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The map label for this gene is murD

Identifier: 13471551

GI number: 13471551

Start: 1295765

End: 1297165

Strand: Reverse

Name: murD

Synonym: mll1557

Alternate gene names: 13471551

Gene position: 1297165-1295765 (Counterclockwise)

Preceding gene: 13471552

Following gene: 13471550

Centisome position: 18.44

GC content: 66.24

Gene sequence:

>1401_bases
TTGATCCCCGCCGCATCCTTCGCAGGCAAACAGGTTTCGCTCTTCGGCCTCGGTGGCTCGGGGATCGCGACCGCGCGGGC
ATTGATCGAGGGTGGGGCGCAGGTGCTTGCCTGGGATGACAATCCCGACAGCGTCGCCAAGGCCGCCGCGACCGGCATCG
CAACCGCCGATCTGCGCGGCGCCGACTGGGCGAAATTCTCGGCCTTCGTGCTGTCGCCGGGCGTGCCGCTGACGCATCCC
AAGCCGCATTGGACGGTGGAATTGGCAAAGGGCGCCGGTGTCGAGGTGATCGGCGACATCGAATTGTTCTGCCGCGAGCG
GATTTTGCAGGCGCCGACGGCGCCGTTCATCGCCATCACCGGCACCAACGGCAAGTCGACGACGACGGCGCTGACGGCAC
ATATCCTCAAATCGGCAGGCCGCGACACCCAGATGGGCGGCAATATCGGCCGCGCGGTGATGACGCTCGATCCGCCGAAG
CCCGATCGCCACTTTGTCGTCGAGTGCTCGTCCTACCAGATCGACCTCGCGCCTTCGATCAACCCGACGGCGGGCATCCT
GCTCAATCTGACGCCGGACCATCTCGACCGCCACGGCACCATGCAGCACTACGCCTCGATCAAGGAGCGGCTGGTGGCGG
GCAGCGAGACGGCGATCATCGGCATCGACGATTCCTGGTGTGCGCAGATCGCAGAGCGGCTGGAACGGGCAGGGCAGCAG
GTCATCCGCATTTCCAAGCGGCTGCCGCTGACCGACGGCTATTTCGCCGACGGCACCAATCTGATGGAGGCCGTGCACGG
CCGCTACAGCAAGGTCGCCTTCCTCGAAGGCATCGGTTCGTTGCGCGGCCAGCACAATGCGCAGAACGCGCTTGCCGCGG
TCGCCGCTTGCCTCAAGGTCGGGCTCGACCTCGGCGAAATCCAGTCGGGGCTCGAAAGTTTTCCGGGCCTCGCGCACCGC
ATGGAGCAGGTCGGCCGCAAGGACCATGTCCTGTTCGTCAACGATTCCAAGGCGACCAACGCCGACGCGGCGGCGCCGGC
GCTGTCGAGCTTTCCGCGCATCTACTGGATCGCCGGCGGCCTGCCCAAGGAAGGCGGCATCGAGCCGCTGCGCGGCTTCT
TCCCGCGCATCGCCAAGGCCTATCTGATCGGCGAGGCCGCCCCTGCCTTTTCGGCGACGCTGGGCGAGGCGGTGCCCTAC
GAGATCTCAGGCACGCTGGCCGCGGCGGTCGCGCACGCAGCGCATGATGCGGCCAAGGACGACAGCGGCGAGGTGGTGGT
GCTGTTGTCGCCGGCCTGCGCCAGTTTCGACCAGTTCAAGAATTTCGAAGTTCGCGGCGAAGCCTTCAGGCAAGCTGCGA
GCGCTATAGATGGCGTCAAACCCATCGGAGGGGCACGATAA

Upstream 100 bases:

>100_bases
GCTGGACCGAGAGCCAGGTGGTGATCCGTTTCTGGATCATCGCCGTCATCCTGGCGCTGGTCGGCCTCTCCACCCTGAAG
CTCAGATAGGAAGCCCTCCC

Downstream 100 bases:

>100_bases
TGCAGAGCCGTCTCGATAAAAGTCCTGTCGCAACCTGGTGGTGGACGATCGATCGCTGGTTCCTGGCGGCGTTCCTGTCG
CTGATGGGGCTGGGCATCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIPAASFAGKQVSLFGLGGSGIATARALIEGGAQVLAWDDNPDSVAKAAATGIATADLRGADWAKFSAFVLSPGVPLTHP
KPHWTVELAKGAGVEVIGDIELFCRERILQAPTAPFIAITGTNGKSTTTALTAHILKSAGRDTQMGGNIGRAVMTLDPPK
PDRHFVVECSSYQIDLAPSINPTAGILLNLTPDHLDRHGTMQHYASIKERLVAGSETAIIGIDDSWCAQIAERLERAGQQ
VIRISKRLPLTDGYFADGTNLMEAVHGRYSKVAFLEGIGSLRGQHNAQNALAAVAACLKVGLDLGEIQSGLESFPGLAHR
MEQVGRKDHVLFVNDSKATNADAAAPALSSFPRIYWIAGGLPKEGGIEPLRGFFPRIAKAYLIGEAAPAFSATLGEAVPY
EISGTLAAAVAHAAHDAAKDDSGEVVVLLSPACASFDQFKNFEVRGEAFRQAASAIDGVKPIGGAR

