| Definition | Mesorhizobium loti MAFF303099 chromosome, complete genome. |
|---|---|
| Accession | NC_002678 |
| Length | 7,036,071 |
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The map label for this gene is murF [H]
Identifier: 13471553
GI number: 13471553
Start: 1298283
End: 1299716
Strand: Reverse
Name: murF [H]
Synonym: mll1559
Alternate gene names: 13471553
Gene position: 1299716-1298283 (Counterclockwise)
Preceding gene: 13471554
Following gene: 13471552
Centisome position: 18.47
GC content: 66.32
Gene sequence:
>1434_bases ATGAGTTTGCTGTGGACCTCCGACGCGCTGGTTGCCGCCATGGACGGGCGGCCGCTCGGGCCTATGCCCGAGGGCATATC GGGCATTTCGATCGACAGCCGCAGCCTGCAGCCGGGTGACGCCTTCTTCGCCATCAAGGGCGAGGCGATGGATGGCCACG ACTTCGCCACCGCGGCCATCAAGGCCGGCGCCGGCGTGCTGGTGGTGGCGGAAGGCAAGCTGCCGTCGCTCGGCCGGCTG ACGGCGCCGATCATCGTCGTTGAGGACGTGCTGGTCGCGCTGGAAAAGCTCGGTGTGGCGGCGCGTGCCCGCTCGCAAGC CAAGATTATCGCGGTGACCGGTTCGGCCGGCAAGACCACCACCAAGGAAGCGCTGCGCCATGTGCTGTCGGCGGTCGGCA AGGTGCATGCTTCGGCACAGTCCTTCAACAACCATTGGGGGGTGCCGCTGACGCTCGCGCGCATGCCCGATAACTGCGAC TATGCCGTGTTCGAGATCGGCATGAACCATCCCGACGAGATCCGGCCGCTGGTGAAGATGGTGCGGCCGCATGTCGCCAT CGTCACCATGATCGCGGCGGCGCATCTCGGCTTCTTCCGCAATCTCGACGAGATCGCCAAGGCCAAGGCCGAGATTTTCG AGGGGCTGGAGCCCGAAGGCGCCGCCGTCCTCAACCGCGACGATGGCCGCTTCAAGCTGCTCGACAAGATGGCGCATGCC GCCGGCGTCGAGCATGTCTACGGCTTCGGCGAGAATGCGCGCTCGACCTTCAAGCTCACCAAATGCGAATTGCATGCCGA CCATTCCGACATCGCCGCGCGGATCGGCGGCCATGACATGATCGCCCGCATCGGCGCGCCTGGCCGCCACATGGTGCAGA ATGTGCTGGCGGTGCTGGGCGCGGCGCAGCTGGTCGGCGCCGATCTCAACAAGGTGGCCCTGGCACTGGCCGATCTCTCG GCGGAGCGCGGCCGCGGCAAGCGCCATGTGTTGCGCCACCCCGGTGGCCCGATCACGCTGATCGACGAGAGCTACAACGC CAATCCGGCCTCGATGGCGGCGGCCATGGCGCTGCTCAACGCGACGCCGGTGACGGGTGAGGGGCGCCGCATCGCCGTGC TCGGCGACATGCTGGAACTCGGCGATCACTCGGCCAAGCTGCATGCCGCCCTTGCCGACCTCATCGTCGGCACCGGCACG CAGACGGTCTTTCTCGGCGGCCCCGAAATGCGGGCGCTGGCGGAAGCGCTGCCGGGCGACATCAAGATGGAATACCGCGC CGGCGTCGAGCAATTGAAGCCGGTGCTGCTTGCCGCGCTGAAGCCTGGCGACGTGGTGATGATCAAATCGTCGAAGGGCA TCGGTTTTGCAAAACTGGTCGATGCGCTGCTGGGCAAGTACCCGGCTGAAACGACAACCAGCAAACTGACCTAG
Upstream 100 bases:
>100_bases TCGCCGGCAAGGGGCACGAGGAAGGCCAGACGATCGGCTCCGAAACCTTGCATTTCTCCGACCACGAGGAAGTCCGCGCG GCGCTGCGGGAGCGTGCCGC
Downstream 100 bases:
>100_bases GTCAAGTCGGGGGACGAAAGCGCATGTTTACACTGCTGGTCGATTTCGCGGACAAGATCTCGGTCTTCAATGTCTTCCGC TACATCACGTTCCGCACCGG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 477; Mature: 476
Protein sequence:
>477_residues MSLLWTSDALVAAMDGRPLGPMPEGISGISIDSRSLQPGDAFFAIKGEAMDGHDFATAAIKAGAGVLVVAEGKLPSLGRL TAPIIVVEDVLVALEKLGVAARARSQAKIIAVTGSAGKTTTKEALRHVLSAVGKVHASAQSFNNHWGVPLTLARMPDNCD YAVFEIGMNHPDEIRPLVKMVRPHVAIVTMIAAAHLGFFRNLDEIAKAKAEIFEGLEPEGAAVLNRDDGRFKLLDKMAHA AGVEHVYGFGENARSTFKLTKCELHADHSDIAARIGGHDMIARIGAPGRHMVQNVLAVLGAAQLVGADLNKVALALADLS AERGRGKRHVLRHPGGPITLIDESYNANPASMAAAMALLNATPVTGEGRRIAVLGDMLELGDHSAKLHAALADLIVGTGT QTVFLGGPEMRALAEALPGDIKMEYRAGVEQLKPVLLAALKPGDVVMIKSSKGIGFAKLVDALLGKYPAETTTSKLT
Sequences:
