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The map label for this gene is sgbE [H]

Identifier: 161723310

GI number: 161723310

Start: 1437591

End: 1438286

Strand: Reverse

Name: sgbE [H]

Synonym: PM1244

Alternate gene names: 161723310

Gene position: 1438286-1437591 (Counterclockwise)

Preceding gene: 15603110

Following gene: 15603107

Centisome position: 63.71

GC content: 43.68

Gene sequence:

>696_bases
ATGTTAGAAGAATTGAAACAAAAAGTGTTTGAAGCCAATTTGGCATTACCCAAATACAAGTTAGTGACCTTTACTTGGGG
TAACGTGAGTGGCATCGACAGAGAAAAAAATTTGGTTGTGATTAAGCCCTCCGGCGTAGAGTATGACACCATGACAGTGG
AGGATATGGTAGTAGTTGATCTCTTTACAGGGCAAGTGGTGGAAGGCAACAAAAAACCTTCCTCAGATACCGCGACCCAT
TTAGAACTTTATCGTCAATTTCCAAGCTTAGGCGGGATTGTTCATACTCACTCACGCCACGCTACGATTTGGGCGCAAGC
CGGTGAAGATTTGATTGCGGCGGGGACGACTCATGCTGATTATTTCTATGGTTCAATTCCTTGTACACGTAAAATGACCC
CTGCCGAAATTCAAGGCGAATATGAGCTTGAAACCGGCAAAGTGATTGTGGAAACATTCCGTGTGAGAGGAATCGATCCG
AAAGATGTACCAGCAGTATTAGTGCACTCCCATGGTCCTTTTGCTTGGGGTACCGATCCTGATAATGCCGTACATAATGC
AGTGGTTCTGGAAGAGATTGGCTATATGAATTTATTTAGTCGTCAACTGCGTCCAAATTTAGCGTCGATGCAGCAAGAAT
TGCTAGATAAACACTACTTACGTAAACATGGGAAAAACGCGTATTACGGGCAATAA

Upstream 100 bases:

>100_bases
TTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCCATTGCTGAAATTATCAACGCACGTCGTTGGATCGAACAAAAA
ATGAAAGAAGGTGGTTTCCA

Downstream 100 bases:

>100_bases
AACGCACTAAGCAAAAGAGCTAAAGGAGGTACTTTAGCTCTTTATCCTTAAAAGGTGATTAGTCGAGAACGGTAGCAAGG
ACAGACGCGATTCCGTCTTG

Product: L-ribulose-5-phosphate 4-epimerase

Products: D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLEELKQKVFEANLALPKYKLVTFTWGNVSGIDREKNLVVIKPSGVEYDTMTVEDMVVVDLFTGQVVEGNKKPSSDTATH
LELYRQFPSLGGIVHTHSRHATIWAQAGEDLIAAGTTHADYFYGSIPCTRKMTPAEIQGEYELETGKVIVETFRVRGIDP
KDVPAVLVHSHGPFAWGTDPDNAVHNAVVLEEIGYMNLFSRQLRPNLASMQQELLDKHYLRKHGKNAYYGQ

Sequences:

>Translated_231_residues
MLEELKQKVFEANLALPKYKLVTFTWGNVSGIDREKNLVVIKPSGVEYDTMTVEDMVVVDLFTGQVVEGNKKPSSDTATH
LELYRQFPSLGGIVHTHSRHATIWAQAGEDLIAAGTTHADYFYGSIPCTRKMTPAEIQGEYELETGKVIVETFRVRGIDP
KDVPAVLVHSHGPFAWGTDPDNAVHNAVVLEEIGYMNLFSRQLRPNLASMQQELLDKHYLRKHGKNAYYGQ
>Mature_231_residues
MLEELKQKVFEANLALPKYKLVTFTWGNVSGIDREKNLVVIKPSGVEYDTMTVEDMVVVDLFTGQVVEGNKKPSSDTATH
LELYRQFPSLGGIVHTHSRHATIWAQAGEDLIAAGTTHADYFYGSIPCTRKMTPAEIQGEYELETGKVIVETFRVRGIDP
KDVPAVLVHSHGPFAWGTDPDNAVHNAVVLEEIGYMNLFSRQLRPNLASMQQELLDKHYLRKHGKNAYYGQ

Specific function: Probable pentulose-5-phosphate-4-epimerase [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=71.8614718614719, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=71.4285714285714, Blast_Score=354, Evalue=3e-99,
Organism=Escherichia coli, GI1790642, Length=230, Percent_Identity=63.4782608695652, Blast_Score=310, Evalue=4e-86,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR004661 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 5.1.3.4

Molecular weight: Translated: 25901; Mature: 25901

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEELKQKVFEANLALPKYKLVTFTWGNVSGIDREKNLVVIKPSGVEYDTMTVEDMVVVD
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCEEEHHHEEEEE
LFTGQVVEGNKKPSSDTATHLELYRQFPSLGGIVHTHSRHATIWAQAGEDLIAAGTTHAD
EECCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEECCCCCC
YFYGSIPCTRKMTPAEIQGEYELETGKVIVETFRVRGIDPKDVPAVLVHSHGPFAWGTDP
EEECCCCCCCCCCCHHCCCCEEECCCCEEEEEHHHCCCCCCCCCEEEEECCCCCCCCCCC
DNAVHNAVVLEEIGYMNLFSRQLRPNLASMQQELLDKHYLRKHGKNAYYGQ
CHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLEELKQKVFEANLALPKYKLVTFTWGNVSGIDREKNLVVIKPSGVEYDTMTVEDMVVVD
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCEEEHHHEEEEE
LFTGQVVEGNKKPSSDTATHLELYRQFPSLGGIVHTHSRHATIWAQAGEDLIAAGTTHAD
EECCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEECCCCCC
YFYGSIPCTRKMTPAEIQGEYELETGKVIVETFRVRGIDPKDVPAVLVHSHGPFAWGTDP
EEECCCCCCCCCCCHHCCCCEEECCCCEEEEEHHHCCCCCCCCCEEEEECCCCCCCCCCC
DNAVHNAVVLEEIGYMNLFSRQLRPNLASMQQELLDKHYLRKHGKNAYYGQ
CHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-ribulose 5-phosphate

Specific reaction: L-ribulose 5-phosphate = D-xylulose 5-phosphate

General reaction: Epimerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]