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The map label for this gene is potD-A [H]

Identifier: 13472553

GI number: 13472553

Start: 2325372

End: 2326406

Strand: Reverse

Name: potD-A [H]

Synonym: mll2885

Alternate gene names: 13472553

Gene position: 2326406-2325372 (Counterclockwise)

Preceding gene: 13472554

Following gene: 13472552

Centisome position: 33.06

GC content: 59.81

Gene sequence:

>1035_bases
ATGAACTGGAAGACAACGGCGACCGCCATGGGGTTGGCGCTGCTCGCTTCGACGGGCCTCGCCCGCGCCGAGGGCGTGCT
GAACATCTACAATTGGGGCAACTACACCAGCCCCGACGTGATCAAGAAGTTCGAAGACAAATACAACATCAAGGTCACCA
TCACCGACTACGATTCCAACGACACCGCGCTCGCCAAGATCCGGCAGGGCGGCACCGGCTTCGACATTGCCGTGCCGTCG
CAAACCTACGTGCCGATCTGGATCAAGGAGGGCCTCCTCCTAGAGACCGATCCGGGCAAGATGGAGAACTTCAAGAACGT
CGCGCCAGAATGGGCCAATCCTGAATTCGATCCGGGCCGCAAATATTCGGTGCCGTGGGCCTGGGGCACGATTGGCGTCG
TCGTCAACACCGATGCCTACAAGGGTCCGGCCGATACGTGGGGCATCGTCTTCAACACGCCCGACGAGTTGAAGGGCAAG
GTCAACGTCGTTCCCGAGATGAACGACGTCATCTTCGCGGCGATCAAATATGTCGGCGGCCAGCAATGCACCGACGACAA
GGCGGTGCTGAAGAAGGTGCGCGACCTGCTGGTGGCGGCCAAGCCGAACTGGATCGCGATGGAATACAACACCATCGAGA
AGATGGGCGCCGGCGACTTCAAGGCAACCAGCGACTGGAACGGCTCGGCGCTGCGCCAGCGTCTGGCCAATGCGGCCATC
CATTACAACTATCCGAAGGAAGGCTTTGGCCTGTGGAGCGACAACGTCGTCGTCCTGAAGGAAGCCAAGAACGTCGAGAA
CGCCAAGCTGTTCCAGAACTTCATCATGGATCCGGAAAACGCGGCTGGCCTCTCGGCCTTCCACCGCTATGCCAACGCCA
TCACCGGCTCGGACAAATACATGCCGGCCGACATGAAGGACGCGCCGGAAGTCGTCATTCCGGCCGACGCCAAGCCGAAA
GGCGAGTTCCAGAAGATGTGCGCGCCGGAAACGCAGGAACTCTACACCAAGATCTGGACCGAACTGCAGAAGTAA

Upstream 100 bases:

>100_bases
CGATATCGACCGCTTTCCTGCTGCTTTCGGTCGCGATCGTCACGTTGTTTTTCTTCGTCAGCAGGAAACGAGACTGAAAC
CAATAATGGGAGTTAGAACG

Downstream 100 bases:

>100_bases
TCGCTGCATACCAGACCCGGCGGCACACACCGCCGGGTCTTTTCCTTCCGGGAATGCCTTTCATGGACTCCTATCGCAAC
AGCGATCCGCGGCCGCCGAT

Product: ABC transporter, periplasmic binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNWKTTATAMGLALLASTGLARAEGVLNIYNWGNYTSPDVIKKFEDKYNIKVTITDYDSNDTALAKIRQGGTGFDIAVPS
QTYVPIWIKEGLLLETDPGKMENFKNVAPEWANPEFDPGRKYSVPWAWGTIGVVVNTDAYKGPADTWGIVFNTPDELKGK
VNVVPEMNDVIFAAIKYVGGQQCTDDKAVLKKVRDLLVAAKPNWIAMEYNTIEKMGAGDFKATSDWNGSALRQRLANAAI
HYNYPKEGFGLWSDNVVVLKEAKNVENAKLFQNFIMDPENAAGLSAFHRYANAITGSDKYMPADMKDAPEVVIPADAKPK
GEFQKMCAPETQELYTKIWTELQK

Sequences:

