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The map label for this gene is potB [H]

Identifier: 13472555

GI number: 13472555

Start: 2327223

End: 2328143

Strand: Reverse

Name: potB [H]

Synonym: mll2888

Alternate gene names: 13472555

Gene position: 2328143-2327223 (Counterclockwise)

Preceding gene: 13472556

Following gene: 13472554

Centisome position: 33.09

GC content: 60.04

Gene sequence:

>921_bases
ATGGCCACCGCGGAAGAAGTCGCCAAGGCAGCGGAACGGCGTGATGTCCGTGACCGTTGGCTGTTGTCAGCACCGGCGCT
GCTGGTCATCCTGCTCGCCGCGACCGGCCCGTTGCTGATCGTGCTCGTCTACTCGTTCCTGACGCCCGGCGCCTATGGCG
ACGTGAAGTGGCAGTTTTCGCCCGATGCCTGGATATCGGTGTTCATGGAACGTGACATTTTCGACGACACGCTTTCGCTT
GCGGCGGCGCATGTCACCATCTTCTGGCGTTCGATCAAGCTTGCGGTGGTGACGACGCTCGCCACTTTGGCGCTCGGTTT
CCCGACCGCCTATTTCATGGCGACGCGCAGCGAGAAGACCAGGGATCTCTGGCTGTTCCTGATCACTATCCCGTTCTGGA
CCAACCTTCTGATCCGCACCTTCGCGGTTCTGCAGATCATCCGCAATGAAGGCATCATCAACACGATCCTGCTCAAGCTC
GGCATCATCTCGGCGCCGATCCAGATCCTCTACACCGACACCGCGATCCTGATCGGCATGGCCTATGTCTACCTGCCGCT
GATGGTGCTGCCGATCTATGCCAGCATGGAGAAGCTTGATTTCCGCCTGGTCGAGGCCGGCTATGATCTCTACGCGACAC
GCTTCAAGGTGCTGCGGAAGATCATTTTCCCGCTGGTCAAACCCGGCGTCATCGCCGGCTCGATCCTGGTCTTCATTCCG
GCACTCGGCGCTTATGTGACGCCGGTCGTGCTTGGCGGCGGCAAGAACATGATGCTGTCCAACCTGATCGAACTGCAGTT
CGGACAAGGCCGCAACTGGCCGCTTGGCTCGGCGCTGTCGATCACGGTGATGGTCATCGTCATGCTGGCATTGCTTGCCT
ATGTGCGCAACGCCGGCAAGTCAGGGGTGCGTCATGGCTAG

Upstream 100 bases:

>100_bases
TGCGCCAGCAGAACACGCGCAGCGCAGGCTGCGGTTTCGATCGCGGCGACAAGGTCGGCATCCTGATCGGCAACGACGCC
GCGCAAGTGCTGAGGGACTG

Downstream 100 bases:

>100_bases
CAACTTCTCCATCAAGCACCAGCCGGGCTTCACGACAATCGCGGCGACCTGCTTCATCGTGCTCTATCTGCCCATCATCG
TGCTGGTCGCCTACGCTTTC

Product: ABC transporter, permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MATAEEVAKAAERRDVRDRWLLSAPALLVILLAATGPLLIVLVYSFLTPGAYGDVKWQFSPDAWISVFMERDIFDDTLSL
AAAHVTIFWRSIKLAVVTTLATLALGFPTAYFMATRSEKTRDLWLFLITIPFWTNLLIRTFAVLQIIRNEGIINTILLKL
GIISAPIQILYTDTAILIGMAYVYLPLMVLPIYASMEKLDFRLVEAGYDLYATRFKVLRKIIFPLVKPGVIAGSILVFIP
ALGAYVTPVVLGGGKNMMLSNLIELQFGQGRNWPLGSALSITVMVIVMLALLAYVRNAGKSGVRHG

Sequences:

>Translated_306_residues
MATAEEVAKAAERRDVRDRWLLSAPALLVILLAATGPLLIVLVYSFLTPGAYGDVKWQFSPDAWISVFMERDIFDDTLSL
AAAHVTIFWRSIKLAVVTTLATLALGFPTAYFMATRSEKTRDLWLFLITIPFWTNLLIRTFAVLQIIRNEGIINTILLKL
GIISAPIQILYTDTAILIGMAYVYLPLMVLPIYASMEKLDFRLVEAGYDLYATRFKVLRKIIFPLVKPGVIAGSILVFIP
ALGAYVTPVVLGGGKNMMLSNLIELQFGQGRNWPLGSALSITVMVIVMLALLAYVRNAGKSGVRHG
>Mature_305_residues
ATAEEVAKAAERRDVRDRWLLSAPALLVILLAATGPLLIVLVYSFLTPGAYGDVKWQFSPDAWISVFMERDIFDDTLSLA
AAHVTIFWRSIKLAVVTTLATLALGFPTAYFMATRSEKTRDLWLFLITIPFWTNLLIRTFAVLQIIRNEGIINTILLKLG
IISAPIQILYTDTAILIGMAYVYLPLMVLPIYASMEKLDFRLVEAGYDLYATRFKVLRKIIFPLVKPGVIAGSILVFIPA
LGAYVTPVVLGGGKNMMLSNLIELQFGQGRNWPLGSALSITVMVIVMLALLAYVRNAGKSGVRHG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=260, Percent_Identity=40.3846153846154, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787080, Length=232, Percent_Identity=38.7931034482759, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=27.7056277056277, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33839; Mature: 33708

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAEEVAKAAERRDVRDRWLLSAPALLVILLAATGPLLIVLVYSFLTPGAYGDVKWQFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEC
PDAWISVFMERDIFDDTLSLAAAHVTIFWRSIKLAVVTTLATLALGFPTAYFMATRSEKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCC
RDLWLFLITIPFWTNLLIRTFAVLQIIRNEGIINTILLKLGIISAPIQILYTDTAILIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHH
AYVYLPLMVLPIYASMEKLDFRLVEAGYDLYATRFKVLRKIIFPLVKPGVIAGSILVFIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ALGAYVTPVVLGGGKNMMLSNLIELQFGQGRNWPLGSALSITVMVIVMLALLAYVRNAGK
HHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SGVRHG
CCCCCC
>Mature Secondary Structure 
ATAEEVAKAAERRDVRDRWLLSAPALLVILLAATGPLLIVLVYSFLTPGAYGDVKWQFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEC
PDAWISVFMERDIFDDTLSLAAAHVTIFWRSIKLAVVTTLATLALGFPTAYFMATRSEKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCC
RDLWLFLITIPFWTNLLIRTFAVLQIIRNEGIINTILLKLGIISAPIQILYTDTAILIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHH
AYVYLPLMVLPIYASMEKLDFRLVEAGYDLYATRFKVLRKIIFPLVKPGVIAGSILVFIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ALGAYVTPVVLGGGKNMMLSNLIELQFGQGRNWPLGSALSITVMVIVMLALLAYVRNAGK
HHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SGVRHG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]