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The map label for this gene is gatA [H]

Identifier: 13472622

GI number: 13472622

Start: 2390977

End: 2392173

Strand: Direct

Name: gatA [H]

Synonym: mlr2975

Alternate gene names: 13472622

Gene position: 2390977-2392173 (Clockwise)

Preceding gene: 13472619

Following gene: 13472623

Centisome position: 33.98

GC content: 67.5

Gene sequence:

>1197_bases
ATGACAGGCACGCACGGCCCCTACAACGCCTTCCTCGATCTTCGCCAGATGCCGGTGGCCCATGCCGAACTCGGCCCGCT
CGCCGGCCTGCGGCTGGCCGTCAAGGACATTTACGATGTCGCCGGCTACCGCACCGGATGCGGCAATTCCAGGAAATTCA
CCGAAAGCAATGCCGCCTCGCGCACGGCGCCGGCCGTCCAGACGATTCTCGATGCCGGCGCGCGCTTCGTCGGCAAGACG
CAGACCGACGAACTGGCCTTCGCGCTGTTCGGCCAGAACGCGCATTTTCCGTTTCCGGTGAACCCGGCGGCCCCCGACCG
CGTCACCGGCGGCTCGTCGTCCGGATCGGCATCAGCGGTGGCTGGCAGGCTGGCCGACATTGCCACCGGTTCCGACACCG
GCGGCTCGATCCGCGCGCCGGCGAGTTTCTGTGGCCTGATCGGGCTGCGCACCACGCATGGCCGTATCGCGCTCGATGGC
ACCATGAAGCTGGCGCCGAGCTTCGACACGTTCGGCTGGTTCGCCGACGACATCGAGACCTACGAGACCGTCGGCAAGCT
GCTGCTTGGCCGCGACCCGCATCAGCATTCCCTGGACCGCCCGATATCGCTCACCTGGCTGGACGAGATGGTTGCGCGCC
CGGCGCTTGGCGAATATGCCGCAATGAAGGCGCTGGCGGGTGCGGTTTTCGGTATGCCGGCGACCTCGACCACGCGCTTC
AGCTCTTCCCCTGATGAACTCTACTGGTGCTTTCGCCGGCTGCAGGCCAGGGAAGCCTGGGGCGTGCATGGCGAATGGAT
CACCAGCGGCGAGCGCGACCTTGGCCCCGGCGTCGAGGAGCGTTTCGGCTTTGGCCGTGCCGTCGACGACCGCACGGCGC
AGGCCGAAAACGTGCGCCGTCTGACCTTCCGCGGCGAACTCAGCGCCCTGCTCGGCCAGGACGGCTTCCTCGTCCTGCCG
ACGGTGCCCGGCCCGGCGCCCTATGTCGACTCGACGCCGGAGCAGTTCCAGGCCTATCGCGAGCGGGCCCTGCATCTTTT
GTGCCTCGCCGGCCTGTCAGGCTTCCCCCAAATCACCTTGCCCATCGGCTCGGTCGCCGGCGCACCGTTCGGCCTTTCGC
TGCTTGGCCCTTCCGGCAGCGACATTGCCCTGATACGGCTCGGCCGAAAACTTCTCGACGCAGCACAAAGGGCCTGA

Upstream 100 bases:

>100_bases
ATAGGGGCGAAGGACTTCACAATCAATAAAAATCGTATCGCAGGTTGTAAAATTTCCGGCATCGAACAATGTAGATGCAA
CCGTATCCGGATATCGAACA

Downstream 100 bases:

>100_bases
ACCATGGACACACTGACCCGCATGCGCGCCTTCATCGACGTCGTCGAGGCCGAGGGCTTTTCGGCGGCGGCGCGCAAGAT
CGGCCGCTCCAAGGCGCTGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTGTHGPYNAFLDLRQMPVAHAELGPLAGLRLAVKDIYDVAGYRTGCGNSRKFTESNAASRTAPAVQTILDAGARFVGKT
QTDELAFALFGQNAHFPFPVNPAAPDRVTGGSSSGSASAVAGRLADIATGSDTGGSIRAPASFCGLIGLRTTHGRIALDG
TMKLAPSFDTFGWFADDIETYETVGKLLLGRDPHQHSLDRPISLTWLDEMVARPALGEYAAMKALAGAVFGMPATSTTRF
SSSPDELYWCFRRLQAREAWGVHGEWITSGERDLGPGVEERFGFGRAVDDRTAQAENVRRLTFRGELSALLGQDGFLVLP
TVPGPAPYVDSTPEQFQAYRERALHLLCLAGLSGFPQITLPIGSVAGAPFGLSLLGPSGSDIALIRLGRKLLDAAQRA

