, Percent_Identity=34.2629482071713, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 28262; Mature: 28131

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKAVDHLIERLDGLAPSTALVLGSGLGVLVDRIEHPIRVPYADLPGFPRSGVSGHAGE
CCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
VVAGLFGGKPVLMLSGRAHYYEHGNAAAMRPVLEVLAGIGITKLILTNAAGSVDPDMPPG
HHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
SVMMLTDHINFSGSNPLIGEPSDRRFVGLTEAYDAGIRKAIERAAKATGTALHKGVYMWF
CEEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
SGPCFETPAEIRMARVMGANAVGMSTVPEVILARFLGLRVAACSVITNLAAGMTGAELSH
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCH
QETKDMAPVGGSRLATVLHRVFRDGLLES
HHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEKAVDHLIERLDGLAPSTALVLGSGLGVLVDRIEHPIRVPYADLPGFPRSGVSGHAGE
CHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
VVAGLFGGKPVLMLSGRAHYYEHGNAAAMRPVLEVLAGIGITKLILTNAAGSVDPDMPPG
HHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
SVMMLTDHINFSGSNPLIGEPSDRRFVGLTEAYDAGIRKAIERAAKATGTALHKGVYMWF
CEEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
SGPCFETPAEIRMARVMGANAVGMSTVPEVILARFLGLRVAACSVITNLAAGMTGAELSH
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCH
QETKDMAPVGGSRLATVLHRVFRDGLLES
HHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]