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The map label for this gene is gcl [H]

Identifier: 13470522

GI number: 13470522

Start: 177553

End: 179334

Strand: Direct

Name: gcl [H]

Synonym: mlr0251

Alternate gene names: 13470522

Gene position: 177553-179334 (Clockwise)

Preceding gene: 13470519

Following gene: 13470523

Centisome position: 2.52

GC content: 63.52

Gene sequence:

>1782_bases
ATGGCCAGGATGCGCGCTGTCGATGCGGCGGTTCTCGTTCTCGAAAAGGAAGGTATCGCCTGCGCCTTTGGCGTGCCGGG
TGCGGCGATCAATCCGCTCTATTCGGCGCTGAAGGCGAGGGGCACAATCCGCCATGTCCTTGCCCGCCACGTCGAGGGCG
CCTCGCATATGGCCGAAGGCTTCACCCGCGCCAAGGCGGGCAATATCGGGCTCTGCATCGGCACGTCCGGGCCGGCCGGC
ACCGATATGATCACCGGTCTTTATTCGGCCGCCGCCGATTCCATTCCGATCCTGTGCATCACCGGCCAGGCGCCGCGGGC
ACGGCTGAACAAGGAGGATTTCCAGGCTGTCGACATCGCCGCCATCGCCGCACCGGTCGCCAAATGGGCGGTCACCGTGA
TGGAGCCTTATCTCGTGCCGATGGCGCTGCAGAAGGCGTTTCACCTCATGCGCTCATCGCGGCCCGGCCCGGTGCTGATC
GACCTGCCGGTAGACGTCCAACTGGCCGAGATCGAGTTCGATATTGATGCCTATGAACCGATGGCGCCGTTCAAGCCGGC
GATGACGCGCGTCCAGGCCGAAAAGGCGTTGGCGATGCTCAACGCGGCGGAAAAGCCGTTGATCGTCGCCGGCGGCGGCA
TCATCAATGCCGATGCATCCGACTTGCTGATCGAATTCGCGGAAATATCAGGCGTTCCGGTTATTCCGACGCTGATGGGC
TGGGGCGCGATCCCCGACGACCACCGGCTGATGGCCGGCATGTGCGGTCTGCAGACCTCGCATCGCTACGGCAACGCCAC
GATGCTGGAAGCCGATTTCGTCTTCGGCATCGGCAATCGCTGGGCCAACCGTCACACCGGTTCTGTGGACGTCTACACCA
AGGGCAAGAAGTTCATCCATGTCGACATCGAGCCCACGCAGATCGGCCGTGTCTTCGCGCCGGATCTCGGCGTCGTCTCG
GATGCGGGGGCGGCACTGAAAATGCTGCTCGACGTCGCCACCGAGTGGAAGACGGCCGGAAAGCTGCGCGACTGGTCGGG
CTGGGCGAAGGAATGCCAGGCCCGCAAGAAGACGATGAAGCGCAAGACACATTTCGACCAGGTGCCGCTGAAGCCGCAGC
GCGTCTATGAGGAGATGAACAAGGCGTTCGGCCGCGATGTCACCTATGTCACCACGATCGGCCTGTCGCAGATCGCCGGC
GCGCAGTTCCTGCACGTCTACAAGCCGCGCAACTGGATTAATTGCGGCCAGGCCGGCCCGCTCGGCTGGACCTTGCCGGC
AGCCCTTGGCGTGCGCGCCGCCGATCCCGAGCGCACCATCGTGGCGCTGTCGGGCGACTATGATTTCCAGTTCATGATCG
AGGAACTGGCGGTCGGCGCGCAGCACAAGCTGCCCTACATCCATGTCGTCGTGAACAATGCCTATCTCGGCCTGATCCGC
CAGGCGCAGCGCGGCTTCTCGATGGATTATGAAGTCAGCCTCGCCTTCGAGAACATCAACCGATCGGGCGATCCCGAGGC
CGGCTATGGCGTCGACCATGTCGCCGTCGCCGAAGCGATGGGCTGCAAGGCGGTCCGGGTGCGCAAGCCCGAGGAGTTCG
CCGGCGCCTTCAGAGAGGCGCAGCGGCTGATGAAGGAGCACCGGGTTCCCGTTGTGCTCGAATTCATCCTCGAGCGCGTC
ACCAACATCTCCATGGGTACCGAGATCGACAAGATCACCGAATTCGAGGACCTCGCCGAAAGCCATGAGGACGCGCCGAC
CGCGATCGTGATGTTGGATTAG

