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The map label for this gene is miaA

Identifier: 13471468

GI number: 13471468

Start: 1208007

End: 1208972

Strand: Reverse

Name: miaA

Synonym: mll1448

Alternate gene names: 13471468

Gene position: 1208972-1208007 (Counterclockwise)

Preceding gene: 13471471

Following gene: 13471467

Centisome position: 17.18

GC content: 64.29

Gene sequence:

>966_bases
ATGAGCGGCATCGAGAATTCCGGCGCGGATGCGGGCGAAGGCCGCGTGAAGAATGCGATCCTGATAGCCGGGCCAACCGC
CAGCGGCAAGTCGGCACTGGCGCTGGACTTGGCCGAGCGCGCAGGCGGCGTCATCGTTAACAGCGATTCCATGCAGGGCT
ATTCGGTGCTCGACGTGCTGACCGCCCGGCCGGAAGCGGCCGATCTCGCACGCGTGCCGCATTTTCTCTACGGCCATGTC
CATCCCGGCACCGCCTATTCGACCGGCGCATGGCTGCGTGACGTGATGAAACTCATCGATGACGGCATGCTGTCGCGGCG
GCCGGTCTTCGTTGGTGGTACCGGGCTTTACTTCCGCGCGCTGGCCGAGGGTATTTCGGAAATGCCCGACATTCCGCCGC
GCATTCGCGACCGCTGGCGCTACGAGCTGAAAGAGCAAGGCGCGGTCAAGCTGCACGGCCTGCTGCTGCGCGAGGATTCG
AGAGCCGCCATGCAGCTCAAGCCAACCGACAGCCAGCGCATCGTCCGGGCGCTCGAAGTGCTCGACGCCTCCGGCCGATC
GATCCTGGAATGGCAAGCGGAGCGCGGCCGGCCGCTCATCGACAGGCAAACGGCGCGTTTCCTGGTCATCGAGCCGGACC
GGGCCGCGCTGGTCGATCGCATCGAAAGGCGCTTCGACCAGATGCTGGACAAGGGTGCGCTGGAGGAAGTCAGGCAACTT
GCGGCCCTTGGCCTTGATCCAGATTTGCCGGCTATGAAGGCGATCGGCGTTCGCGACCTGCAGGCGGCGATGGCGGGACA
GCTGAGCTTTCCCGAGGCGATCGAGCGCGCCAAGATCGCAACCCGGCAATACGCCAAGCGGCAGGCGACTTGGTTTCGCC
ATCAGCTGGGGCCGGAATGGCAGAGATTGCGGCCCGGTGACGATCTTGAAACCACGATGCAAACACTTGTTGCTAATGCA
ACTTAA

Upstream 100 bases:

>100_bases
GGCCAGCCAGACGACAGTGCTTGCGCCCACCGAACGCGAGGCCATATTCGCAAGTGACGGCGACAGAGCGCGGTCAGCGG
GACGGGAAACAAGCGTGGCA

Downstream 100 bases:

>100_bases
AAAGTTGACCATGACGGAAGGGTTCGCGCCGAAGTTGCCGGGATTAACGCTCCGTAAGGCGGTCCGTGCCATAAGGTCGA
TGGGCACTTTTCCATTGGGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSGIENSGADAGEGRVKNAILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVLTARPEAADLARVPHFLYGHV
HPGTAYSTGAWLRDVMKLIDDGMLSRRPVFVGGTGLYFRALAEGISEMPDIPPRIRDRWRYELKEQGAVKLHGLLLREDS
RAAMQLKPTDSQRIVRALEVLDASGRSILEWQAERGRPLIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQL
AALGLDPDLPAMKAIGVRDLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEWQRLRPGDDLETTMQTLVANA
T

Sequences:

>Translated_321_residues
MSGIENSGADAGEGRVKNAILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVLTARPEAADLARVPHFLYGHV
HPGTAYSTGAWLRDVMKLIDDGMLSRRPVFVGGTGLYFRALAEGISEMPDIPPRIRDRWRYELKEQGAVKLHGLLLREDS
RAAMQLKPTDSQRIVRALEVLDASGRSILEWQAERGRPLIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQL
AALGLDPDLPAMKAIGVRDLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEWQRLRPGDDLETTMQTLVANA
T
>Mature_320_residues
SGIENSGADAGEGRVKNAILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVLTARPEAADLARVPHFLYGHVH
PGTAYSTGAWLRDVMKLIDDGMLSRRPVFVGGTGLYFRALAEGISEMPDIPPRIRDRWRYELKEQGAVKLHGLLLREDSR
AAMQLKPTDSQRIVRALEVLDASGRSILEWQAERGRPLIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQLA
ALGLDPDLPAMKAIGVRDLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEWQRLRPGDDLETTMQTLVANAT

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=27.8846153846154, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=39.4557823129252, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=24.5847176079734, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=28.5234899328859, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=26.0479041916168, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RHILO (Q98KJ4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103034.1
- ProteinModelPortal:   Q98KJ4
- SMR:   Q98KJ4
- GeneID:   1225697
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1448
- NMPDR:   fig|266835.1.peg.1139
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35411; Mature: 35280

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIENSGADAGEGRVKNAILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVL
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEHHHHCCCEEEECCCCCCHHHHHHH
TARPEAADLARVPHFLYGHVHPGTAYSTGAWLRDVMKLIDDGMLSRRPVFVGGTGLYFRA
HCCCCHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
LAEGISEMPDIPPRIRDRWRYELKEQGAVKLHGLLLREDSRAAMQLKPTDSQRIVRALEV
HHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEHEEEECCCCCEEEECCCCHHHHHHHHHH
LDASGRSILEWQAERGRPLIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQL
HHCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALGLDPDLPAMKAIGVRDLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEW
HHHCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QRLRPGDDLETTMQTLVANAT
HHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGIENSGADAGEGRVKNAILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEHHHHCCCEEEECCCCCCHHHHHHH
TARPEAADLARVPHFLYGHVHPGTAYSTGAWLRDVMKLIDDGMLSRRPVFVGGTGLYFRA
HCCCCHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHH
LAEGISEMPDIPPRIRDRWRYELKEQGAVKLHGLLLREDSRAAMQLKPTDSQRIVRALEV
HHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEHEEEECCCCCEEEECCCCHHHHHHHHHH
LDASGRSILEWQAERGRPLIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQL
HHCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALGLDPDLPAMKAIGVRDLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEW
HHHCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QRLRPGDDLETTMQTLVANAT
HHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968