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The map label for this gene is murE

Identifier: 13471554

GI number: 13471554

Start: 1299713

End: 1301167

Strand: Reverse

Name: murE

Synonym: mll1560

Alternate gene names: 13471554

Gene position: 1301167-1299713 (Counterclockwise)

Preceding gene: 13471555

Following gene: 13471553

Centisome position: 18.49

GC content: 66.25

Gene sequence:

>1455_bases
ATGAAGCTCAGAGATCTAGCCGGTGTCCTGCCAGTCGAAGGAACAGCTTCCGCCGATCTGGAGGTTACCGGGATTTCGTC
CGATTCCCGCCAGGTAAAACCGGGCGTCGTCTTTTTTGCGCTCGCCGGCACCAAGGCGGACGGTGCGGCCTACGCTGCCG
ACGCAGCCAGGCGCGGCGCGCTGGCGGTCGTGGCAGGCAAAAGCAATTCCGTTGCCGGACTGCCGGTTCCAGTGCTGACA
GTCGACGATCCGCGCCTGGCGCTGGCGCTGAGTGCTGCCCGCTATTTCGGGAAGCAGCCGCGGACTATGGTGGCTGTGAC
CGGCACCAGCGGCAAGACCTCGGTCGCCGCCTTCACCAGGCAGATCTGGGAGCAGGCGGGTTATGCGGCGGCGTCGATCG
GCACCACCGGCGTGGTGGCGCCCGGCCGCAACGAGTATGGCTCGTTGACCACGCCCGATCCGGTCGCCCTGCACCAATTG
CTGCGGGAATTGGCCGATGCCGGCGTCACCTATGCTTCGATGGAAGCCTCCAGCCATGGGCTCGACCAGCGCCGCCTCGA
CGGCGTGAAGCTCGCAGCCGGCGGCTTCACCAATCTCGGCCGCGACCACATGGATTACCATCCGACGGTCGAGGATTATC
ATCGCGCCAAGCTGCGCCTGTTCGACACGCTGCTGCCGAAGGGCGCGCCGGCGGTCATCTTCGCCGACGATCCGTGGTCG
GCGCCGACCATCCAGGCGGCACAGGCAGCCGGGCTCAATGTGCTGACGGTTGGCCGCCATGGCGATTTCCTCACGCTGAA
GCGGGTCGAGCACGAACGCCATCGCCAGCGCGCTGAGGTCGAGGCCGAGGGTGTGCTTTACGAAATCGACCTGCCGCTGG
CCGGCGATTTCCAGATTTCGAATGCGCTGGTCTCGGCGGGGCTTGCCATTTCAACTGGAACGCCTGTCGCCAAAGCATTG
GCCGCATTGGAAAAATTGAAGGGGGCGCCGGGCCGGCTCGATCTCGTCGGCACCACGGTCAGTGGTGCGCCGGTCTATGT
CGACTATGCCCACAAGCCCGATGCGCTGGAAAACGTGCTGGCTTCGGTGCGCCCGTTCACCACCGGCCGCGTCATGGTCG
TGTTCGGCTGCGGCGGCGACCGCGACCGGGGCAAAAGGCCGATCATGGGCGAGATCGCGACCCGGCTTGCCGATGTCGTC
ATCGTCACCGACGACAATCCGCGCTCCGAAGTGCCCGAGACGATCCGCGCCGCCATTCTGGCTGCTGCCCCGGGCGCCAT
CGAAATCGGCGACCGGCGCAAGGCGATCCACGAGGCTGTCGGCATGCTTCATGCCGGCGACACGCTGATCGTCGCCGGCA
AGGGGCACGAGGAAGGCCAGACGATCGGCTCCGAAACCTTGCATTTCTCCGACCACGAGGAAGTCCGCGCGGCGCTGCGG
GAGCGTGCCGCATGA

Upstream 100 bases:

>100_bases
AGATTTCAGCCATGAAAATGGTGCAACGTTGGTTTCCTATGAGTGATTCTTAGGGCGCGCCGGCGACTGCGCGCTATTTT
ATTGCGGTGAACGGAACTCG

Downstream 100 bases:

>100_bases
GTTTGCTGTGGACCTCCGACGCGCTGGTTGCCGCCATGGACGGGCGGCCGCTCGGGCCTATGCCCGAGGGCATATCGGGC
ATTTCGATCGACAGCCGCAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKLRDLAGVLPVEGTASADLEVTGISSDSRQVKPGVVFFALAGTKADGAAYAADAARRGALAVVAGKSNSVAGLPVPVLT
VDDPRLALALSAARYFGKQPRTMVAVTGTSGKTSVAAFTRQIWEQAGYAAASIGTTGVVAPGRNEYGSLTTPDPVALHQL
LRELADAGVTYASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDPWS
APTIQAAQAAGLNVLTVGRHGDFLTLKRVEHERHRQRAEVEAEGVLYEIDLPLAGDFQISNALVSAGLAISTGTPVAKAL
AALEKLKGAPGRLDLVGTTVSGAPVYVDYAHKPDALENVLASVRPFTTGRVMVVFGCGGDRDRGKRPIMGEIATRLADVV
IVTDDNPRSEVPETIRAAILAAAPGAIEIGDRRKAIHEAVGMLHAGDTLIVAGKGHEEGQTIGSETLHFSDHEEVRAALR
ERAA

