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The map label for this gene is def

Identifier: 15603424

GI number: 15603424

Start: 1767075

End: 1767587

Strand: Direct

Name: def

Synonym: PM1559

Alternate gene names: 15603424

Gene position: 1767075-1767587 (Clockwise)

Preceding gene: 15603422

Following gene: 15603425

Centisome position: 78.28

GC content: 37.82

Gene sequence:

>513_bases
ATGGCGCGATTAAATGTACTTGTTTATCCAGATGAACGTTTGAAAATCATTGCGAAACCTGTTGTAGAGGTAAATGACGA
AATTCGTGAAATTGTGGATAATATGTTTGAAACAATGTACCTTGAAGAAGGGATTGGATTAGCAGCAACACAGGTTAATA
TTCATCAACGTATTATTACGATTGATGTGGAAGGTACGAAAGAAAACCAGTATGTGTTAATTAATCCTGAAATTATTGAT
AGCTGTGGTGAAACAGGGATTGAGGAAGGCTGTTTATCTTTACCTGGATTCCGCGGTTTTGTGCCACGTAAAGAAAAAGT
CACGATTAAAGCGTTGGATCGTCATGGCGAAGAATATACGTTGAGCGCCGAGGGGCTATTGGCGATCTGTATTCAACATG
AAATCGATCACTTAAATGGCATTGTGTTTGCTGATTATTTATCACCGTTGAAACGTCAACGTATGAAAGAAAAATTGCTG
AAACTCCAAAAGCAATTAGCAAGACAGAAGTAA

Upstream 100 bases:

>100_bases
CATATTTGATATTTTTAGTTTGGGGAAAGTTTTTGTAAAAAATCAAAAAAAGAGTAGAATAATTAAAATTTTTTTATTGC
ATTTTACACAAAGAGAAATT

Downstream 100 bases:

>100_bases
TTATTGATACCCACGTCAGTTAGCGTGGGTTGTTTTATTCATAACGTAGAACATTATTTTATATGACATCACTCAAAATT
ATCTTTGCGGGTACGCCAGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MARLNVLVYPDERLKIIAKPVVEVNDEIREIVDNMFETMYLEEGIGLAATQVNIHQRIITIDVEGTKENQYVLINPEIID
SCGETGIEEGCLSLPGFRGFVPRKEKVTIKALDRHGEEYTLSAEGLLAICIQHEIDHLNGIVFADYLSPLKRQRMKEKLL
KLQKQLARQK

Sequences:

>Translated_170_residues
MARLNVLVYPDERLKIIAKPVVEVNDEIREIVDNMFETMYLEEGIGLAATQVNIHQRIITIDVEGTKENQYVLINPEIID
SCGETGIEEGCLSLPGFRGFVPRKEKVTIKALDRHGEEYTLSAEGLLAICIQHEIDHLNGIVFADYLSPLKRQRMKEKLL
KLQKQLARQK
>Mature_169_residues
ARLNVLVYPDERLKIIAKPVVEVNDEIREIVDNMFETMYLEEGIGLAATQVNIHQRIITIDVEGTKENQYVLINPEIIDS
CGETGIEEGCLSLPGFRGFVPRKEKVTIKALDRHGEEYTLSAEGLLAICIQHEIDHLNGIVFADYLSPLKRQRMKEKLLK
LQKQLARQK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=158, Percent_Identity=29.1139240506329, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=64.8809523809524, Blast_Score=221, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24645728, Length=138, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24645726, Length=141, Percent_Identity=29.0780141843972, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PASMU (P57948)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246498.1
- ProteinModelPortal:   P57948
- SMR:   P57948
- GeneID:   1244906
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1559
- NMPDR:   fig|272843.1.peg.1560
- HOGENOM:   HBG665227
- OMA:   GAKHEER
- ProtClustDB:   PRK00150
- BioCyc:   PMUL272843:PM1559-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19386; Mature: 19255

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLNVLVYPDERLKIIAKPVVEVNDEIREIVDNMFETMYLEEGIGLAATQVNIHQRIIT
CCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEE
IDVEGTKENQYVLINPEIIDSCGETGIEEGCLSLPGFRGFVPRKEKVTIKALDRHGEEYT
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCEEE
LSAEGLLAICIQHEIDHLNGIVFADYLSPLKRQRMKEKLLKLQKQLARQK
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARLNVLVYPDERLKIIAKPVVEVNDEIREIVDNMFETMYLEEGIGLAATQVNIHQRIIT
CEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEE
IDVEGTKENQYVLINPEIIDSCGETGIEEGCLSLPGFRGFVPRKEKVTIKALDRHGEEYT
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCEEE
LSAEGLLAICIQHEIDHLNGIVFADYLSPLKRQRMKEKLLKLQKQLARQK
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100