Click here to switch to the map view.

The map label for this gene is yafC [C]

Identifier: 13472623

GI number: 13472623

Start: 2392177

End: 2393076

Strand: Direct

Name: yafC [C]

Synonym: mlr2977

Alternate gene names: 13472623

Gene position: 2392177-2393076 (Clockwise)

Preceding gene: 13472622

Following gene: 13472624

Centisome position: 34.0

GC content: 66.11

Gene sequence:

>900_bases
ATGGACACACTGACCCGCATGCGCGCCTTCATCGACGTCGTCGAGGCCGAGGGCTTTTCGGCGGCGGCGCGCAAGATCGG
CCGCTCCAAGGCGCTGCTGTCGAAATATGTGCGCGAGCTGGAGGACGAACTCGGCGCGCTGCTGCTGAACCGCACGACGC
GCCAATTCTCGATGACCGAGGCCGGCCACACCTATTACCGGCGCGCCTCGGAGATCGTGCGCGAGGTCGACAGCCTGGCG
GACGCCGTGCGCGAATCCTCCGGCGACGTGCGCGGCAGGATCAAACTGTCGGCGCCGCGCACCTTCGCCGATGCGCCGAT
CGGGCAGTCGCTGATCGATTTCGCCAAGCAGCACCCCGACATCGTGCTCGACATCCAGCTCGACGACCGCTTCGTCGACC
TGGTCGAGGAAGGTTTCGATCTCGCGGTGCGCATCTCGCGCCTGGAGAACTCGTCGCTGATCGCGAGGCGGCTCGCGCCG
TTTTCGGTGAAACTCTGCGCCTCGCCCGAACTGATCGCCAAGCATGGCATGCCGACGCGGCCGCAGGATCTCGGCCACAT
GCCCTGTATCGTCGACACCAATGGCCGCGGGCTGAACAACTGGGCGTTCAAGGGCGACAACAACGATCAGCTGAGCGTCG
CGGTGGCAGGCCCGATCGAGGTCAACAGCCCGATGGCGGCGCGGGCCGCGGCCCTGTCGGGGCTCGGATTTTGCATCCTG
CCGGATTTCATCGCCTCGCCCGATCTCAAGAGTGGCCAGTTGGTGACAGCGCTCGACGACCGCATCCTGTCGGGCGGCGG
CATCTTCGCCGTCTACCCGCACCGCCGCTATCTGCCGGCCAAGGTCCGCGTTTTCGTCGACTTCCTGGTGCAATGGTTCA
GGACGCGTGAGGTCGCTTGA

Upstream 100 bases:

>100_bases
CGCACCGTTCGGCCTTTCGCTGCTTGGCCCTTCCGGCAGCGACATTGCCCTGATACGGCTCGGCCGAAAACTTCTCGACG
CAGCACAAAGGGCCTGAACC

Downstream 100 bases:

>100_bases
CCTGCCCAGGACTCTTGTGGCGTGCGTGTTGTAGGCGCAAAACCGCCGCGCACTTTTGGGTGACACGCTTTGGGGTTCGC
GCGTGTCACTGTCCCAAAAC

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGALLLNRTTRQFSMTEAGHTYYRRASEIVREVDSLA
DAVRESSGDVRGRIKLSAPRTFADAPIGQSLIDFAKQHPDIVLDIQLDDRFVDLVEEGFDLAVRISRLENSSLIARRLAP
FSVKLCASPELIAKHGMPTRPQDLGHMPCIVDTNGRGLNNWAFKGDNNDQLSVAVAGPIEVNSPMAARAAALSGLGFCIL
PDFIASPDLKSGQLVTALDDRILSGGGIFAVYPHRRYLPAKVRVFVDFLVQWFRTREVA

Sequences:

>Translated_299_residues
MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGALLLNRTTRQFSMTEAGHTYYRRASEIVREVDSLA
DAVRESSGDVRGRIKLSAPRTFADAPIGQSLIDFAKQHPDIVLDIQLDDRFVDLVEEGFDLAVRISRLENSSLIARRLAP
FSVKLCASPELIAKHGMPTRPQDLGHMPCIVDTNGRGLNNWAFKGDNNDQLSVAVAGPIEVNSPMAARAAALSGLGFCIL
PDFIASPDLKSGQLVTALDDRILSGGGIFAVYPHRRYLPAKVRVFVDFLVQWFRTREVA
>Mature_299_residues
MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGALLLNRTTRQFSMTEAGHTYYRRASEIVREVDSLA
DAVRESSGDVRGRIKLSAPRTFADAPIGQSLIDFAKQHPDIVLDIQLDDRFVDLVEEGFDLAVRISRLENSSLIARRLAP
FSVKLCASPELIAKHGMPTRPQDLGHMPCIVDTNGRGLNNWAFKGDNNDQLSVAVAGPIEVNSPMAARAAALSGLGFCIL
PDFIASPDLKSGQLVTALDDRILSGGGIFAVYPHRRYLPAKVRVFVDFLVQWFRTREVA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=32.0557491289199, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=33.6769759450172, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.0344827586207, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=31.1643835616438, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=32.3943661971831, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=27.3037542662116, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=27.4305555555556, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790011, Length=291, Percent_Identity=25.7731958762887, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789173, Length=271, Percent_Identity=26.1992619926199, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=21.8543046357616, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=134, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=24.4897959183673, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=119, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32985; Mature: 32985

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGALLLNRTTRQFSMTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AGHTYYRRASEIVREVDSLADAVRESSGDVRGRIKLSAPRTFADAPIGQSLIDFAKQHPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCC
IVLDIQLDDRFVDLVEEGFDLAVRISRLENSSLIARRLAPFSVKLCASPELIAKHGMPTR
EEEEEEECHHHHHHHHCCCCEEEEEEHHCCHHHHHHHCCCCEEEECCCCHHHHHCCCCCC
PQDLGHMPCIVDTNGRGLNNWAFKGDNNDQLSVAVAGPIEVNSPMAARAAALSGLGFCIL
CHHCCCCCEEEECCCCCCCCCEEECCCCCEEEEEEECCEECCCCHHHHHHHHHCCCHHCC
PDFIASPDLKSGQLVTALDDRILSGGGIFAVYPHRRYLPAKVRVFVDFLVQWFRTREVA
HHHHCCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTLTRMRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGALLLNRTTRQFSMTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AGHTYYRRASEIVREVDSLADAVRESSGDVRGRIKLSAPRTFADAPIGQSLIDFAKQHPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCC
IVLDIQLDDRFVDLVEEGFDLAVRISRLENSSLIARRLAPFSVKLCASPELIAKHGMPTR
EEEEEEECHHHHHHHHCCCCEEEEEEHHCCHHHHHHHCCCCEEEECCCCHHHHHCCCCCC
PQDLGHMPCIVDTNGRGLNNWAFKGDNNDQLSVAVAGPIEVNSPMAARAAALSGLGFCIL
CHHCCCCCEEEECCCCCCCCCEEECCCCCEEEEEEECCEECCCCHHHHHHHHHCCCHHCC
PDFIASPDLKSGQLVTALDDRILSGGGIFAVYPHRRYLPAKVRVFVDFLVQWFRTREVA
HHHHCCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]