Sequences:

>Translated_398_residues
MTGTHGPYNAFLDLRQMPVAHAELGPLAGLRLAVKDIYDVAGYRTGCGNSRKFTESNAASRTAPAVQTILDAGARFVGKT
QTDELAFALFGQNAHFPFPVNPAAPDRVTGGSSSGSASAVAGRLADIATGSDTGGSIRAPASFCGLIGLRTTHGRIALDG
TMKLAPSFDTFGWFADDIETYETVGKLLLGRDPHQHSLDRPISLTWLDEMVARPALGEYAAMKALAGAVFGMPATSTTRF
SSSPDELYWCFRRLQAREAWGVHGEWITSGERDLGPGVEERFGFGRAVDDRTAQAENVRRLTFRGELSALLGQDGFLVLP
TVPGPAPYVDSTPEQFQAYRERALHLLCLAGLSGFPQITLPIGSVAGAPFGLSLLGPSGSDIALIRLGRKLLDAAQRA
>Mature_397_residues
TGTHGPYNAFLDLRQMPVAHAELGPLAGLRLAVKDIYDVAGYRTGCGNSRKFTESNAASRTAPAVQTILDAGARFVGKTQ
TDELAFALFGQNAHFPFPVNPAAPDRVTGGSSSGSASAVAGRLADIATGSDTGGSIRAPASFCGLIGLRTTHGRIALDGT
MKLAPSFDTFGWFADDIETYETVGKLLLGRDPHQHSLDRPISLTWLDEMVARPALGEYAAMKALAGAVFGMPATSTTRFS
SSPDELYWCFRRLQAREAWGVHGEWITSGERDLGPGVEERFGFGRAVDDRTAQAENVRRLTFRGELSALLGQDGFLVLPT
VPGPAPYVDSTPEQFQAYRERALHLLCLAGLSGFPQITLPIGSVAGAPFGLSLLGPSGSDIALIRLGRKLLDAAQRA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=432, Percent_Identity=26.1574074074074, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=414, Percent_Identity=25.3623188405797, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=414, Percent_Identity=23.4299516908213, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=181, Percent_Identity=36.4640883977901, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=219, Percent_Identity=27.8538812785388, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=429, Percent_Identity=25.1748251748252, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 42294; Mature: 42163

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTHGPYNAFLDLRQMPVAHAELGPLAGLRLAVKDIYDVAGYRTGCGNSRKFTESNAAS
CCCCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
RTAPAVQTILDAGARFVGKTQTDELAFALFGQNAHFPFPVNPAAPDRVTGGSSSGSASAV
HHHHHHHHHHHHCHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGRLADIATGSDTGGSIRAPASFCGLIGLRTTHGRIALDGTMKLAPSFDTFGWFADDIET
HHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCEEEECCCEEECCCCCCCCCHHHHHHH
YETVGKLLLGRDPHQHSLDRPISLTWLDEMVARPALGEYAAMKALAGAVFGMPATSTTRF
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SSSPDELYWCFRRLQAREAWGVHGEWITSGERDLGPGVEERFGFGRAVDDRTAQAENVRR
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHEE
LTFRGELSALLGQDGFLVLPTVPGPAPYVDSTPEQFQAYRERALHLLCLAGLSGFPQITL
EEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PIGSVAGAPFGLSLLGPSGSDIALIRLGRKLLDAAQRA
ECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGTHGPYNAFLDLRQMPVAHAELGPLAGLRLAVKDIYDVAGYRTGCGNSRKFTESNAAS
CCCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
RTAPAVQTILDAGARFVGKTQTDELAFALFGQNAHFPFPVNPAAPDRVTGGSSSGSASAV
HHHHHHHHHHHHCHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGRLADIATGSDTGGSIRAPASFCGLIGLRTTHGRIALDGTMKLAPSFDTFGWFADDIET
HHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCEEEECCCEEECCCCCCCCCHHHHHHH
YETVGKLLLGRDPHQHSLDRPISLTWLDEMVARPALGEYAAMKALAGAVFGMPATSTTRF
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SSSPDELYWCFRRLQAREAWGVHGEWITSGERDLGPGVEERFGFGRAVDDRTAQAENVRR
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHEE
LTFRGELSALLGQDGFLVLPTVPGPAPYVDSTPEQFQAYRERALHLLCLAGLSGFPQITL
EEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PIGSVAGAPFGLSLLGPSGSDIALIRLGRKLLDAAQRA
ECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]