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The map label for this gene is hpaG_1 [H]

Identifier: 15603393

GI number: 15603393

Start: 1727351

End: 1728025

Strand: Direct

Name: hpaG_1 [H]

Synonym: PM1528

Alternate gene names: 15603393

Gene position: 1727351-1728025 (Clockwise)

Preceding gene: 15603392

Following gene: 15603394

Centisome position: 76.52

GC content: 45.78

Gene sequence:

>675_bases
ATGTCTGTCTCTCTAGCTAAACAGATTACCCAACTCAATACTGCGCATAAACCTACGGTGTTTGTCATTGCGTTGAACTC
AACCCAATTATTGCAACGTTGCCAAGCTCAGTTTGAACAAGCGCCTTATCAAGCGTTACCGAAAACCCCAGTTATCTATG
TGTTGCCTGAAGCGACACATAATCGCAGTGGGGCGAACGTGTCACTCCCTGCGGGGAAAAAACAGCTCAGAATGGAACCG
CACTTGGCGGTGGTGTTTGGTCGAGATACCTCACGAGTTTGTGTGGAACAGGCGATGCACTACGTATCCGGGTATCTGCC
GGTGGCACTGTATTCTTTACCCCATGACAGCTATTACCGTCCTGACATTGAAGGGCGTTGCCAAGAGGGCTTCTGTGTTT
TAGGGCAAGAAGTGTTAAAAAGTGCGGTACAAAATCCGGCAGAATTGACAATCAATATTACGGTTAATGGTTCTGTGAAG
AAAAGCTATGTGCATTTAGCCATGAAACATTCGATTCCTGAGTTGATCAGTTTTTTAAGCCAATTTATTGCTTTTAAAGC
GGGTGATATCTTGTTGACGGGGACAGAAGATATGCCACTTCTTGTGGGGGCTGGCGATCAGGTATCCGTTGAGTTTGCTC
AACTGGGTTGTTTAACCAATACGGTAACTGAATGA

Upstream 100 bases:

>100_bases
TGTTGCCATTTATTGCCTCTGATCTTATTCGTATTGCTGTGATTGCCTGCGTTCCGGGCATTGCGCTGTGGCTCGTCCAT
TTATTTTAAGTGAGGAAATT

Downstream 100 bases:

>100_bases
GGTAAGCAGGATTATGAGTAAATATGCAAAAGTGATGTATCAAAATCAAATCCATAGCGTAGAAGTGCGACCAAATGGCT
TGTTAACCGATCAACAACAA

Product: HpaG

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSVSLAKQITQLNTAHKPTVFVIALNSTQLLQRCQAQFEQAPYQALPKTPVIYVLPEATHNRSGANVSLPAGKKQLRMEP
HLAVVFGRDTSRVCVEQAMHYVSGYLPVALYSLPHDSYYRPDIEGRCQEGFCVLGQEVLKSAVQNPAELTINITVNGSVK
KSYVHLAMKHSIPELISFLSQFIAFKAGDILLTGTEDMPLLVGAGDQVSVEFAQLGCLTNTVTE

Sequences:

>Translated_224_residues
MSVSLAKQITQLNTAHKPTVFVIALNSTQLLQRCQAQFEQAPYQALPKTPVIYVLPEATHNRSGANVSLPAGKKQLRMEP
HLAVVFGRDTSRVCVEQAMHYVSGYLPVALYSLPHDSYYRPDIEGRCQEGFCVLGQEVLKSAVQNPAELTINITVNGSVK
KSYVHLAMKHSIPELISFLSQFIAFKAGDILLTGTEDMPLLVGAGDQVSVEFAQLGCLTNTVTE
>Mature_223_residues
SVSLAKQITQLNTAHKPTVFVIALNSTQLLQRCQAQFEQAPYQALPKTPVIYVLPEATHNRSGANVSLPAGKKQLRMEPH
LAVVFGRDTSRVCVEQAMHYVSGYLPVALYSLPHDSYYRPDIEGRCQEGFCVLGQEVLKSAVQNPAELTINITVNGSVKK
SYVHLAMKHSIPELISFLSQFIAFKAGDILLTGTEDMPLLVGAGDQVSVEFAQLGCLTNTVTE

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI40786394, Length=194, Percent_Identity=29.8969072164948, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI156231349, Length=189, Percent_Identity=31.2169312169312, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI215422413, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI66348062, Length=158, Percent_Identity=29.746835443038, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI13654274, Length=158, Percent_Identity=29.746835443038, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787428, Length=185, Percent_Identity=27.027027027027, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17557057, Length=176, Percent_Identity=27.8409090909091, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571789, Length=179, Percent_Identity=27.9329608938547, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 24516; Mature: 24384

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSLAKQITQLNTAHKPTVFVIALNSTQLLQRCQAQFEQAPYQALPKTPVIYVLPEATH
CCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCC
NRSGANVSLPAGKKQLRMEPHLAVVFGRDTSRVCVEQAMHYVSGYLPVALYSLPHDSYYR
CCCCCEEECCCCCHHEECCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCC
PDIEGRCQEGFCVLGQEVLKSAVQNPAELTINITVNGSVKKSYVHLAMKHSIPELISFLS
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
QFIAFKAGDILLTGTEDMPLLVGAGDQVSVEFAQLGCLTNTVTE
HHHHHCCCCEEEECCCCCEEEEECCCCEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSLAKQITQLNTAHKPTVFVIALNSTQLLQRCQAQFEQAPYQALPKTPVIYVLPEATH
CCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCC
NRSGANVSLPAGKKQLRMEPHLAVVFGRDTSRVCVEQAMHYVSGYLPVALYSLPHDSYYR
CCCCCEEECCCCCHHEECCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCC
PDIEGRCQEGFCVLGQEVLKSAVQNPAELTINITVNGSVKKSYVHLAMKHSIPELISFLS
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
QFIAFKAGDILLTGTEDMPLLVGAGDQVSVEFAQLGCLTNTVTE
HHHHHCCCCEEEECCCCCEEEEECCCCEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA