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The map label for this gene is hpaG_2 [H]

Identifier: 15603394

GI number: 15603394

Start: 1728039

End: 1728803

Strand: Direct

Name: hpaG_2 [H]

Synonym: PM1529

Alternate gene names: 15603394

Gene position: 1728039-1728803 (Clockwise)

Preceding gene: 15603393

Following gene: 15603395

Centisome position: 76.55

GC content: 43.27

Gene sequence:

>765_bases
ATGAGTAAATATGCAAAAGTGATGTATCAAAATCAAATCCATAGCGTAGAAGTGCGACCAAATGGCTTGTTAACCGATCA
ACAACAATTATTGCCGAGTGAAGCGGTAACCTGGTTACCGCCAGCCAGTGGCATGATGTATGCCTTAGGCTTGAATTACG
CCGATCACGCCTCTGAATTAGATTTTAAGCCACCTGAAAAGCCCCTTGTGTTTGTGAAAACCCATCACACTTACACCGGT
CACAACAGCACCACATGGCGCCCTGATAATGTGGACTATATGCATTATGAATGTGAGCTGGTGGTGGTGATTGGTAAAAC
CGCAAAAAATGTAAAACGGGATGAGGCGATGGATTATGTGGCGGGTTATACCCTGTGTAATGACTATGCGATTCGTGATT
ATTTGGAGAACTATTATCGCCCAAATTTACGCGTGAAAAATCGTGTTGGCACGACCCCTGTTGGTCCTTGGATTGTGGAT
AAAGCGGATGTGAAAGATCCGATGAACTTAACTTTAAGAACATGGGTCAATGGCGAGTTGTGTCAAGAAGGGAATACCAA
AGACATGATCTTTGATATTGCGTACCTCATTGAACACCTCTCCCATATTACTACATTACAACCGGGCGATATGATCGCCA
CAGGCACACCAAAAGGGTTAGCAGATGTGAAACCGGGTGATTGCGTAGAAATTGAAATTGAGCAAATTGGCAAATTGACG
AACTACATTGTGAGTGAAACAGCCTTTTTTGCGCAAAACGAATAA

Upstream 100 bases:

>100_bases
GATATGCCACTTCTTGTGGGGGCTGGCGATCAGGTATCCGTTGAGTTTGCTCAACTGGGTTGTTTAACCAATACGGTAAC
TGAATGAGGTAAGCAGGATT

Downstream 100 bases:

>100_bases
AGAGAAAAGGAGACAACCCTATGATTAAGCATTGGATAAATGGTAAAGAAGTTGCCAGTGAAGAAACCTTTGATAATTTT
AATCCTGCAACAGGTGAAGT

Product: HpaG

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSKYAKVMYQNQIHSVEVRPNGLLTDQQQLLPSEAVTWLPPASGMMYALGLNYADHASELDFKPPEKPLVFVKTHHTYTG
HNSTTWRPDNVDYMHYECELVVVIGKTAKNVKRDEAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKNRVGTTPVGPWIVD
KADVKDPMNLTLRTWVNGELCQEGNTKDMIFDIAYLIEHLSHITTLQPGDMIATGTPKGLADVKPGDCVEIEIEQIGKLT
NYIVSETAFFAQNE

Sequences:

>Translated_254_residues
MSKYAKVMYQNQIHSVEVRPNGLLTDQQQLLPSEAVTWLPPASGMMYALGLNYADHASELDFKPPEKPLVFVKTHHTYTG
HNSTTWRPDNVDYMHYECELVVVIGKTAKNVKRDEAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKNRVGTTPVGPWIVD
KADVKDPMNLTLRTWVNGELCQEGNTKDMIFDIAYLIEHLSHITTLQPGDMIATGTPKGLADVKPGDCVEIEIEQIGKLT
NYIVSETAFFAQNE
>Mature_253_residues
SKYAKVMYQNQIHSVEVRPNGLLTDQQQLLPSEAVTWLPPASGMMYALGLNYADHASELDFKPPEKPLVFVKTHHTYTGH
NSTTWRPDNVDYMHYECELVVVIGKTAKNVKRDEAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKNRVGTTPVGPWIVDK
ADVKDPMNLTLRTWVNGELCQEGNTKDMIFDIAYLIEHLSHITTLQPGDMIATGTPKGLADVKPGDCVEIEIEQIGKLTN
YIVSETAFFAQNE

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=231, Percent_Identity=36.3636363636364, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI40786394, Length=231, Percent_Identity=35.9307359307359, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI215422413, Length=199, Percent_Identity=39.1959798994975, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI13654274, Length=198, Percent_Identity=39.3939393939394, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=39.6907216494845, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1787428, Length=182, Percent_Identity=32.967032967033, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557057, Length=191, Percent_Identity=34.5549738219895, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324161, Length=240, Percent_Identity=27.5, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28572127, Length=229, Percent_Identity=39.3013100436681, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24663695, Length=239, Percent_Identity=34.7280334728033, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI28571789, Length=205, Percent_Identity=36.5853658536585, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28794; Mature: 28663

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYAKVMYQNQIHSVEVRPNGLLTDQQQLLPSEAVTWLPPASGMMYALGLNYADHASEL
CCHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCCEEECCCCCCEEEEEECCCCCCCCCC
DFKPPEKPLVFVKTHHTYTGHNSTTWRPDNVDYMHYECELVVVIGKTAKNVKRDEAMDYV
CCCCCCCCEEEEEECCEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHH
AGYTLCNDYAIRDYLENYYRPNLRVKNRVGTTPVGPWIVDKADVKDPMNLTLRTWVNGEL
HHHHHHCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCHH
CQEGNTKDMIFDIAYLIEHLSHITTLQPGDMIATGTPKGLADVKPGDCVEIEIEQIGKLT
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHH
NYIVSETAFFAQNE
HHHHHHHHEEECCC
>Mature Secondary Structure 
SKYAKVMYQNQIHSVEVRPNGLLTDQQQLLPSEAVTWLPPASGMMYALGLNYADHASEL
CHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCCEEECCCCCCEEEEEECCCCCCCCCC
DFKPPEKPLVFVKTHHTYTGHNSTTWRPDNVDYMHYECELVVVIGKTAKNVKRDEAMDYV
CCCCCCCCEEEEEECCEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHH
AGYTLCNDYAIRDYLENYYRPNLRVKNRVGTTPVGPWIVDKADVKDPMNLTLRTWVNGEL
HHHHHHCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCHH
CQEGNTKDMIFDIAYLIEHLSHITTLQPGDMIATGTPKGLADVKPGDCVEIEIEQIGKLT
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHH
NYIVSETAFFAQNE
HHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA