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The map label for this gene is speB [C]

Identifier: 13470645

GI number: 13470645

Start: 297466

End: 298398

Strand: Reverse

Name: speB [C]

Synonym: mll0412

Alternate gene names: 13470645

Gene position: 298398-297466 (Counterclockwise)

Preceding gene: 13470648

Following gene: 13470639

Centisome position: 4.24

GC content: 65.92

Gene sequence:

>933_bases
ATGACCGCCTCACCCTCCTTTCTCGGCCTTCCCGATCGCCTGGACGGCCGCACGCCCCGTGCGGTGATCTTTGGGGCCGG
CCACGGCAGCACCTATCCCGGCAAGGACAGCAGCGGCCATGCGTTGGCGGCCGACGCCATTCGTGCGGCCAGCCAGGATG
ATGCCTCGCTTGTCGAGCACTGGGATTTCGATCTCGGTGGCCCGTTGTTCAACGGCAAGCCGGCCTCTTGCGTCGACGCC
GGCGACATCTCGACCATCCTGCACGACAATGCCGAAAACAGGGTCCGGATCGAAGCGAAGACGCGCGAGGTCCTGGCCTT
GCCGGCCGTGCCGATCCTGCTTGGCGGTGACGATTCCGTCGTCATTCCGTTCCTTGCCGGCTTTGCCGATCACGGACCGA
TCTGGGTCCTGCAGATCGATGCCCATATCGACTGGCGCGACGAGGTGCTTGGCGAACGCCACGGCTATTCGAGCCCGATG
CGAAGGGCAAGCGAGATGCCGCATGTCGCCGGCATAGTGCAGGTTGGCCTGCGCAGCGTCGGCAGCGCGCGCCTCGCTGA
AATCGAAGCCGCGCGGCACTATGGCAGCCGTTTCGTGACAGCGCGCGAGGTTCATGCTCAAGGTGTGGATGCAGCGCTCA
GGCATATTCCGGAAGGCGCGCGGGTCGTCGCCACCTTCGACTGCGACAGCCTTGATCCCGGCATCATGCCGGGCGTGGCG
GCGCGTACGCCGGGCGGCCTCACCTACACGCAAGCGATCGACCTGATCGCCGGCCTCGGCAAGCGGGCCAGGATCGCGGG
ATTCGACCTTGTCGAGCTCTATCCGCCCGCCGACATAGACGGCCTGTCAGCGCTGACCGCTGCGCGCCTTCTCGTCAATG
TGATCGGCACCATCGCCAGCATGACGGTATCGCAGTCGTCCAGCACCGCCTGA

Upstream 100 bases:

>100_bases
TTTGACCCATGCTCCCTGGCCCGCCTGCGATTTTCGGTGATGGCATCGAAGCGGCGAAGCAACTAGCCTGAGGTAGCGCT
GGAACTCAGGACCATCAAGA

Downstream 100 bases:

>100_bases
TTGTCGGCGGCAACGCGTATATTCGGATAGCGGGAAGCGAGGTTCGCCGCGATCTCTTCGTTGCGCGGCGACAACACCAC
CGATACCGGATCGCCATCGA

Product: agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTASPSFLGLPDRLDGRTPRAVIFGAGHGSTYPGKDSSGHALAADAIRAASQDDASLVEHWDFDLGGPLFNGKPASCVDA
GDISTILHDNAENRVRIEAKTREVLALPAVPILLGGDDSVVIPFLAGFADHGPIWVLQIDAHIDWRDEVLGERHGYSSPM
RRASEMPHVAGIVQVGLRSVGSARLAEIEAARHYGSRFVTAREVHAQGVDAALRHIPEGARVVATFDCDSLDPGIMPGVA
ARTPGGLTYTQAIDLIAGLGKRARIAGFDLVELYPPADIDGLSALTAARLLVNVIGTIASMTVSQSSSTA

Sequences:

>Translated_310_residues
MTASPSFLGLPDRLDGRTPRAVIFGAGHGSTYPGKDSSGHALAADAIRAASQDDASLVEHWDFDLGGPLFNGKPASCVDA
GDISTILHDNAENRVRIEAKTREVLALPAVPILLGGDDSVVIPFLAGFADHGPIWVLQIDAHIDWRDEVLGERHGYSSPM
RRASEMPHVAGIVQVGLRSVGSARLAEIEAARHYGSRFVTAREVHAQGVDAALRHIPEGARVVATFDCDSLDPGIMPGVA
ARTPGGLTYTQAIDLIAGLGKRARIAGFDLVELYPPADIDGLSALTAARLLVNVIGTIASMTVSQSSSTA
>Mature_309_residues
TASPSFLGLPDRLDGRTPRAVIFGAGHGSTYPGKDSSGHALAADAIRAASQDDASLVEHWDFDLGGPLFNGKPASCVDAG
DISTILHDNAENRVRIEAKTREVLALPAVPILLGGDDSVVIPFLAGFADHGPIWVLQIDAHIDWRDEVLGERHGYSSPMR
RASEMPHVAGIVQVGLRSVGSARLAEIEAARHYGSRFVTAREVHAQGVDAALRHIPEGARVVATFDCDSLDPGIMPGVAA
RTPGGLTYTQAIDLIAGLGKRARIAGFDLVELYPPADIDGLSALTAARLLVNVIGTIASMTVSQSSSTA

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=231, Percent_Identity=31.6017316017316, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1789306, Length=244, Percent_Identity=31.5573770491803, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32548; Mature: 32417

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPSFLGLPDRLDGRTPRAVIFGAGHGSTYPGKDSSGHALAADAIRAASQDDASLVEH
CCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHH
WDFDLGGPLFNGKPASCVDAGDISTILHDNAENRVRIEAKTREVLALPAVPILLGGDDSV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCEEEEECCEEEEECCCCCE
VIPFLAGFADHGPIWVLQIDAHIDWRDEVLGERHGYSSPMRRASEMPHVAGIVQVGLRSV
EHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHC
GSARLAEIEAARHYGSRFVTAREVHAQGVDAALRHIPEGARVVATFDCDSLDPGIMPGVA
CCHHHHHHHHHHHHHHHCEEHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
ARTPGGLTYTQAIDLIAGLGKRARIAGFDLVELYPPADIDGLSALTAARLLVNVIGTIAS
CCCCCCCHHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MTVSQSSSTA
HHCCCCCCCC
>Mature Secondary Structure 
TASPSFLGLPDRLDGRTPRAVIFGAGHGSTYPGKDSSGHALAADAIRAASQDDASLVEH
CCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHH
WDFDLGGPLFNGKPASCVDAGDISTILHDNAENRVRIEAKTREVLALPAVPILLGGDDSV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCEEEEECCEEEEECCCCCE
VIPFLAGFADHGPIWVLQIDAHIDWRDEVLGERHGYSSPMRRASEMPHVAGIVQVGLRSV
EHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHC
GSARLAEIEAARHYGSRFVTAREVHAQGVDAALRHIPEGARVVATFDCDSLDPGIMPGVA
CCHHHHHHHHHHHHHHHCEEHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
ARTPGGLTYTQAIDLIAGLGKRARIAGFDLVELYPPADIDGLSALTAARLLVNVIGTIAS
CCCCCCCHHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MTVSQSSSTA
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2377617 [H]