>Translated_477_residues MSLLWTSDALVAAMDGRPLGPMPEGISGISIDSRSLQPGDAFFAIKGEAMDGHDFATAAIKAGAGVLVVAEGKLPSLGRL TAPIIVVEDVLVALEKLGVAARARSQAKIIAVTGSAGKTTTKEALRHVLSAVGKVHASAQSFNNHWGVPLTLARMPDNCD YAVFEIGMNHPDEIRPLVKMVRPHVAIVTMIAAAHLGFFRNLDEIAKAKAEIFEGLEPEGAAVLNRDDGRFKLLDKMAHA AGVEHVYGFGENARSTFKLTKCELHADHSDIAARIGGHDMIARIGAPGRHMVQNVLAVLGAAQLVGADLNKVALALADLS AERGRGKRHVLRHPGGPITLIDESYNANPASMAAAMALLNATPVTGEGRRIAVLGDMLELGDHSAKLHAALADLIVGTGT QTVFLGGPEMRALAEALPGDIKMEYRAGVEQLKPVLLAALKPGDVVMIKSSKGIGFAKLVDALLGKYPAETTTSKLT >Mature_476_residues SLLWTSDALVAAMDGRPLGPMPEGISGISIDSRSLQPGDAFFAIKGEAMDGHDFATAAIKAGAGVLVVAEGKLPSLGRLT APIIVVEDVLVALEKLGVAARARSQAKIIAVTGSAGKTTTKEALRHVLSAVGKVHASAQSFNNHWGVPLTLARMPDNCDY AVFEIGMNHPDEIRPLVKMVRPHVAIVTMIAAAHLGFFRNLDEIAKAKAEIFEGLEPEGAAVLNRDDGRFKLLDKMAHAA GVEHVYGFGENARSTFKLTKCELHADHSDIAARIGGHDMIARIGAPGRHMVQNVLAVLGAAQLVGADLNKVALALADLSA ERGRGKRHVLRHPGGPITLIDESYNANPASMAAAMALLNATPVTGEGRRIAVLGDMLELGDHSAKLHAALADLIVGTGTQ TVFLGGPEMRALAEALPGDIKMEYRAGVEQLKPVLLAALKPGDVVMIKSSKGIGFAKLVDALLGKYPAETTTSKLT
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=35.0678733031674, Blast_Score=194, Evalue=1e-50,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 50079; Mature: 49948
Theoretical pI: Translated: 7.58; Mature: 7.58
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 3.6 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSLLWTSDALVAAMDGRPLGPMPEGISGISIDSRSLQPGDAFFAIKGEAMDGHDFATAAI CCEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHHH KAGAGVLVVAEGKLPSLGRLTAPIIVVEDVLVALEKLGVAARARSQAKIIAVTGSAGKTT HCCCCEEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCH TKEALRHVLSAVGKVHASAQSFNNHWGVPLTLARMPDNCDYAVFEIGMNHPDEIRPLVKM HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHH VRPHVAIVTMIAAAHLGFFRNLDEIAKAKAEIFEGLEPEGAAVLNRDDGRFKLLDKMAHA HCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHH AGVEHVYGFGENARSTFKLTKCELHADHSDIAARIGGHDMIARIGAPGRHMVQNVLAVLG HCHHHHHCCCCCCCCEEEEEEEEEECCHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHH AAQLVGADLNKVALALADLSAERGRGKRHVLRHPGGPITLIDESYNANPASMAAAMALLN HHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH ATPVTGEGRRIAVLGDMLELGDHSAKLHAALADLIVGTGTQTVFLGGPEMRALAEALPGD CCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCC IKMEYRAGVEQLKPVLLAALKPGDVVMIKSSKGIGFAKLVDALLGKYPAETTTSKLT EEEHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC >Mature Secondary Structure SLLWTSDALVAAMDGRPLGPMPEGISGISIDSRSLQPGDAFFAIKGEAMDGHDFATAAI CEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHHH KAGAGVLVVAEGKLPSLGRLTAPIIVVEDVLVALEKLGVAARARSQAKIIAVTGSAGKTT HCCCCEEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCH TKEALRHVLSAVGKVHASAQSFNNHWGVPLTLARMPDNCDYAVFEIGMNHPDEIRPLVKM HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHH VRPHVAIVTMIAAAHLGFFRNLDEIAKAKAEIFEGLEPEGAAVLNRDDGRFKLLDKMAHA HCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHH AGVEHVYGFGENARSTFKLTKCELHADHSDIAARIGGHDMIARIGAPGRHMVQNVLAVLG HCHHHHHCCCCCCCCEEEEEEEEEECCHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHH AAQLVGADLNKVALALADLSAERGRGKRHVLRHPGGPITLIDESYNANPASMAAAMALLN HHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH ATPVTGEGRRIAVLGDMLELGDHSAKLHAALADLIVGTGTQTVFLGGPEMRALAEALPGD CCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCC IKMEYRAGVEQLKPVLLAALKPGDVVMIKSSKGIGFAKLVDALLGKYPAETTTSKLT EEEHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11557893 [H]