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The map label for this gene is fmt

Identifier: 15603425

GI number: 15603425

Start: 1767650

End: 1768603

Strand: Direct

Name: fmt

Synonym: PM1560

Alternate gene names: 15603425

Gene position: 1767650-1768603 (Clockwise)

Preceding gene: 15603424

Following gene: 15603426

Centisome position: 78.3

GC content: 42.66

Gene sequence:

>954_bases
ATGACATCACTCAAAATTATCTTTGCGGGTACGCCAGCGTTTGCTGCCCAGCATTTACAAGCTTTATTGAATTCTCATCA
TCAAGTGGTTGCTGTTTACACGCAACCTGACAAACCTGCTGGGCGAGGCAAAAAGTTACAAGCCAGTCCAGTGAAACAAT
TAGCAGAGCAGCATAATATTCCCGTTTATCAACCGAAATCCTTGCGTAAAGTAGAAGCACAAGAAGAAATGCGAGCCATT
GATGCAGATGTGATGGTTGTTGTGGCTTATGGTTTGATTTTACCGCAGACCGTATTAGCTATGCCTCGTTTAGGTTGTTT
GAATGTGCATGGATCGCTGTTACCGCGTTGGCGTGGGGCGGCGCCGATTCAACGTGCCATTTGGGCTGGGGATAAACAAA
CGGGTATCACTATTATGCAAATGGATGAAGGATTGGATACAGGTGATATGTTGTATAAAGTGTATTGTGATATTGCGCAG
GATGAAACATCCACTAGTCTGTATGCTAAATTAATGGAAATTGCCCCCCCCGCCTTATTACATGTCCTCGATGGATTAGA
TAAAGGAAAATTTGTATCTGAGAAACAAGATGATATAGAATCATGTTATGCAGAAAAATTGTCTAAAGAAGAAGCAGAAT
TAAATTGGCATGTCTCTGCAGAGCAGTTAGAACGTCATATCCGTGCGTTTAATCCTTGGCCAGTGAGTTATTTTTCTACA
CGCGATCTCCAAGGCAATACCCAGGTGATAAAAGTCTATAAAGCCGCTGTTTTACCTCATGTTGATAAAGTGGCTGGGAC
GATTTTAAGTGCGGATAAAAATGGTATTCAAATTGCGACTGCAGAAGGTGTATTAAATTTGTTGCAATTACAACCCGCGG
GGAAAAAGCCAATGGATGCGCGGGACTTGCTGAATGGGCGTGCAGAATGGTTTCCTGTCGGTAAGGTTTTATGA

Upstream 100 bases:

>100_bases
TGCTGAAACTCCAAAAGCAATTAGCAAGACAGAAGTAATTATTGATACCCACGTCAGTTAGCGTGGGTTGTTTTATTCAT
AACGTAGAACATTATTTTAT

Downstream 100 bases:

>100_bases
TGCAGAAATTCGTAAAAAACGTGAAAAAGCAAACTGTACTTCTTTCAACAAGAGCCATTGCTGCACAAATCATTTTACAA
GTTCTGGATCAAGGTAAATC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTSLKIIFAGTPAFAAQHLQALLNSHHQVVAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKVEAQEEMRAI
DADVMVVVAYGLILPQTVLAMPRLGCLNVHGSLLPRWRGAAPIQRAIWAGDKQTGITIMQMDEGLDTGDMLYKVYCDIAQ
DETSTSLYAKLMEIAPPALLHVLDGLDKGKFVSEKQDDIESCYAEKLSKEEAELNWHVSAEQLERHIRAFNPWPVSYFST
RDLQGNTQVIKVYKAAVLPHVDKVAGTILSADKNGIQIATAEGVLNLLQLQPAGKKPMDARDLLNGRAEWFPVGKVL

Sequences:

>Translated_317_residues
MTSLKIIFAGTPAFAAQHLQALLNSHHQVVAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKVEAQEEMRAI
DADVMVVVAYGLILPQTVLAMPRLGCLNVHGSLLPRWRGAAPIQRAIWAGDKQTGITIMQMDEGLDTGDMLYKVYCDIAQ
DETSTSLYAKLMEIAPPALLHVLDGLDKGKFVSEKQDDIESCYAEKLSKEEAELNWHVSAEQLERHIRAFNPWPVSYFST
RDLQGNTQVIKVYKAAVLPHVDKVAGTILSADKNGIQIATAEGVLNLLQLQPAGKKPMDARDLLNGRAEWFPVGKVL
>Mature_316_residues
TSLKIIFAGTPAFAAQHLQALLNSHHQVVAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKVEAQEEMRAID
ADVMVVVAYGLILPQTVLAMPRLGCLNVHGSLLPRWRGAAPIQRAIWAGDKQTGITIMQMDEGLDTGDMLYKVYCDIAQD
ETSTSLYAKLMEIAPPALLHVLDGLDKGKFVSEKQDDIESCYAEKLSKEEAELNWHVSAEQLERHIRAFNPWPVSYFSTR
DLQGNTQVIKVYKAAVLPHVDKVAGTILSADKNGIQIATAEGVLNLLQLQPAGKKPMDARDLLNGRAEWFPVGKVL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=261, Percent_Identity=29.5019157088123, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI21614513, Length=240, Percent_Identity=30, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=31.063829787234, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1789683, Length=316, Percent_Identity=62.6582278481013, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788589, Length=287, Percent_Identity=27.8745644599303, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI133930964, Length=262, Percent_Identity=26.3358778625954, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=29.5081967213115, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=30.8370044052863, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585660, Length=260, Percent_Identity=26.9230769230769, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PASMU (P57949)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246499.1
- ProteinModelPortal:   P57949
- SMR:   P57949
- GeneID:   1244907
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1560
- NMPDR:   fig|272843.1.peg.1561
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   PMUL272843:PM1560-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34977; Mature: 34846

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLKIIFAGTPAFAAQHLQALLNSHHQVVAVYTQPDKPAGRGKKLQASPVKQLAEQHNI
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
PVYQPKSLRKVEAQEEMRAIDADVMVVVAYGLILPQTVLAMPRLGCLNVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
APIQRAIWAGDKQTGITIMQMDEGLDTGDMLYKVYCDIAQDETSTSLYAKLMEIAPPALL
CHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
HVLDGLDKGKFVSEKQDDIESCYAEKLSKEEAELNWHVSAEQLERHIRAFNPWPVSYFST
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCCCEEC
RDLQGNTQVIKVYKAAVLPHVDKVAGTILSADKNGIQIATAEGVLNLLQLQPAGKKPMDA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCH
RDLLNGRAEWFPVGKVL
HHHHCCCCCEEECCCCC
>Mature Secondary Structure 
TSLKIIFAGTPAFAAQHLQALLNSHHQVVAVYTQPDKPAGRGKKLQASPVKQLAEQHNI
CCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
PVYQPKSLRKVEAQEEMRAIDADVMVVVAYGLILPQTVLAMPRLGCLNVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
APIQRAIWAGDKQTGITIMQMDEGLDTGDMLYKVYCDIAQDETSTSLYAKLMEIAPPALL
CHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
HVLDGLDKGKFVSEKQDDIESCYAEKLSKEEAELNWHVSAEQLERHIRAFNPWPVSYFST
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCCCCEEC
RDLQGNTQVIKVYKAAVLPHVDKVAGTILSADKNGIQIATAEGVLNLLQLQPAGKKPMDA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCH
RDLLNGRAEWFPVGKVL
HHHHCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100