Upstream 100 bases:

>100_bases
TTTCTAGAAATGAAAAAATATTCTGAAAAAATTGAAAAATAGCCGGCTTGCTGACATTCTCGGCCATCCAACAATAACAA
CCCAAGTGGAGGGCCGGAAA

Downstream 100 bases:

>100_bases
GAGAAAAAGAATGCCGCGTTTTTCCGCCAATCTGTCCATGCTCTTTGCCGAGCATGATTTCCTCGACCGCTTTGATGCAG
CCGCCCGCGCGGGGTTCAAG

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MARMRAVDAAVLVLEKEGIACAFGVPGAAINPLYSALKARGTIRHVLARHVEGASHMAEGFTRAKAGNIGLCIGTSGPAG
TDMITGLYSAAADSIPILCITGQAPRARLNKEDFQAVDIAAIAAPVAKWAVTVMEPYLVPMALQKAFHLMRSSRPGPVLI
DLPVDVQLAEIEFDIDAYEPMAPFKPAMTRVQAEKALAMLNAAEKPLIVAGGGIINADASDLLIEFAEISGVPVIPTLMG
WGAIPDDHRLMAGMCGLQTSHRYGNATMLEADFVFGIGNRWANRHTGSVDVYTKGKKFIHVDIEPTQIGRVFAPDLGVVS
DAGAALKMLLDVATEWKTAGKLRDWSGWAKECQARKKTMKRKTHFDQVPLKPQRVYEEMNKAFGRDVTYVTTIGLSQIAG
AQFLHVYKPRNWINCGQAGPLGWTLPAALGVRAADPERTIVALSGDYDFQFMIEELAVGAQHKLPYIHVVVNNAYLGLIR
QAQRGFSMDYEVSLAFENINRSGDPEAGYGVDHVAVAEAMGCKAVRVRKPEEFAGAFREAQRLMKEHRVPVVLEFILERV
TNISMGTEIDKITEFEDLAESHEDAPTAIVMLD

Sequences:

>Translated_593_residues
MARMRAVDAAVLVLEKEGIACAFGVPGAAINPLYSALKARGTIRHVLARHVEGASHMAEGFTRAKAGNIGLCIGTSGPAG
TDMITGLYSAAADSIPILCITGQAPRARLNKEDFQAVDIAAIAAPVAKWAVTVMEPYLVPMALQKAFHLMRSSRPGPVLI
DLPVDVQLAEIEFDIDAYEPMAPFKPAMTRVQAEKALAMLNAAEKPLIVAGGGIINADASDLLIEFAEISGVPVIPTLMG
WGAIPDDHRLMAGMCGLQTSHRYGNATMLEADFVFGIGNRWANRHTGSVDVYTKGKKFIHVDIEPTQIGRVFAPDLGVVS
DAGAALKMLLDVATEWKTAGKLRDWSGWAKECQARKKTMKRKTHFDQVPLKPQRVYEEMNKAFGRDVTYVTTIGLSQIAG
AQFLHVYKPRNWINCGQAGPLGWTLPAALGVRAADPERTIVALSGDYDFQFMIEELAVGAQHKLPYIHVVVNNAYLGLIR
QAQRGFSMDYEVSLAFENINRSGDPEAGYGVDHVAVAEAMGCKAVRVRKPEEFAGAFREAQRLMKEHRVPVVLEFILERV
TNISMGTEIDKITEFEDLAESHEDAPTAIVMLD
>Mature_592_residues
ARMRAVDAAVLVLEKEGIACAFGVPGAAINPLYSALKARGTIRHVLARHVEGASHMAEGFTRAKAGNIGLCIGTSGPAGT
DMITGLYSAAADSIPILCITGQAPRARLNKEDFQAVDIAAIAAPVAKWAVTVMEPYLVPMALQKAFHLMRSSRPGPVLID
LPVDVQLAEIEFDIDAYEPMAPFKPAMTRVQAEKALAMLNAAEKPLIVAGGGIINADASDLLIEFAEISGVPVIPTLMGW
GAIPDDHRLMAGMCGLQTSHRYGNATMLEADFVFGIGNRWANRHTGSVDVYTKGKKFIHVDIEPTQIGRVFAPDLGVVSD
AGAALKMLLDVATEWKTAGKLRDWSGWAKECQARKKTMKRKTHFDQVPLKPQRVYEEMNKAFGRDVTYVTTIGLSQIAGA
QFLHVYKPRNWINCGQAGPLGWTLPAALGVRAADPERTIVALSGDYDFQFMIEELAVGAQHKLPYIHVVVNNAYLGLIRQ
AQRGFSMDYEVSLAFENINRSGDPEAGYGVDHVAVAEAMGCKAVRVRKPEEFAGAFREAQRLMKEHRVPVVLEFILERVT
NISMGTEIDKITEFEDLAESHEDAPTAIVMLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=23.2142857142857, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI21361361, Length=540, Percent_Identity=22.7777777777778, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786717, Length=592, Percent_Identity=72.8040540540541, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=34.1030195381883, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=33.0578512396694, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1787096, Length=531, Percent_Identity=25.8003766478343, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788716, Length=482, Percent_Identity=27.8008298755187, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=496, Percent_Identity=25.2016129032258, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=496, Percent_Identity=25.2016129032258, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=604, Percent_Identity=29.9668874172185, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=568, Percent_Identity=23.943661971831, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19922626, Length=561, Percent_Identity=24.5989304812834, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64461; Mature: 64330