>Translated_344_residues
MNWKTTATAMGLALLASTGLARAEGVLNIYNWGNYTSPDVIKKFEDKYNIKVTITDYDSNDTALAKIRQGGTGFDIAVPS
QTYVPIWIKEGLLLETDPGKMENFKNVAPEWANPEFDPGRKYSVPWAWGTIGVVVNTDAYKGPADTWGIVFNTPDELKGK
VNVVPEMNDVIFAAIKYVGGQQCTDDKAVLKKVRDLLVAAKPNWIAMEYNTIEKMGAGDFKATSDWNGSALRQRLANAAI
HYNYPKEGFGLWSDNVVVLKEAKNVENAKLFQNFIMDPENAAGLSAFHRYANAITGSDKYMPADMKDAPEVVIPADAKPK
GEFQKMCAPETQELYTKIWTELQK
>Mature_344_residues
MNWKTTATAMGLALLASTGLARAEGVLNIYNWGNYTSPDVIKKFEDKYNIKVTITDYDSNDTALAKIRQGGTGFDIAVPS
QTYVPIWIKEGLLLETDPGKMENFKNVAPEWANPEFDPGRKYSVPWAWGTIGVVVNTDAYKGPADTWGIVFNTPDELKGK
VNVVPEMNDVIFAAIKYVGGQQCTDDKAVLKKVRDLLVAAKPNWIAMEYNTIEKMGAGDFKATSDWNGSALRQRLANAAI
HYNYPKEGFGLWSDNVVVLKEAKNVENAKLFQNFIMDPENAAGLSAFHRYANAITGSDKYMPADMKDAPEVVIPADAKPK
GEFQKMCAPETQELYTKIWTELQK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=27.3480662983425, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787367, Length=328, Percent_Identity=28.9634146341463, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38170; Mature: 38170

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKTTATAMGLALLASTGLARAEGVLNIYNWGNYTSPDVIKKFEDKYNIKVTITDYDSN
CCCCHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHCCCEEEEEEEEECCCC
DTALAKIRQGGTGFDIAVPSQTYVPIWIKEGLLLETDPGKMENFKNVAPEWANPEFDPGR
CHHHHHHHCCCCCEEEECCCCCEEEEEEECCEEEECCCCHHHHHHHCCCCCCCCCCCCCC
KYSVPWAWGTIGVVVNTDAYKGPADTWGIVFNTPDELKGKVNVVPEMNDVIFAAIKYVGG
EEECCEECCEEEEEEECCCCCCCCCCCEEEECCCHHHCCCEEECCCHHHHHHHHHHHHCC
QQCTDDKAVLKKVRDLLVAAKPNWIAMEYNTIEKMGAGDFKATSDWNGSALRQRLANAAI
CCCCCHHHHHHHHHHHHEECCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHCEE
HYNYPKEGFGLWSDNVVVLKEAKNVENAKLFQNFIMDPENAAGLSAFHRYANAITGSDKY
EECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
MPADMKDAPEVVIPADAKPKGEFQKMCAPETQELYTKIWTELQK
CCCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNWKTTATAMGLALLASTGLARAEGVLNIYNWGNYTSPDVIKKFEDKYNIKVTITDYDSN
CCCCHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHCCCEEEEEEEEECCCC
DTALAKIRQGGTGFDIAVPSQTYVPIWIKEGLLLETDPGKMENFKNVAPEWANPEFDPGR
CHHHHHHHCCCCCEEEECCCCCEEEEEEECCEEEECCCCHHHHHHHCCCCCCCCCCCCCC
KYSVPWAWGTIGVVVNTDAYKGPADTWGIVFNTPDELKGKVNVVPEMNDVIFAAIKYVGG
EEECCEECCEEEEEEECCCCCCCCCCCEEEECCCHHHCCCEEECCCHHHHHHHHHHHHCC
QQCTDDKAVLKKVRDLLVAAKPNWIAMEYNTIEKMGAGDFKATSDWNGSALRQRLANAAI
CCCCCHHHHHHHHHHHHEECCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHCEE
HYNYPKEGFGLWSDNVVVLKEAKNVENAKLFQNFIMDPENAAGLSAFHRYANAITGSDKY
EECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
MPADMKDAPEVVIPADAKPKGEFQKMCAPETQELYTKIWTELQK
CCCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]