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The map label for this gene is holB [H]

Identifier: 15603539

GI number: 15603539

Start: 1881055

End: 1882038

Strand: Direct

Name: holB [H]

Synonym: PM1674

Alternate gene names: 15603539

Gene position: 1881055-1882038 (Clockwise)

Preceding gene: 161723306

Following gene: 15603540

Centisome position: 83.33

GC content: 42.38

Gene sequence:

>984_bases
ATGATGACATTGTATCCTTGGCTGTTGCCTTACTATCAACAACGTATTGATGCCTTCCAACAAGGACATGGGCATCATGC
GCTCTTGTTTCAAGCCGAGCAAGGCTTAGGCACTGAACAGCTATTATTTGCCTTAGGTCATTGGCTCATTTGCCAGCAAC
CGCAAAACCAACAACCTTGCCAACAATGTCATCATTGTCATTTGTTTCAAGCCCAAACCCATCCTGATATCTATACCTTG
ACCCCGATTGAAAATAAAGATATTGGTGTGGATCAAGTGCGTGAAGTGAATGAGAAAATTAATCAACATGCCCAACAAGG
GGGCAATAAGATCATCTATGTGCTGGGTGTTTCGCGTTTAACCGAAGCCGCTGCCAATGCGATGTTAAAAACCCTTGAAG
AACCACGTCCAAATACTTATTTTCTGCTATACACAGAAGCATCCGACTCAGTGATGCCGACGATTTATAGTCGCTGCCAA
ACGCAAAAATTGGCGCTCCCAGCTGAAACCAGTGCGATCGCTTGGTTACAGCAACAAACGACACAAGAAATTGCAGCAAT
CCAGACCGCACTTCGTATTAGCTATGGGCGACCATTGCATGCCTTAACGGTACTACAACAAGATTTATTGGAAAAACGTC
GTGAGTTTTTGCGTCAATTCTGGCTATTTTATCGGAAACGTTCTCCGTTAGAACTGTTACCTTTTTTTGATAAAGCCATT
CTCCTCCACCAACTTGACTGGTTACTCGCTTTTTTAAGTGATGCTCTCAAAGCAAAATTACAGATTAAAAGCGATTGGTT
ATGTCAGGATCTCGCTGCTGGCGTACTGCAATTAAGTCAACAACAAAGCGCACAGGCATTACTTCATGCCACACAAATCA
TACAGAAAGTACGTACCGATTTAACGCAAATTAATGCCGTCAACCAAGAACTCATCTTATTAGACGGTCTCACTCAATTA
ATTACCGATGTATTTAAAGGATAA

Upstream 100 bases:

>100_bases
AACCCGAAAGCCGTGACCATTGATGCCTCCCAGACGATGAGCAAAGTGGCAGAAGATGTGGAAAGCGCGATAGAGACTTG
GTTGACTACACGTTAAACAG

Downstream 100 bases:

>100_bases
AATATGTTTATTGTCGATTCCCACTGTCATCTTGATGCACTGGATTATGAAAACTTACACAGCAGTGTTGATGACGTGGT
TAACAAAGCCATGGAAAGAG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MMTLYPWLLPYYQQRIDAFQQGHGHHALLFQAEQGLGTEQLLFALGHWLICQQPQNQQPCQQCHHCHLFQAQTHPDIYTL
TPIENKDIGVDQVREVNEKINQHAQQGGNKIIYVLGVSRLTEAAANAMLKTLEEPRPNTYFLLYTEASDSVMPTIYSRCQ
TQKLALPAETSAIAWLQQQTTQEIAAIQTALRISYGRPLHALTVLQQDLLEKRREFLRQFWLFYRKRSPLELLPFFDKAI
LLHQLDWLLAFLSDALKAKLQIKSDWLCQDLAAGVLQLSQQQSAQALLHATQIIQKVRTDLTQINAVNQELILLDGLTQL
ITDVFKG

Sequences:

>Translated_327_residues
MMTLYPWLLPYYQQRIDAFQQGHGHHALLFQAEQGLGTEQLLFALGHWLICQQPQNQQPCQQCHHCHLFQAQTHPDIYTL
TPIENKDIGVDQVREVNEKINQHAQQGGNKIIYVLGVSRLTEAAANAMLKTLEEPRPNTYFLLYTEASDSVMPTIYSRCQ
TQKLALPAETSAIAWLQQQTTQEIAAIQTALRISYGRPLHALTVLQQDLLEKRREFLRQFWLFYRKRSPLELLPFFDKAI
LLHQLDWLLAFLSDALKAKLQIKSDWLCQDLAAGVLQLSQQQSAQALLHATQIIQKVRTDLTQINAVNQELILLDGLTQL
ITDVFKG
>Mature_327_residues
MMTLYPWLLPYYQQRIDAFQQGHGHHALLFQAEQGLGTEQLLFALGHWLICQQPQNQQPCQQCHHCHLFQAQTHPDIYTL
TPIENKDIGVDQVREVNEKINQHAQQGGNKIIYVLGVSRLTEAAANAMLKTLEEPRPNTYFLLYTEASDSVMPTIYSRCQ
TQKLALPAETSAIAWLQQQTTQEIAAIQTALRISYGRPLHALTVLQQDLLEKRREFLRQFWLFYRKRSPLELLPFFDKAI
LLHQLDWLLAFLSDALKAKLQIKSDWLCQDLAAGVLQLSQQQSAQALLHATQIIQKVRTDLTQINAVNQELILLDGLTQL
ITDVFKG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=323, Percent_Identity=38.0804953560372, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1786676, Length=138, Percent_Identity=28.9855072463768, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37489; Mature: 37489

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLYPWLLPYYQQRIDAFQQGHGHHALLFQAEQGLGTEQLLFALGHWLICQQPQNQQPC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHEECCCCCCCHH
QQCHHCHLFQAQTHPDIYTLTPIENKDIGVDQVREVNEKINQHAQQGGNKIIYVLGVSRL
HHHHHCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHH
TEAAANAMLKTLEEPRPNTYFLLYTEASDSVMPTIYSRCQTQKLALPAETSAIAWLQQQT
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TQEIAAIQTALRISYGRPLHALTVLQQDLLEKRREFLRQFWLFYRKRSPLELLPFFDKAI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LLHQLDWLLAFLSDALKAKLQIKSDWLCQDLAAGVLQLSQQQSAQALLHATQIIQKVRTD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQINAVNQELILLDGLTQLITDVFKG
HHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTLYPWLLPYYQQRIDAFQQGHGHHALLFQAEQGLGTEQLLFALGHWLICQQPQNQQPC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHEECCCCCCCHH
QQCHHCHLFQAQTHPDIYTLTPIENKDIGVDQVREVNEKINQHAQQGGNKIIYVLGVSRL
HHHHHCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHH
TEAAANAMLKTLEEPRPNTYFLLYTEASDSVMPTIYSRCQTQKLALPAETSAIAWLQQQT
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TQEIAAIQTALRISYGRPLHALTVLQQDLLEKRREFLRQFWLFYRKRSPLELLPFFDKAI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LLHQLDWLLAFLSDALKAKLQIKSDWLCQDLAAGVLQLSQQQSAQALLHATQIIQKVRTD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQINAVNQELILLDGLTQLITDVFKG
HHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]