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The map label for this gene is potI [H]

Identifier: 13472554

GI number: 13472554

Start: 2326430

End: 2327230

Strand: Reverse

Name: potI [H]

Synonym: mll2887

Alternate gene names: 13472554

Gene position: 2327230-2326430 (Counterclockwise)

Preceding gene: 13472555

Following gene: 13472553

Centisome position: 33.08

GC content: 60.92

Gene sequence:

>801_bases
ATGGCTAGCAACTTCTCCATCAAGCACCAGCCGGGCTTCACGACAATCGCGGCGACCTGCTTCATCGTGCTCTATCTGCC
CATCATCGTGCTGGTCGCCTACGCTTTCAATGCGGCGAGCTCGACATCCGAATGGGGTGGCTTTTCGCTGCGATGGTTCC
AGTCGGCATACCAGAACACGCAGGTCATCGACGCAACGCTGCGCTCGTTCCAGATCGGTGCCATCGCGGCGGTGCTGTCG
ACCATCTTCGCCACGATGGCCGCACTTGCCACCACGCGCACCGCTTCCTATCCCGGGCTCACCTTCAAATATGCGGCGAT
CAACCAGCCGCTGATGGTGCCTGAGATCGTCACCGGCGTGGCGCTGCTGATCTTCTTCTCGCGCATCAAGATCTTCACCG
GCTATTCCGGCCTCGGCTACCTGATCGCCGCGCATACGGCATTCTGCATTCCGTTCGCCTATCTGCCGATCCGGGCGCGT
CTGGAGAATATGGACCTGACGCTCGAGCGCGCCGCGGCCGATCTCTACGCGACCCCGTGGAAGACCTTCCGCCGCATCAC
GCTGCCGCTCTTGTGGCCCGGTATCCTGGCTGGCCTGATGCTGGCCTTCGTCATCTCGCTCGATGACGTCGTCATCACAG
AGTTCGTCAAATCGGGCGGCCAGGACACGCTGCCCACTTACATGCTCGGCCAGATCCGCCGTGGCGTCACACCCGAGATC
AACGCGATATCGACCGCTTTCCTGCTGCTTTCGGTCGCGATCGTCACGTTGTTTTTCTTCGTCAGCAGGAAACGAGACTG
A

Upstream 100 bases:

>100_bases
AACTGGCCGCTTGGCTCGGCGCTGTCGATCACGGTGATGGTCATCGTCATGCTGGCATTGCTTGCCTATGTGCGCAACGC
CGGCAAGTCAGGGGTGCGTC

Downstream 100 bases:

>100_bases
AACCAATAATGGGAGTTAGAACGATGAACTGGAAGACAACGGCGACCGCCATGGGGTTGGCGCTGCTCGCTTCGACGGGC
CTCGCCCGCGCCGAGGGCGT

Product: ABC transporter, permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MASNFSIKHQPGFTTIAATCFIVLYLPIIVLVAYAFNAASSTSEWGGFSLRWFQSAYQNTQVIDATLRSFQIGAIAAVLS
TIFATMAALATTRTASYPGLTFKYAAINQPLMVPEIVTGVALLIFFSRIKIFTGYSGLGYLIAAHTAFCIPFAYLPIRAR
LENMDLTLERAAADLYATPWKTFRRITLPLLWPGILAGLMLAFVISLDDVVITEFVKSGGQDTLPTYMLGQIRRGVTPEI
NAISTAFLLLSVAIVTLFFFVSRKRD

Sequences:

>Translated_266_residues
MASNFSIKHQPGFTTIAATCFIVLYLPIIVLVAYAFNAASSTSEWGGFSLRWFQSAYQNTQVIDATLRSFQIGAIAAVLS
TIFATMAALATTRTASYPGLTFKYAAINQPLMVPEIVTGVALLIFFSRIKIFTGYSGLGYLIAAHTAFCIPFAYLPIRAR
LENMDLTLERAAADLYATPWKTFRRITLPLLWPGILAGLMLAFVISLDDVVITEFVKSGGQDTLPTYMLGQIRRGVTPEI
NAISTAFLLLSVAIVTLFFFVSRKRD
>Mature_265_residues
ASNFSIKHQPGFTTIAATCFIVLYLPIIVLVAYAFNAASSTSEWGGFSLRWFQSAYQNTQVIDATLRSFQIGAIAAVLST
IFATMAALATTRTASYPGLTFKYAAINQPLMVPEIVTGVALLIFFSRIKIFTGYSGLGYLIAAHTAFCIPFAYLPIRARL
ENMDLTLERAAADLYATPWKTFRRITLPLLWPGILAGLMLAFVISLDDVVITEFVKSGGQDTLPTYMLGQIRRGVTPEIN
AISTAFLLLSVAIVTLFFFVSRKRD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=256, Percent_Identity=36.328125, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1787368, Length=231, Percent_Identity=30.3030303030303, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787714, Length=263, Percent_Identity=31.5589353612167, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788764, Length=162, Percent_Identity=31.4814814814815, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787713, Length=132, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29212; Mature: 29080

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNFSIKHQPGFTTIAATCFIVLYLPIIVLVAYAFNAASSTSEWGGFSLRWFQSAYQNT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
QVIDATLRSFQIGAIAAVLSTIFATMAALATTRTASYPGLTFKYAAINQPLMVPEIVTGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCHHHHHHHH
ALLIFFSRIKIFTGYSGLGYLIAAHTAFCIPFAYLPIRARLENMDLTLERAAADLYATPW
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
KTFRRITLPLLWPGILAGLMLAFVISLDDVVITEFVKSGGQDTLPTYMLGQIRRGVTPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCH
NAISTAFLLLSVAIVTLFFFVSRKRD
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASNFSIKHQPGFTTIAATCFIVLYLPIIVLVAYAFNAASSTSEWGGFSLRWFQSAYQNT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
QVIDATLRSFQIGAIAAVLSTIFATMAALATTRTASYPGLTFKYAAINQPLMVPEIVTGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCHHHHHHHH
ALLIFFSRIKIFTGYSGLGYLIAAHTAFCIPFAYLPIRARLENMDLTLERAAADLYATPW
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
KTFRRITLPLLWPGILAGLMLAFVISLDDVVITEFVKSGGQDTLPTYMLGQIRRGVTPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCH
NAISTAFLLLSVAIVTLFFFVSRKRD
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]