Sequences:

>Translated_466_residues
MIPAASFAGKQVSLFGLGGSGIATARALIEGGAQVLAWDDNPDSVAKAAATGIATADLRGADWAKFSAFVLSPGVPLTHP
KPHWTVELAKGAGVEVIGDIELFCRERILQAPTAPFIAITGTNGKSTTTALTAHILKSAGRDTQMGGNIGRAVMTLDPPK
PDRHFVVECSSYQIDLAPSINPTAGILLNLTPDHLDRHGTMQHYASIKERLVAGSETAIIGIDDSWCAQIAERLERAGQQ
VIRISKRLPLTDGYFADGTNLMEAVHGRYSKVAFLEGIGSLRGQHNAQNALAAVAACLKVGLDLGEIQSGLESFPGLAHR
MEQVGRKDHVLFVNDSKATNADAAAPALSSFPRIYWIAGGLPKEGGIEPLRGFFPRIAKAYLIGEAAPAFSATLGEAVPY
EISGTLAAAVAHAAHDAAKDDSGEVVVLLSPACASFDQFKNFEVRGEAFRQAASAIDGVKPIGGAR
>Mature_466_residues
MIPAASFAGKQVSLFGLGGSGIATARALIEGGAQVLAWDDNPDSVAKAAATGIATADLRGADWAKFSAFVLSPGVPLTHP
KPHWTVELAKGAGVEVIGDIELFCRERILQAPTAPFIAITGTNGKSTTTALTAHILKSAGRDTQMGGNIGRAVMTLDPPK
PDRHFVVECSSYQIDLAPSINPTAGILLNLTPDHLDRHGTMQHYASIKERLVAGSETAIIGIDDSWCAQIAERLERAGQQ
VIRISKRLPLTDGYFADGTNLMEAVHGRYSKVAFLEGIGSLRGQHNAQNALAAVAACLKVGLDLGEIQSGLESFPGLAHR
MEQVGRKDHVLFVNDSKATNADAAAPALSSFPRIYWIAGGLPKEGGIEPLRGFFPRIAKAYLIGEAAPAFSATLGEAVPY
EISGTLAAAVAHAAHDAAKDDSGEVVVLLSPACASFDQFKNFEVRGEAFRQAASAIDGVKPIGGAR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=33.4056399132321, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_RHILO (Q98KB1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103117.1
- ProteinModelPortal:   Q98KB1
- SMR:   Q98KB1
- GeneID:   1225780
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1557
- NMPDR:   fig|266835.1.peg.1222
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK01390
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48855; Mature: 48855

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPAASFAGKQVSLFGLGGSGIATARALIEGGAQVLAWDDNPDSVAKAAATGIATADLRG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCC
ADWAKFSAFVLSPGVPLTHPKPHWTVELAKGAGVEVIGDIELFCRERILQAPTAPFIAIT
CCHHHHEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEE
GTNGKSTTTALTAHILKSAGRDTQMGGNIGRAVMTLDPPKPDRHFVVECSSYQIDLAPSI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGTMQHYASIKERLVAGSETAIIGIDDSWCAQIAERLERAGQQ
CCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
VIRISKRLPLTDGYFADGTNLMEAVHGRYSKVAFLEGIGSLRGQHNAQNALAAVAACLKV
HHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GLDLGEIQSGLESFPGLAHRMEQVGRKDHVLFVNDSKATNADAAAPALSSFPRIYWIAGG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHCHHHHHCCEEEEEECC
LPKEGGIEPLRGFFPRIAKAYLIGEAAPAFSATLGEAVPYEISGTLAAAVAHAAHDAAKD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCC
DSGEVVVLLSPACASFDQFKNFEVRGEAFRQAASAIDGVKPIGGAR
CCCCEEEEECCCHHCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIPAASFAGKQVSLFGLGGSGIATARALIEGGAQVLAWDDNPDSVAKAAATGIATADLRG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCC
ADWAKFSAFVLSPGVPLTHPKPHWTVELAKGAGVEVIGDIELFCRERILQAPTAPFIAIT
CCHHHHEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEE
GTNGKSTTTALTAHILKSAGRDTQMGGNIGRAVMTLDPPKPDRHFVVECSSYQIDLAPSI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGTMQHYASIKERLVAGSETAIIGIDDSWCAQIAERLERAGQQ
CCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
VIRISKRLPLTDGYFADGTNLMEAVHGRYSKVAFLEGIGSLRGQHNAQNALAAVAACLKV
HHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GLDLGEIQSGLESFPGLAHRMEQVGRKDHVLFVNDSKATNADAAAPALSSFPRIYWIAGG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHCHHHHHCCEEEEEECC
LPKEGGIEPLRGFFPRIAKAYLIGEAAPAFSATLGEAVPYEISGTLAAAVAHAAHDAAKD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCC
DSGEVVVLLSPACASFDQFKNFEVRGEAFRQAASAIDGVKPIGGAR
CCCCEEEEECCCHHCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968