Sequences:

>Translated_484_residues
MKLRDLAGVLPVEGTASADLEVTGISSDSRQVKPGVVFFALAGTKADGAAYAADAARRGALAVVAGKSNSVAGLPVPVLT
VDDPRLALALSAARYFGKQPRTMVAVTGTSGKTSVAAFTRQIWEQAGYAAASIGTTGVVAPGRNEYGSLTTPDPVALHQL
LRELADAGVTYASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDPWS
APTIQAAQAAGLNVLTVGRHGDFLTLKRVEHERHRQRAEVEAEGVLYEIDLPLAGDFQISNALVSAGLAISTGTPVAKAL
AALEKLKGAPGRLDLVGTTVSGAPVYVDYAHKPDALENVLASVRPFTTGRVMVVFGCGGDRDRGKRPIMGEIATRLADVV
IVTDDNPRSEVPETIRAAILAAAPGAIEIGDRRKAIHEAVGMLHAGDTLIVAGKGHEEGQTIGSETLHFSDHEEVRAALR
ERAA
>Mature_484_residues
MKLRDLAGVLPVEGTASADLEVTGISSDSRQVKPGVVFFALAGTKADGAAYAADAARRGALAVVAGKSNSVAGLPVPVLT
VDDPRLALALSAARYFGKQPRTMVAVTGTSGKTSVAAFTRQIWEQAGYAAASIGTTGVVAPGRNEYGSLTTPDPVALHQL
LRELADAGVTYASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDPWS
APTIQAAQAAGLNVLTVGRHGDFLTLKRVEHERHRQRAEVEAEGVLYEIDLPLAGDFQISNALVSAGLAISTGTPVAKAL
AALEKLKGAPGRLDLVGTTVSGAPVYVDYAHKPDALENVLASVRPFTTGRVMVVFGCGGDRDRGKRPIMGEIATRLADVV
IVTDDNPRSEVPETIRAAILAAAPGAIEIGDRRKAIHEAVGMLHAGDTLIVAGKGHEEGQTIGSETLHFSDHEEVRAALR
ERAA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=489, Percent_Identity=35.9918200408998, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786274, Length=319, Percent_Identity=27.8996865203762, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_RHILO (Q98KA8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103120.1
- ProteinModelPortal:   Q98KA8
- SMR:   Q98KA8
- GeneID:   1225783
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1560
- NMPDR:   fig|266835.1.peg.1225
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   PRK00139
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 50751; Mature: 50751

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 177-177 BINDING 183-183 BINDING 185-185 BINDING 381-381 BINDING 453-453 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRDLAGVLPVEGTASADLEVTGISSDSRQVKPGVVFFALAGTKADGAAYAADAARRGA
CCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCC
LAVVAGKSNSVAGLPVPVLTVDDPRLALALSAARYFGKQPRTMVAVTGTSGKTSVAAFTR
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWEQAGYAAASIGTTGVVAPGRNEYGSLTTPDPVALHQLLRELADAGVTYASMEASSHG
HHHHHCCCEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
LDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDPWS
CCHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
APTIQAAQAAGLNVLTVGRHGDFLTLKRVEHERHRQRAEVEAEGVLYEIDLPLAGDFQIS
CCCHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
NALVSAGLAISTGTPVAKALAALEKLKGAPGRLDLVGTTVSGAPVYVDYAHKPDALENVL
HHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHHH
ASVRPFTTGRVMVVFGCGGDRDRGKRPIMGEIATRLADVVIVTDDNPRSEVPETIRAAIL
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHH
AAAPGAIEIGDRRKAIHEAVGMLHAGDTLIVAGKGHEEGQTIGSETLHFSDHEEVRAALR
HCCCCCEEECHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHH
ERAA
HHCC
>Mature Secondary Structure
MKLRDLAGVLPVEGTASADLEVTGISSDSRQVKPGVVFFALAGTKADGAAYAADAARRGA
CCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCC
LAVVAGKSNSVAGLPVPVLTVDDPRLALALSAARYFGKQPRTMVAVTGTSGKTSVAAFTR
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWEQAGYAAASIGTTGVVAPGRNEYGSLTTPDPVALHQLLRELADAGVTYASMEASSHG
HHHHHCCCEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
LDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDPWS
CCHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
APTIQAAQAAGLNVLTVGRHGDFLTLKRVEHERHRQRAEVEAEGVLYEIDLPLAGDFQIS
CCCHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
NALVSAGLAISTGTPVAKALAALEKLKGAPGRLDLVGTTVSGAPVYVDYAHKPDALENVL
HHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCHHHHHHH
ASVRPFTTGRVMVVFGCGGDRDRGKRPIMGEIATRLADVVIVTDDNPRSEVPETIRAAIL
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHH
AAAPGAIEIGDRRKAIHEAVGMLHAGDTLIVAGKGHEEGQTIGSETLHFSDHEEVRAALR
HCCCCCEEECHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHH
ERAA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968