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The map label for this gene is orfG

Identifier: 15603422

GI number: 15603422

Start: 1759385

End: 1759969

Strand: Direct

Name: orfG

Synonym: PM1557

Alternate gene names: 15603422

Gene position: 1759385-1759969 (Clockwise)

Preceding gene: 15603421

Following gene: 15603424

Centisome position: 77.94

GC content: 41.54

Gene sequence:

>585_bases
ATGCAAAAAATTATGATTTCCGATGCGGCTCAAGCGCATTTTCGCCGTTTGCTTGATCAGCAAGAAGAAGGAACTCACAT
TCGCATTTTTGTGGTTAACCCAGGCACCCCCAATGCTGAATGTGGTGTGTCTTATTGCCCGCCCAATGCAGTAGAAGCAA
CGGATACGGAAATGCAATATGCTTCATTTTCAGCTTTTGTGGATGAAATCAGCTTACCTTTTTTAGATGAGGCAGAAATT
GACTATGTCACTGAAGAATTAGGCGCACAGTTAACATTAAAAGCTCCGAATGCCAAAATGCGTAAAGTGGCTGATGATGC
CCCTTTACTTGAACGAGTTGAATATGTGATTCAGACACAAATTAATCCGCAACTTGCAGGACACGGCGGACGTATTACAT
TAATTGAAATCACTGAAGATGGTTATGCCATTTTACAATTTGGTGGTGGCTGTAATGGTTGTTCTATGGTGGATGTGACA
TTAAAAGATGGCATTGAAAAGCAATTACTCAGTTTATTCCCGGGAGAGTTGCAAGGTGCGAAAGATGTTACAGAGCATCA
ACGTGGTGAACATTCTTACTACTGA

Upstream 100 bases:

>100_bases
TAAGGAAAGATTTATTACTGTAAAAGGTAGAAAAAAAAACGAACTCGGAGTATCATTCTTGACAATCTTACTCAAGTATT
CACATTCTTTAGGATAGTTT

Downstream 100 bases:

>100_bases
TCCTGACTGATATTCTTTGAAGTAAGGCTGGTTTCTTATCAAACCAGCCTTCTTTATTTAGATTTGTTTAATTTTGCGTA
ATCCTGCTTCATCTAAAGCA

Product: putative DNA uptake protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MQKIMISDAAQAHFRRLLDQQEEGTHIRIFVVNPGTPNAECGVSYCPPNAVEATDTEMQYASFSAFVDEISLPFLDEAEI
DYVTEELGAQLTLKAPNAKMRKVADDAPLLERVEYVIQTQINPQLAGHGGRITLIEITEDGYAILQFGGGCNGCSMVDVT
LKDGIEKQLLSLFPGELQGAKDVTEHQRGEHSYY

Sequences:

>Translated_194_residues
MQKIMISDAAQAHFRRLLDQQEEGTHIRIFVVNPGTPNAECGVSYCPPNAVEATDTEMQYASFSAFVDEISLPFLDEAEI
DYVTEELGAQLTLKAPNAKMRKVADDAPLLERVEYVIQTQINPQLAGHGGRITLIEITEDGYAILQFGGGCNGCSMVDVT
LKDGIEKQLLSLFPGELQGAKDVTEHQRGEHSYY
>Mature_194_residues
MQKIMISDAAQAHFRRLLDQQEEGTHIRIFVVNPGTPNAECGVSYCPPNAVEATDTEMQYASFSAFVDEISLPFLDEAEI
DYVTEELGAQLTLKAPNAKMRKVADDAPLLERVEYVIQTQINPQLAGHGGRITLIEITEDGYAILQFGGGCNGCSMVDVT
LKDGIEKQLLSLFPGELQGAKDVTEHQRGEHSYY

Specific function: Involved in iron-sulfur cluster biogenesis. Binds a 4Fe- 4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nfuA family

Homologues:

Organism=Escherichia coli, GI1789819, Length=191, Percent_Identity=78.0104712041885, Blast_Score=325, Evalue=9e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFUA_PASMU (Q9CKP9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246496.1
- ProteinModelPortal:   Q9CKP9
- GeneID:   1244904
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1557
- NMPDR:   fig|272843.1.peg.1558
- HOGENOM:   HBG289644
- OMA:   CQGCSAV
- ProtClustDB:   PRK11190
- BioCyc:   PMUL272843:PM1557-MONOMER
- HAMAP:   MF_01637
- InterPro:   IPR017726
- InterPro:   IPR000361
- InterPro:   IPR001075
- Gene3D:   G3DSA:2.60.300.12
- PANTHER:   PTHR10072
- ProDom:   PD002830
- TIGRFAMs:   TIGR03341

Pfam domain/function: PF01521 Fe-S_biosyn; PF01106 NifU; SSF89360 HesB_yadR_yfhF-like

EC number: NA

Molecular weight: Translated: 21379; Mature: 21379

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIMISDAAQAHFRRLLDQQEEGTHIRIFVVNPGTPNAECGVSYCPPNAVEATDTEMQY
CCEEEECHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHH
ASFSAFVDEISLPFLDEAEIDYVTEELGAQLTLKAPNAKMRKVADDAPLLERVEYVIQTQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHC
INPQLAGHGGRITLIEITEDGYAILQFGGGCNGCSMVDVTLKDGIEKQLLSLFPGELQGA
CCCEECCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHCCCCCCCC
KDVTEHQRGEHSYY
HHHHHHHCCCCCCC
>Mature Secondary Structure
MQKIMISDAAQAHFRRLLDQQEEGTHIRIFVVNPGTPNAECGVSYCPPNAVEATDTEMQY
CCEEEECHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHH
ASFSAFVDEISLPFLDEAEIDYVTEELGAQLTLKAPNAKMRKVADDAPLLERVEYVIQTQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHC
INPQLAGHGGRITLIEITEDGYAILQFGGGCNGCSMVDVTLKDGIEKQLLSLFPGELQGA
CCCEECCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHCCCCCCCC
KDVTEHQRGEHSYY
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100