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMRAVDAAVLVLEKEGIACAFGVPGAAINPLYSALKARGTIRHVLARHVEGASHMAEG
CCCCHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FTRAKAGNIGLCIGTSGPAGTDMITGLYSAAADSIPILCITGQAPRARLNKEDFQAVDIA
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
AIAAPVAKWAVTVMEPYLVPMALQKAFHLMRSSRPGPVLIDLPVDVQLAEIEFDIDAYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
MAPFKPAMTRVQAEKALAMLNAAEKPLIVAGGGIINADASDLLIEFAEISGVPVIPTLMG
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGAIPDDHRLMAGMCGLQTSHRYGNATMLEADFVFGIGNRWANRHTGSVDVYTKGKKFIH
CCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFAPDLGVVSDAGAALKMLLDVATEWKTAGKLRDWSGWAKECQARKKTMK
EECCHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RKTHFDQVPLKPQRVYEEMNKAFGRDVTYVTTIGLSQIAGAQFLHVYKPRNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCEEEEECCCCCCCCCCCCC
LGWTLPAALGVRAADPERTIVALSGDYDFQFMIEELAVGAQHKLPYIHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QAQRGFSMDYEVSLAFENINRSGDPEAGYGVDHVAVAEAMGCKAVRVRKPEEFAGAFREA
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHH
QRLMKEHRVPVVLEFILERVTNISMGTEIDKITEFEDLAESHEDAPTAIVMLD
HHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
ARMRAVDAAVLVLEKEGIACAFGVPGAAINPLYSALKARGTIRHVLARHVEGASHMAEG
CCCHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FTRAKAGNIGLCIGTSGPAGTDMITGLYSAAADSIPILCITGQAPRARLNKEDFQAVDIA
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
AIAAPVAKWAVTVMEPYLVPMALQKAFHLMRSSRPGPVLIDLPVDVQLAEIEFDIDAYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
MAPFKPAMTRVQAEKALAMLNAAEKPLIVAGGGIINADASDLLIEFAEISGVPVIPTLMG
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGAIPDDHRLMAGMCGLQTSHRYGNATMLEADFVFGIGNRWANRHTGSVDVYTKGKKFIH
CCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFAPDLGVVSDAGAALKMLLDVATEWKTAGKLRDWSGWAKECQARKKTMK
EECCHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RKTHFDQVPLKPQRVYEEMNKAFGRDVTYVTTIGLSQIAGAQFLHVYKPRNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCEEEEECCCCCCCCCCCCC
LGWTLPAALGVRAADPERTIVALSGDYDFQFMIEELAVGAQHKLPYIHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QAQRGFSMDYEVSLAFENINRSGDPEAGYGVDHVAVAEAMGCKAVRVRKPEEFAGAFREA
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHH
QRLMKEHRVPVVLEFILERVTNISMGTEIDKITEFEDLAESHEDAPTAIVMLD
HHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]